주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
|---|
제품특징
□ 특징
● No-waiting workflow - 실온에서 5분이면 His-tag 단백질 정제 가능
● High purity and yield - 1개의 column당 100ug, 농도 ~ 0.3 - 1 mg/ml의 정제 가능
● Wide range of conditions - Native and denaturing conditions; with common additives
(e.g., EDTA, DTT, βME, glycerol, TCEP, etc.)
● Lysis buffer compatibility - xTractor Buffer 등 다양한 Lysis buffer 사용 가능
● Different cell systems - Mammalian cell, bacterial cell 등 다양한 샘플 적용 가능
□ 제품설명
Next-generation membrane technology makes the His-TaggedPurification Miniprep Kit a revolutionary solution for recombinant proteinpurification, capable of outperforming conventional methods under a wide rangeof conditions. Where resin columns fall short, this new system provides highcapacity and purity in just five minutes, from loading sample applicationthrough elution. Novel nylon membranes have been assembled into spin columns,creating a no-waiting,room-temperature workflow compatible with both mammalianand bacterial cells. Exceptional performance is consistent undera wide rangeof research settings, including denaturing conditions, in the presence ofadditives (e.g., βME, EDTA, DTT, glycerol, TCEP, etc.; see table below), andwhen performing tandem purification of his-tagged proteins.
그림1. Protein purification miniprep workflow. Eachmini spin column can be loaded with up to 800 μl of lysate (yielded from 2?5 mlof culture). His-tagged protein is first bound to the membrane, followed bywashing with 300 μl wash buffer, and elution with 300 μl elution buffer. Over90% of the bound protein can be eluted with as little as 100 μl elution buffer.Each step is followed by spinning the tube for 1 min at 11,000 x g. The workingbed volume of the membrane is <2 μl. This entire purification is complete in<5 min.
그림2. Purification of GFPuv under native and denaturing conditions. Spin columns were loadedwith 800 μl of cell lysate, and all stepswere performed with appropriate buffers for native and denaturing conditions. 8M urea or 6 M guanidine were included in appropriate samples.
□ Reagents Compatible with His-Tagged Purification Miniprep Kits
Reagent | Compatibility* |
MOPS | 200 mM |
HEPES | 200 mM |
Tris | 200 mM |
EDTA | 10 mM |
Beta-mercaptoethanol | 30 mM |
DTT | 10 mM |
TCEP | 5 mM |
Guanidine-HCl | 6 M |
Urea | 8 M |
Nonionic detergent (Triton X-100) | 2% |
Nonionic detergent (Tween 20) | 2% |
Anionic detergent (SDS) | 1% |
Arginine | 500 mM |
Glycine | 100 mM |
Histidine | 20 mM |
Sodium chloride | 2 M |
Imidazole | 40 mM |
Glycerol | 10% |
□ 적용
● Biochemical/enzymatic assays
● Pull-down or other proteininteraction assays
● Confirmation screening
● Protein/ligand affinityanalysis
● Crystallography or otherstructural analyses
● Antibody production
● Toxicology studies
● Protein transfer to live cells
□ 구성품 (자세한 내용은 CoA를 참조하세요)
● 20 ea Mini SpinColumns
● 2 x 15 ml xTractorBuffer
● 10 ml WashBuffer
● 10 ml ElutionBuffer
Capturem™ His-tagged protein purification kit 시리즈 비교
Miniprep Kit | Maxiprep Kit | 96well plate | Large volume | |
Code | 635710 | 635713 | 635714 | 635724 |
형태 |
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Input sample | ~ 800 ㎕ | ~ 25 ㎖ | ~ 1,000 ㎕ | 150 ~ 500 ㎖ |
용량 | 20 회 | 6 회 | 96 well | 4 회 |
소요시간 | 5 분 | 15 분 | 15 분 | 15 ~ 30 분 |
정제농도 | 0.3 ~ 1 ㎎/㎖ | 1.6 ~ 4.5 ㎎/㎖ | 0.3 ~ 1 ㎎/㎖ | up to 2.0 ㎎/㎖ |
정제수율 | 0.1 ㎎/column | 25 ㎎/tube | 0.1 ㎎/well | 25 ㎎/unit |
Centrifuge 조건 | 11,000 x g, 1분 | 2,000 x g, 3분* | 2,000 x g, 3분* | X |
Vacuum 여부 | X | X | O | O |
Buffer 포함 여부 | O | O | X | X |
Miniprep Column
Maxiprep column
96 Nickel plate 
Large volume unit* Maxiprep Kit에는 50㎖ tube용, 96well plate에는 96well plate용 centrifuge가 필요합니다.
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一般大气压以上5KPA的压力环境就能把水的沸点提高1度;
所以100KPA下的压力锅内的水沸点可以达到120度,所以在这里面杀菌就比在普通的锅里面杀菌效果要好,
当然针对那些怕高温的菌种了;
高压灭菌锅简介
高压灭菌锅又名高压蒸汽灭菌锅，可分为手提式灭菌锅和立式高压灭菌锅。利用电热丝加热水产生蒸汽，并能维持一定压力的装置。主要有一个可以密封的桶体，压力表，排气阀，安全阀，电热丝等组成。高压灭菌锅用途
适用于医疗卫生事业，科研，农业等单位，对医疗器械，敷料，玻璃器皿，溶液培养基等进行消毒灭菌，是理想的设备。手提式高压灭菌锅是食品厂、饮用水厂办QS、HACCP认证的必备检验设备.
高压灭菌锅控制方式
采用微电脑智能化全自动控制，控制灭菌压力，温度，时间；超温自动保护置：超过设定温度，自动切断加热电源；门安全连锁装置：内腔有压力，门盖无法打开，专利装置；低水位报警：缺水时能自动切断电源，声光报警，进口断水检测装置；漏电保护：配置漏电保护装置；温度动态数字显示，灭菌结束发出结束信号；升温、灭菌、排汽、干燥过程自动控制，无须人工监管；模式一：加热-灭菌-排汽；模式二：加热-灭菌-脉冲排汽；模式三：加热-灭菌-不排汽，模式四：自定义。
高压灭菌锅如何使用
1、在外层锅内加适量的水，将需要灭菌的物品放入内层锅，盖好锅盖并对称地扭紧螺旋。
2、加热使锅内产生蒸气，当压力表指针达到 33.78kPa时，打开排气阀，将冷空气排出，此时压力表指针下降，当指针下降至零时，即将排气阀关好。
3、继续加热，锅内蒸气增加，压力表指针又上升，当锅内压力增加到所需压力时，将火力减小，按所灭菌物品的特点，使蒸气压力维持所需压力一定时间，然后将灭菌器断电或断火，让其自然冷后再慢慢打开排气阀以排除余气，然后才能开盖取物。
需要达到103.4kPa（1.05kg/cm2）蒸汽压下，温度达121.3℃的条件。高压蒸汽灭菌法 可杀灭包括芽胞在内的所有微生物。方法是将需灭菌的物品放在高压锅内，加热时蒸汽不外溢，高压锅内温度随着蒸汽压的增加而升高。在103.4kPa（1.05kg/cm2）蒸汽压下，温度达到121.3℃。因为蒸汽不能溢出，而增加了灭菌器内的压力，从而使沸点增高，得到高于100℃的温度。导致菌体蛋白质凝固变性而达到灭菌的目的。
下排气式：敷料，121摄氏度，30min，102.9kPa
器械：121摄氏度，20min，102.9kPa
预真空式：器械、敷料，132～134摄氏度，4min，205.8kPa
