InputType: | UnisolatedSamples |
ResearchArea: | DNADamage&Repair,DNAMethylation,NextGenSequencing |
TargetApplication: | SampleIsolation |
VesselFormat: | Plate/Tubes |
100%Guarantee: | 6months |
The EpiQuik™CirculatingCell-FreeDNA(cfDNA)IsolationEasyKit utilizesmagneticbeadsbasedsize-fractionationtechnologytoisolatecirculatingcell-freeDNA(cfDNA)fromplasma/serumsamplesinasimpleandfastmanner.TheisolatedcfDNAcanbedirectlyusedforrealtime-PCRandDNAlibrarypreparationsuitablefornextgenerationsequencing. Thekithasthefollowingadvantages:
BackgroundInformation Principle&Procedure StartingMaterials
References: 1.JahrSetal:CancerRes.2001,61:1659-1665
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Fig.2. HighrecoveryofcfDNA:DifferentamountsofHeLamononucleosomeswerespikedinto0.5mlofplasmathenisolatedusingtheEpiQuik™CirculatingCell-FreeDNA(cfDNA)IsolationEasyKit.TheisolatedDNAwasfluorescentlyquantified. Fig3. HighrecoveryofcfDNAconfirmedbybioanalyzeranalysis: 500ngofHelapolynucleosome(around3000bps)and500ngofmononucleosome(around170bps)weresimultaneouslyspikedinto0.5mlplasma andthenisolatedandsize-selected. Fig4. BioanalyzertraceofDNAlibrarypreparedfrom20ngofpurifiedmononucleosomesisolatedfromplasmaspikedwithbothmono-andpoly-nucleosomes.Librarypeaksize:350bps.
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