BrainFectIN™ enables nucleic acids delivery into central nervous system of small animals.
Major difficulties with gene delivery in the central nervous system is the weakness of standard non-viral gene carriers and the limitations associated to the use of viral particles (time-consuming and requires additional saftey precautions). Unlike these methods, BrainFectIN™ is an original non-viral formulation that allows safe, easy and efficient nucleic acids delivery into central nervous system of small animals.
This transfection reagent allows transfection of neural cells in specific brain following stereotaxic injection, with low immunogenicity and rapid and long-term transgene expression.
BrainFectIN™ has been designed by our R&D team to meet in vivo grade quality (reagents performed under high manufacturing and quality standards and tested by strict quality controls).
BrainFectIN™ main advantages:
- Targeted process (stereotaxic injection)
- Good transfection efficiency
- Reduction of the injection volume
- Reduction of the DNA doses
- Minimized toxicity
- Low immunogenicity
- Rapid and long-term transgene expression
Sizes:
- 100 µL of BrainFectIN™ / 20-30 injections
- 250 µL of BrainFectIN™ / 40-60 injections
- 500 µL of BrainFectIN™ / 80-120 injections
Storage: -20°C.Shipping condition: room temperature.
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概括了以下四点:
1.在用完制冰机后需要将冰箱内的冰块清理干净。清洗制冰机时应关掉电源,不要用水管直接对准机身冲洗,用清水,加入适量中性洗涤剂(严禁用酸性、碱性等腐蚀性溶剂)擦拭机器围板和内胆,再用软布擦干净。
2.制冰机必须两个月旋开进水软管管头,清洗进水阀滤网,避免水中砂泥杂质堵塞进水口,而引起进水量变小,导致不制冰。
3.每二个月清扫冷凝器表面灰尘,冷凝散热不良会引起压缩机部件损坏。清扫时,使用吸尘器、小毛刷等清洗冷凝表面油尘,不能使用尖锐金属工具清扫,以免损坏冷凝器。
4.制冰机的水管、水槽、储冰箱及保护胶片要每两个月清洗一次。不使用时,应清洗干净,并用电吹风吹干冰模及箱内水分,放在无腐蚀气体及通风干燥的地方,避免露天存放。
不过制冰机里的冰不可以,那冰怎么得到呢?
protocol上面说在离心菌液的时候离心管里面要有1/2的冰啊,这个冰应该是碎冰而不是直接冻出来的整块的冰吧。
大家是怎么得到的呢?谢谢!!
谢谢各位!

