ELISA Kit for Eosinophil Chemotactic Factor (ECF)
CCL11; SCYA11; Eotaxin 1; Eosinophil Chemotactic Protein 1; Chemokine C-C-Motif Ligand 11; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11
- Product No.SEA025Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range7.8-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 3.2pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 328 For more details, please contact local distributors!US$ 468 For more details, please contact local distributors!US$ 2106 For more details, please contact local distributors!US$ 3978 For more details, please contact local distributors!US$ 32760 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
This assay has high sensitivity and excellent specificity for detection of Eosinophil Chemotactic Factor (ECF).No significant cross-reactivity or interference between Eosinophil Chemotactic Factor (ECF) and analogues was observed.
Recovery of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
Matrices listed below were spiked with certain level of recombinant Eosinophil Chemotactic Factor (ECF) and the recovery rates were calculated by comparing the measured value to the expected amount of Eosinophil Chemotactic Factor (ECF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-102 | 84 |
| EDTA plasma(n=5) | 85-93 | 88 |
| heparin plasma(n=5) | 79-94 | 83 |
Precision of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Eosinophil Chemotactic Factor (ECF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-91% | 95-105% | 99-105% | 93-102% |
| EDTA plasma(n=5) | 87-102% | 92-105% | 80-103% | 98-105% |
| heparin plasma(n=5) | 94-104% | 78-101% | 80-102% | 94-105% |
Stability of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Eosinophil Chemotactic Factor (ECF)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eosinophil Chemotactic Factor (ECF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eosinophil Chemotactic Factor (ECF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eosinophil Chemotactic Factor (ECF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eosinophil Chemotactic Factor (ECF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA025Mu02 | Recombinant Eosinophil Chemotactic Factor (ECF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA025Mu01 | Recombinant Eosinophil Chemotactic Factor (ECF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA025Mu02 | Polyclonal Antibody to Eosinophil Chemotactic Factor (ECF) | WB; IHC; ICC; IP. |
| PAA025Mu01 | Polyclonal Antibody to Eosinophil Chemotactic Factor (ECF) | WB; IHC; ICC; IP. |
| SEA025Mu | ELISA Kit for Eosinophil Chemotactic Factor (ECF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA025Mu | Mini Samples ELISA Kit for Eosinophil Chemotactic Factor (ECF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA025Mu | CLIA Kit for Eosinophil Chemotactic Factor (ECF) | Chemiluminescent immunoassay for Antigen Detection. |
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表皮分布于幼嫩的根、茎、叶、花、果实和种子的表面，通常是一层由初生分生组织的原表皮分化而来的生活细胞（夹竹桃等植物的叶上表皮，具有多层生活细胞所组成的复表皮），是保护植株免于风、雨、病原微生物和虫害等伤害的初生保护组织。表皮以表皮细胞为主，另有保卫细胞、副卫细胞及外生物等，有些植物的表皮中还有特化的异细胞。表皮的形态特征是物种鉴定的依据之一。
t=RC，其中t是时程，R是电阻，C是电容，我不知道这个电阻是不是电转杯的电阻还是电转仪本身有个电阻，如果有哪位大侠知道能不能告诉?
联系方式：wuyixuansunny@sohu.com
而方波是一种数字信号，其电信号在一应时间内保持持续稳定的点击，并在点击后瞬间消失，对细胞的损伤较小，转化效率较高。
主要是用于酵母，细菌等的转化．
