![SMOBIO/[DL3000] ExcelDye™ 6X DNA Loading Dye, Blue, 5 ml x 2/Blue, 5 ml x 2/DL3000](images/SMOBIO/image.jpg)
Description
The ExcelDye™ 6× DNA Loading Dye (Blue) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
0.03% Xylene cyanol FF
0.03% Bromophenol blue
10 mM Tris-HCl (pH 8.0)
60% glycerol
60 mM EDTA
Storage
4°C for 12 months-20°C for 36 months
Contents
Component | Volume |
ExcelDye 6X DNA Loading Dye, Blue | 2 × 5ml |
Heading Sample
Manual
Manual_DL3000_ExcelDye™ 6X DNA Loading Dye, Blue
SDS
SDS_DL3000
ExcelBand™ DNA Ladder series
FluoroBand™ DNA Ladder series
AccuBand™ DNA Marker series
FluoroVue™ Nucleic Acid Gel Stain
Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light
FluoroStain™ DNA Fluorescent Staining Dye
Excellent for post staining
Sensitivity up to 0.04 ng DNA
A safe alternative to EtBr
Suitable for blue or UV light
FluoroDye™ DNA Fluorescent Loading Dye
Excellent for premix with DNA sample
Sensitivity up to 0.14 ng DNA
Safety dye
Convenience - monitor the electrophoresis in real-time
ebiomall.com
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概括了以下四点:
1.在用完制冰机后需要将冰箱内的冰块清理干净。清洗制冰机时应关掉电源，不要用水管直接对准机身冲洗，用清水，加入适量中性洗涤剂(严禁用酸性、碱性等腐蚀性溶剂)擦拭机器围板和内胆，再用软布擦干净。
2.制冰机必须两个月旋开进水软管管头，清洗进水阀滤网，避免水中砂泥杂质堵塞进水口，而引起进水量变小，导致不制冰。
3.每二个月清扫冷凝器表面灰尘，冷凝散热不良会引起压缩机部件损坏。清扫时，使用吸尘器、小毛刷等清洗冷凝表面油尘，不能使用尖锐金属工具清扫，以免损坏冷凝器。
4.制冰机的水管、水槽、储冰箱及保护胶片要每两个月清洗一次。不使用时，应清洗干净，并用电吹风吹干冰模及箱内水分，放在无腐蚀气体及通风干燥的地方，避免露天存放。
不过制冰机里的冰不可以，那冰怎么得到呢？
protocol上面说在离心菌液的时候离心管里面要有1/2的冰啊，这个冰应该是碎冰而不是直接冻出来的整块的冰吧。
大家是怎么得到的呢？谢谢！！
谢谢各位！
