The NSET™ Device is manufactured in the USA by an FDA Registered Medical Device Manufacturer and ISO 13485:2003 Registered Company and is EtO (Ethylene Oxide) sterilization processed. Patent Information: Non-Surgical Embryo Transfer Method and Apparatus, United States Patent 9,615,903.[PDF]
A video demonstrating the use of the NSET device for mice can be found below.
Click on the Merit Ribbon to view our Award Winning Poster during D5 AALAS 2014, Stone B. (2014) “A Non-surgical Uterine Transfer Technique for Mouse Embryos after Cryopreservation, In Vitro Fertilization, ES-cell Injection, and Sperm during Artificial Insemination.”
Non-Surgical Embryo Transfer Device for MicePlace an order by completing the PDF order form and returning it to ParaTechs. All prices are USD. Shipping and Handling are added to invoice at time of processing. | |||
| Product number: 60010 | 10 Devices/box | 1-9 boxes = $250 ea.10-49 boxes = $225 ea.50+ boxes = $200 ea. |  |
The NSET Demonstration VideoThe demonstration video can be downloaded. This video demonstrates how easily mouse uterine embryo transfer can be accomplished using the NSET™ (Non-Surgical Embryo Transfer) Device for Mice.
ParaTechs’ Non-Surgical Embryo Transfer (NSET™) Device revolutionizes mouse uterine embryo transfer by reducing cost and eliminates the need for animal anesthesia and recovery normally required for conventional surgical procedures.
Uses•Embryo transfer after DNA microinjection•ES cell chimeric blastocyst transfer•Cryopreserved embryo transfer•Embryo transfer after in vitro fertilization•Embryo transfer for rederivation•Pathogen or material transfer to uterine horn•Sperm transfer for artificial insemination (AI) | Advantages•Eliminates the pain and distress of surgery•No anesthesia required•Eliminates need for post-surgical monitoring of animals•Reduces regulatory burden by eliminating need to justify survival surgery•Eliminates surgical instruments and time-consuming pre- and post-surgical processes•Greatly reduces time required to become proficient in embryo transfer or artificial insemination (AI)•Reduces costs of embryo transfer•Reduces costs of artificial insemination (AI) |
Compare this methodology with the time, expense, and training required to accomplish the same task with other methods and you will discover it is humane, quick and cost effective.
• NSET Technical Support Letter [PDF Only]
• NSET Instructions [PDF] REVISED JAN2016
• NSET Helpful Hints [PDF] REVISED JAN2016
• NSET Frequently Asked Questions [PDF] REVISED JAN2016
• NSET Publications
• NSET Presentations
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蛋白质上样缓冲液中的SDS和DTT分别可以屏蔽蛋白质电荷以及破坏二硫键，避免形成多聚体。
DNA上样缓冲液主要是起沉降和指示作用，不可用于蛋白，否则会影响蛋白相对分子量定量。
同样蛋白上样缓冲液中的tris-HCl浓度过高也会使溴酚蓝条带扭曲。
我使用的是1%琼脂糖凝胶，电泳缓冲液为0.5×TBE。
请问各位大虾，在不影响实验结果的前提下，这样的含EB的电泳缓冲液可以反复使用多少次？
谢谢^_^
由弱酸及其盐、弱碱及其盐组成的混合溶液，能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响，从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
② 应取pH8.0，这样可使核苷酸带较多负电荷，利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷，但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时，根据核苷酸负电荷的多少来决定洗脱速度，则洗脱顺序为CMP>AMP> GMP > UMP，但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附，嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为：CMP> AMP > UMP >GMP。
1、根据所需求的酸碱性选择合适的缓冲对，若是配制酸性缓冲液就选择弱酸与弱酸盐缓冲对；若是配制碱性缓冲液就选择弱碱与弱碱盐缓冲对。
2、根据所需要控制的PH范围以及弱酸和弱碱的解离常数（pKa/pKb）选择具体的共轭酸碱对，公式为PH＝pKa±1＝（14-pKb）±1。
3、根据公式计算缓冲溶液的组分比，酸性缓冲液PH＝pKa－lgc酸/c盐，碱性缓冲液PH＝14-pKb+lgc碱/c盐。
4、根据共轭酸碱对以及其组分比配制缓冲液，方法同普通溶液。
举例：试配制一种缓冲液，体积为1L，PH能维持在10.25左右。
a、依题意选择弱碱与弱碱盐的共轭对；
b、由PH＝（14-pKb）±1，算出pKb在3.75与4.75之间，查弱碱的解离常数表可知氨水（pKb=4.75）符合要求，故可选择NH3-NH4Cl体系；
c、由PH＝14-pKb+lgc碱/c盐，算出c(氨水)/c(氯化铵)＝10；
d、设氨水的浓度为10mol/L，则氯化铵的浓度为1mol/L，所以在浓度为10mol/L体积为1L的氨水中加入1mol的氯化铵即可。
