
Test Label: NATest Format: ImmunoStrip®Capture Reagent: MonoclonalDetection Reagent: Monoclonal
Intended Use:
This ImmunoStrip® test is intended for seed quality purposes to determine the presence or absence of the drought resistance traits (CspB) in transgenic corn.This test system can be used to test individual corn seed and corn leaf or detect 1.0% transgenic CspB seed in a minimum sample size of 300 seeds.The CspB ImmunoStrip® has shown no cross-reaction with other transgenic proteins in corn seed and leaf including CP4 EPSPS (Roundup Ready® corn), Bt-Cry1Ab, Bt-Cry1F, Bt-Cry2A, Bt-Cry34Ab1, Bt-Cry3Bb1, mBt-Cry3A, PAT/bar, VIP3A, GA21 and GT21.
Includes:
- ImmunoStrips®
- PBST sample buffer not included, see related products
- User Guide
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电泳缓冲液的另一个作用是使溶液具有一定的导电性,以利于DNA分子的迁移,例如,一般电泳缓冲液中应含有0.01-0.04mol/L的Na+离子,Na+离子的浓度太低时电泳速度变慢;太高时就会造成过大的电流使胶发热甚至熔化。
电泳缓冲液还有一个组分是EDTA,加入浓度为1-2mmol/L,目的是螯合Mg2+ 等离子,防止电泳时激活 DNA酶,此外还可防止Mg2+离子与核酸生成沉淀。
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
2乘SDS-PAGE上样缓冲液(20ml体系)
1.0mol/L Tris-HCL (pH 6.8) 2ml
1.0mol/L DTT 4ml
SDS 0.8g
溴芬蓝 0.04g
甘油 4ml
dd水 定容至20ml
4℃冰箱保存,可以不分装,我们实验室一般用三个月以上
先配1.0mol/L的DTT,双蒸无菌水溶解4℃冰箱保存,配上样缓冲液的时候加入就可以了,都混匀保存也没问题的

