Product Highlights
- Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets
- Reproducible – consistent results between technical replicates for increased confidence in results
- Robust – reliable detection of RNA targets from a broad range of sample types
- Fast – delivers reproducible, accurate assay results in as little as 40 minutes
- Efficient – excellent performance in multiplex assays
- Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
Product Description
The SensiFAST Probe Hi-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. SensiFAST Probe One-Step has been optimized to deliver excellent results in both singleplex and multiplex assays.
An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.
The SensiFAST™ Probe Hi-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX. SensiFAST Probe One-Step has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes.
Applications
- Gene expression analysis
- Pathogen detection
- RNA viral pathogen detection
- Genetic profiling
- miRNA profiling / quantification
- Genetically modified organisms (GMO) characterization

Introduction to SensiFAST
Overview, features and benefits of the SensiFAST product familyReal-Time PCR Selection Chart
One-step Vs. Two-step real-time RT PCR
A discussion of the pros and cons of each detection strategy.ebiomall.com
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不知道有多少这样的学生呢,其实我们做学生时也没人特意教,能用旋蒸都是很了不起的,老师不教也会主动学习,偷学或查资料
做实验常规试剂的沸点背不得很糟糕,但背得后问我为什么在旋蒸上蒸乙醇用60度,不用78度,我就吐血了
只好普及知识,借用BUCHI的介绍,这么有名的公司没人知道也是很菜的
羊毛冷凝器
2500年以前,由于巧合,希腊水手在公海上发现了羊毛冷凝器。当时,船只遇难,船员必须从盐水中获得重要的淡水。为此,他们将海水加热,并利用固定在罐子上方的羊毛将蒸汽冷凝。这种名为“羊毛冷凝器”的东西从古代广泛传播到现代,与较早的“瓷碗变体”比较,代表了一种巨大的进步。
羊毛冷凝器.JPG(6.82k)
我用乙醇来提取,但是回收瓶中只有乙醇,那里出问题了呢????
请问有什么方法可以不漏气呢?
谢谢大家!!
1.我利用氯仿萃取生物碱,需要将氯仿蒸干,我做的样品有非常多份,但是每份样品很少,用的氯仿也很少,每份只用十几毫升左右,但是旋转蒸发仪的蒸发瓶好大,而且一次只能蒸发一个样,太慢了,就算蒸发完了,取出生物碱的时候也不好取,由于我做的是定量试验,所以如果取出生物碱时有残留会影响实验结果,到底怎么办啊?有没有特制的小的蒸发瓶?或者特制的可以一次接好几个蒸发容器的接口?
2.请问恒温水浴锅可不可以用来做蒸发?由于是蒸发氯仿,担心没有回收装置会导致泄露中毒的问题,但是我的量比较少,又想冒险试试,真是焦头烂额了现在,大家把那个我出出主意吧!!!!
已经试过的方法有:电吹风烘箱
将试的方法是:超声
大家有什么好建议,谢谢了!
祝大家元宵节快乐!

