The NSET™ Device is manufactured in the USA by an FDA Registered Medical Device Manufacturer and ISO 13485:2003 Registered Company and is EtO (Ethylene Oxide) sterilization processed. Patent Information: Non-Surgical Embryo Transfer Method and Apparatus, United States Patent 9,615,903.[PDF]
A video demonstrating the use of the NSET device for mice can be found below.
Click on the Merit Ribbon to view our Award Winning Poster during D5 AALAS 2014, Stone B. (2014) “A Non-surgical Uterine Transfer Technique for Mouse Embryos after Cryopreservation, In Vitro Fertilization, ES-cell Injection, and Sperm during Artificial Insemination.”
Non-Surgical Embryo Transfer Device for MicePlace an order by completing the PDF order form and returning it to ParaTechs. All prices are USD. Shipping and Handling are added to invoice at time of processing. | |||
Product number: 60010 | 10 Devices/box | 1-9 boxes = $250 ea.10-49 boxes = $225 ea.50+ boxes = $200 ea. |
The NSET Demonstration VideoThe demonstration video can be downloaded. This video demonstrates how easily mouse uterine embryo transfer can be accomplished using the NSET™ (Non-Surgical Embryo Transfer) Device for Mice.
ParaTechs’ Non-Surgical Embryo Transfer (NSET™) Device revolutionizes mouse uterine embryo transfer by reducing cost and eliminates the need for animal anesthesia and recovery normally required for conventional surgical procedures.
Uses•Embryo transfer after DNA microinjection•ES cell chimeric blastocyst transfer•Cryopreserved embryo transfer•Embryo transfer after in vitro fertilization•Embryo transfer for rederivation•Pathogen or material transfer to uterine horn•Sperm transfer for artificial insemination (AI) | Advantages•Eliminates the pain and distress of surgery•No anesthesia required•Eliminates need for post-surgical monitoring of animals•Reduces regulatory burden by eliminating need to justify survival surgery•Eliminates surgical instruments and time-consuming pre- and post-surgical processes•Greatly reduces time required to become proficient in embryo transfer or artificial insemination (AI)•Reduces costs of embryo transfer•Reduces costs of artificial insemination (AI) |
Compare this methodology with the time, expense, and training required to accomplish the same task with other methods and you will discover it is humane, quick and cost effective.
• NSET Technical Support Letter [PDF Only]
• NSET Instructions [PDF] REVISED JAN2016
• NSET Helpful Hints [PDF] REVISED JAN2016
• NSET Frequently Asked Questions [PDF] REVISED JAN2016
• NSET Publications
• NSET Presentations
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电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。
但是最好还是不要用,影响实验就不好了。
500ml
2。阴极缓冲液 ( 10 × ) :将 60.55g Tris ,89.58g Tricine 及 5g SDS 溶于400ml 蒸馏水中,加水至终体积为500ml
使用时稀释至1×的缓冲液,电泳槽内槽加入阴极电泳缓冲液,外槽加入阳极电泳缓冲液。形成Trcine-SDS-PAGE电泳系统。
配制分三步: 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。
2.配好的EDTA不灭菌行不?放4度冰箱等过完年回来直接拿去配TAE
3.TAE配好后不用灭菌吧