
Test Label: Alkaline PhosphataseTest Format: Double Antibody Sandwich (DAS) ELISACapture Reagent: PolyclonalDetection Reagent: Polyclonal
Intended Use:
The Agdia Bt-Cry3A ELISA was developed for the detection of or the absence of Cry3A protein expressed from NewLeaf® transgenic potato events.Bt endotoxins are proteins produced in plants or in culture expressed by genes taken from the bacterium, Bacillus thuringiensis. Using this test system, you can detect Bt endotoxin extracted from transgenic potato sprouts, tubers and leaves expressing the truncated Cry3A gene.Agdia GMO Trait ELISA"s are commonly used by plant breeders, universities and private testing labs throughout the world.NewLeaf® is a registered trade mark of Monsanto Company.
Includes:
- Antibody-coated 96-well microtiter plates
- Alkaline phosphatase enzyme conjugate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- Nonfat dried milk
- PBST buffer, 20X concentrated liquid
- Tween-20
- User Guide
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2乘SDS-PAGE上样缓冲液(20ml体系)
1.0mol/L Tris-HCL (pH 6.8) 2ml
1.0mol/L DTT 4ml
SDS 0.8g
溴芬蓝 0.04g
甘油 4ml
dd水 定容至20ml
4℃冰箱保存,可以不分装,我们实验室一般用三个月以上
先配1.0mol/L的DTT,双蒸无菌水溶解4℃冰箱保存,配上样缓冲液的时候加入就可以了,都混匀保存也没问题的
500ml
2。阴极缓冲液 ( 10 × ) :将 60.55g Tris ,89.58g Tricine 及 5g SDS 溶于400ml 蒸馏水中,加水至终体积为500ml
使用时稀释至1×的缓冲液,电泳槽内槽加入阴极电泳缓冲液,外槽加入阳极电泳缓冲液。形成Trcine-SDS-PAGE电泳系统。
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、

