| InputType: | DNA |
| ResearchArea: | DNAMethylation |
| TargetApplication: | Immunoprecipitation |
| VesselFormat: | 96-WellPlate |
| 100%Guarantee: | 6months |
TheEpiQuik™HydroxymethylatedDNAImmunoprecipitation(hMeDIP)Kit isacompletesetofoptimizedbuffersandreagentstocarryoutasuccessfulhMeDIPprocedurefortheselectiveenrichmentofDNAfragmentscontaining5-hydroxymethylcytosine(5-hmC)inahighthroughputformatusingDNAisolatedfromanyspecies.ThehydroxymethylatedDNAthatisenrichedwiththiskitcanbeusedinvariousdownstreamapplicationsincludingPCR(hMeDIP-PCR)andmicroarrays(hMeDIP-chip)forinvestigatinggene-specifichydroxymethylationincellsortissues. About5-Hydroxymethylcytosine ProductFeatures
PrincipleandProcedure Easy,Fast,andFlexible SelectiveandHighEnrichment CompatibleWithMeDIP Responsive,Reliable,andPractical |
|
| EpiQuik™hMeDIP | Supplier1"shMeDIP | Supplier2"shMeDIP | |
| WorkingPrinciple | Immunocapture | Immunocapture | Immunocapture |
| WorkingFormat | Microplate-based | Beads-based | Beads-based |
| MaxNumberofReactionsPerRun | 96 | 10 | 16 |
| HighThroughputCapable | Yes | No | No |
| Convenience(AssaySteps) | 12steps | 20steps | 28steps |
| TotalProcedureTime | <3hours | ~24hours | ~24hours |
| PhysicalHandlingTime | <15minutes | >1hour | >1hour |
| AmountofInputDNA | 0.1-1µgss/dsDNA | 0.1-1µgss/dsDNA | 1.2µgss/dsDNA |
| FoldEnrichmentWithSpiked5-hmCControl | >1000 | >1000 | >1000 |
| FoldEnrichmentWithGenomicDNASamplesFromCells/Tissues | >100 | N/A | N/A |
| YieldofEnrichedhmeDNAfromGenomicDNASamples | ~4ngper500ngofhumanbrainDNA | N/A | N/A |
| Specificity(Positive/NegativeRatio) | >99% | >99% | >99% |
| CostPerReaction | $10 | $35 | $47 |
WashBuffer
AntibodyBuffer
hMeDIPSolution
DNAReleaseBuffer
Non-ImmuneIgG(0.6mg/ml)
5-hmCAntibody(0.6mg/ml)
ControlDNA(500ng/ml)
ProteinaseK(10mg/ml)
ControlPrimer-Forward(20µM)
ControlPrimer-Reverse(20µM)
8-WellAssayStrips(WithFrame)
Adhesive8-WellStripFilm
UserGuide
ebiomall.com
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常用流动相加酸碱后PH的总结,希望大家能够提供一点自己测过的结果,谢谢先
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液,再调pH到7.4;(我们试着用这个做了下,发现挂不上柱)
2.配置磷酸钠盐缓冲液:按NaH2PO4:Na2HPO4以19:81的摩尔比配制成pH7.4的缓冲液?(附一张百度出来的配方
)
3.如果是磷酸钠盐缓冲液,可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗?
再者,2和3这两个方法配制的磷酸钠盐缓冲液有什么区别?最终效果是一样的吗?如果不一样,有什么理论的知识支撑呢?个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外,我还想问一下,pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗?是怎么影响的?谢谢大家了!
有了源数据之后把源数据按照大小排列,
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图
因为是考察不同PH对药物的影响,样品又不好改变其PH值,这种情况怎么办?希望有经验的高手指教。
我的流动相是甲醇-水(90:10)
谢谢赐教!
请进子版按格式发贴,自行修改,谢谢。
由弱酸及其盐、弱碱及其盐组成的混合溶液,能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响,从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?
是否可以理解为纯化水得PH范围为6.3-7.6?能否直接用pH计测量?谢谢!


Fig.1.SchematicprocedureoftheEpiQuik™HydroxymethylatedDNAImmunoprecipitation(hMeDIP)Kit.
