CLIA Kit for Interleukin 10 (IL10)
CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor
- Product No.SCA056Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range0.69-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.29pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 588 For more details, please contact local distributors!US$ 840 For more details, please contact local distributors!US$ 3780 For more details, please contact local distributors!US$ 7140 For more details, please contact local distributors!US$ 58800 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Interleukin 10 (IL10)
This assay has high sensitivity and excellent specificity for detection of Interleukin 10 (IL10).No significant cross-reactivity or interference between Interleukin 10 (IL10) and analogues was observed.
Recovery of the CLIA Kit for Interleukin 10 (IL10)
Matrices listed below were spiked with certain level of recombinant Interleukin 10 (IL10) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 10 (IL10) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-99 | 96 |
| EDTA plasma(n=5) | 79-104 | 82 |
| heparin plasma(n=5) | 82-98 | 90 |
Precision of the CLIA Kit for Interleukin 10 (IL10)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the CLIA Kit for Interleukin 10 (IL10)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 10 (IL10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 97-105% | 97-105% | 90-99% |
| EDTA plasma(n=5) | 82-93% | 87-94% | 89-97% | 86-97% |
| heparin plasma(n=5) | 95-103% | 86-95% | 89-97% | 98-105% |
Stability of the CLIA Kit for Interleukin 10 (IL10)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Interleukin 10 (IL10)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
Test principle of the CLIA Kit for Interleukin 10 (IL10)
The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 10 (IL10) level in the sample or standard.;
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ELISA / CLIA Experiment Service
INCREMENT SERVICES
Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIACLIA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental ServiceStreptavidin
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APA056Hu01 | Active Interleukin 10 (IL10) | Cell culture; Activity Assays. |
| RPA056Hu01 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA056Hu02 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA056Hu61 | Active Interleukin 10 (IL10) | Cell culture; Activity Assays. |
| EPA056Hu61 | Eukaryotic Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA056Hu02 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| PAA056Hu01 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| PAA056Hu06 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| LAA056Hu71 | Biotin-Linked Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC. |
| MAA056Hu22 | Monoclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| MAA056Hu21 | Monoclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| HEA056Hu | High Sensitive ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA056Hu | ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA056Hu | Mini Samples ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA056Hu | CLIA Kit for Interleukin 10 (IL10) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA056Hu01 | ELISA Kit DIY Materials for Interleukin 10 (IL10) | Main materials for "Do It (ELISA Kit) Yourself". |
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谢谢各位！
不过制冰机里的冰不可以，那冰怎么得到呢？
protocol上面说在离心菌液的时候离心管里面要有1/2的冰啊，这个冰应该是碎冰而不是直接冻出来的整块的冰吧。
大家是怎么得到的呢？谢谢！！
制冰机原理：
制冰机是一种将水通过蒸发器由制冷系统制冷剂冷却后生成冰的制冷机械设备。根据蒸发器和生成过程方式原理不同，生成的冰的形状也不同，人们一般根据冰形状将制冰机分为颗粒冰机、片冰机、板冰机、管冰机、壳冰机等等。
制冰机的分类：
按冰的形状可分：鳞形片冰机，雪花制冰机，条冰机，板冰机，块冰机（可分食用小块冰机和工业用大块冰机），冰粒机，颗粒机，管冰机，子弹头制冰机。
实验室制冰机种类选择：
我们平时在外面就餐时，经常看到商家在饮料中加入冰块，有时候是方块的，有时候是中空的，还有在菜市场买海鲜的时候也会看到下面铺着一层冰，究竟有什么区别吗？我们在开展实验室工作的时候，应如何选择制冰机呢？
实验室制冰机主要是用于在开展生命科学相关实验时，给样品提供冰浴环境，防止样品（如核酸、蛋白）因为温度的变化导致降解或变质，从而保障实验结果的准确性和有效性。绝大部分的时候，我们的样品是放在EP管、离心管、pcr管等容器里面，然后把样品管插在冰浴中，靠接触来实现温度的保持。由于样品和冰无法直接接触，而是要通过管壁来传输，所以管壁和冰浴的接触面积是冰浴效果的保障，为了更好的保障样品，应尽量的扩大管壁和冰浴的接触面积。
针对以上考虑，实验室制冰机一般选择雪花制冰机，该种类型的制冰机生产出来的冰呈雪花状碎冰，由于颗粒很小，可以和管壁进行密切的接触，保障冰浴效果。
对于出冰的具体要求中，还有一点是非常重要的，出来的雪花冰要求有15-25%的含水量，这样可极大的提高冰浴和管壁的接触效果。
文章来源：厦门国仪科学仪器有限公司
