DESCRIPTION:
Afullyvalidated,highlyspecificrabbitpolyclonalantibodytoαandβisoformsofCaMKIIPhosphoThr-286(orThr287inβ)
Ca2+/calmodulin-dependentkinaseII(CaMKII)isaubiquitous,multifunctionalserine/threoninekinaseinvolvedintranslatingCa2+-transientsignalsintocellularresponses.FourseparateCaMKIIgenesareexpressedinman(α,β,δ,γ)withisoformswithineachgene.CaMKIIδandγareabundantintheheart.CaMKIIisactivatedbyCa2+/CaMbinding,andbecomesactivatedforprolongedperiodsoftimefollowingautophosphorylationofThr-286orThr-287(equivalentresiduesindifferentisoforms).Thisantibodyrecognizestheactive,autophosphorylatedenzyme(allisoforms)andthuscanmeasuretheabundanceofactiveCaMKII.ProlongedactivationofCaMKIIisassociatedwithavarietyofvascularandcardiacdiseasesincludingstroke,atrialfibrillationcardiacarrhythmia&suddendeath,cardiacapoptosis,andheartfailure.
Testedapplications: westernblot(1:5000dilution) Speciesrecognition: rat,humanandmouse.Expectedtoreactwithvariousisoformsfromchicken,cow,pig,rabbit,orangutan,zebrafish,ferretandXenopuslaevis.
DATASHEET:
| Description | Lyophilisedrabbitpolyclonalanti-serumtoCaMKIIPhosphoThr-286/287. |
| Immunogen | Syntheticpeptide(M281HRQET(PO3H2)VDC289)correspondingtoaminoacidssurroundingthephosphorylatedthreonineresidueatposition286(or287)ofCaMKII,whichwasconjugatedtokeyholelimpethemocyanin(KLH)bycarbodiimidecrosslinkage. |
| Isotype | IgG |
| Purification | Rawserum |
| Speciesreactivity | Reactswithrat.Notyettestedinotherspecies.However,fromprimarysequenceanalysis,theantibodywillrecognisePhosphoThr-286(or287)inallisoforms(α,β,γandδ)fromhuman,ratandmouse.Theantibodyisalsoexpectedtoreactwithvariousisoformsfromchicken,cow,pig,rabbit,zebrafish,andXenopuslaevis |
| Testedapplications | WB |
| Recommendeddilution | WB1:5000 |
| Optimization | Optimaldilutionstobedeterminedbyenduser |
| Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin20µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
| Storage | Lyophilised:4°CReconstituted:-80°C |
| Datasheet | A010-50 |
| MSDS | A010-50 |
| Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |
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常用流动相加酸碱后PH的总结,希望大家能够提供一点自己测过的结果,谢谢先
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液,再调pH到7.4;(我们试着用这个做了下,发现挂不上柱)
2.配置磷酸钠盐缓冲液:按NaH2PO4:Na2HPO4以19:81的摩尔比配制成pH7.4的缓冲液?(附一张百度出来的配方
)
3.如果是磷酸钠盐缓冲液,可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗?
再者,2和3这两个方法配制的磷酸钠盐缓冲液有什么区别?最终效果是一样的吗?如果不一样,有什么理论的知识支撑呢?个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外,我还想问一下,pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗?是怎么影响的?谢谢大家了!
有了源数据之后把源数据按照大小排列,
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图
因为是考察不同PH对药物的影响,样品又不好改变其PH值,这种情况怎么办?希望有经验的高手指教。
我的流动相是甲醇-水(90:10)
谢谢赐教!
请进子版按格式发贴,自行修改,谢谢。
由弱酸及其盐、弱碱及其盐组成的混合溶液,能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响,从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?
是否可以理解为纯化水得PH范围为6.3-7.6?能否直接用pH计测量?谢谢!

