BECarginase inhibitor |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
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- Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
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Chemical structure
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Cas No. | 63107-40-4 | SDF | Download SDF |
Chemical Name | S-(2-boronoethyl)-L-cysteine | ||
Canonical SMILES | OB(O)CCSC[C@@H](C(O)=O)N | ||
Formula | C5H12BNO4S | M.Wt | 193.0 |
Solubility | ≤5mg/ml in PBS(pH7.2) | Storage | Store at -20°C |
Physical Appearance | A crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. |
K(I): 0.4-0.6 microM
S-(2-boronoethyl)-L-cysteine (BEC) is an arginase inhibitor.
Arginases can catalyze the hydrolysis of L-arginine to yield L-ornithine and urea. Recently, studies show that arginases, both the type I and type II isozymes, involve in the regulation of nitric oxide production via modulating the availability of arginine for nitric oxide synthase.
In vitro: Although BEC has been first identified as inhibitor of type I arginase, it was found to be a classical, competitive inhibitor of human type II arginase with K(i) value 0.31 microM at pH 7.5. However, at pH 9.5, BEC was a slow-binding inhibitor of the enzyme with K(i) value 30 nM [1].
In vivo: In animal study, the administration of BEC was found to be able to decrease arginase activity and cause alterations in NO homeostasis, which were indicated by increases in S-nitrosylated and nitrated proteins in the lungs from inflamed mice. Moreover, in contrast to first expectations, BEC could enhance perivascular and peribronchiolar lung inflammation, NF-κB DNA binding, mucus metaplasia, and mRNA expression of the NF-κB-driven chemokine genes including KC and CCL20, and result in further increases in airways hyperresponsiveness [2].
Clinical trial: Up to now, BEC is still in the preclinical development stage.
References:[1] N. N. Kim, J. D. Cox, R. F. Baggio, et al. Probing erectile function: S-(2-boronoethyl)-L-cysteine binds to arginase as a transition state analogue and enhances smooth muscle relaxation in human penile corpus cavernosum. Biochemistry 40, 2678-2688 (2001).[2] Ckless K et al. Inhibition of arginase activity enhances inflammation in mice with allergic airway disease, in association with increases in protein S-nitrosylation and tyrosine nitration. J Immunol. 2008 Sep 15;181(6):4255-64.
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如果必要,安装的时候有什么要求?比如尺寸,安装位置等