| Application: | ELISA,IF,WB |
| AreasofResearch: | Immunology&Inflammation |
| Clonality: | Polyclonal |
| Conjugate: | Unconjugated |
| Host: | Rabbit |
| Isotype: | IgG |
| Purification: | >95%,ProteinGPurified |
| Reactivity: | Human,Mouse |
| 100%Guarantee: | 6months |
Background
Atypicalchemokinereceptorthatcontrolschemokinelevelsandlocalizationviahigh-affinitychemokinebindingthatisuncoupledfromclassicligand-drivensignaltransductioncascades,resultinginsteadinchemokinesequestration,degradation,ortranscytosis.Alsoknownasinterceptor(internalizingreceptor)orchemokine-scavengingreceptororchemokinedecoyreceptor.Hasapromiscuouschemokine-bindingprofile,interactingwithinflammatorychemokinesofboththeCXCandtheCCsubfamiliesbutnotwithhomeostaticchemokines.ActsasareceptorforchemokinesincludingCCL2,CCL5,CCL7,CCL11,CCL13,CCL14,CCL17,CXCL5,CXCL6,IL8/CXCL8,CXCL11,GRO,RANTES,MCP-1,TARCandalsoforthemalariaparasitesP.vivaxandP.knowlesi.MayregulatechemokinebioavailABIlityand,consequently,leukocyterecruitmentthroughtwodistinctmechanisms:whenexpressedinendothelialcells,itsustainstheabluminaltoluminaltranscytosisoftissue-derivedchemokinesandtheirsubsequentpresentationtocirculatingleukocytes;whenexpressedinerythrocytes,servesasbloodreservoirofcognatechemokinesbutalsoasachemokinesink,bufferingpotentialsurgesinplasmachemokinelevels.
Description
ACKR1PolyclonalAntibody.Unconjugated.Raisedin:Rabbit.
Formulation
Liquid.0.03%Proclin300,50%Glycerol,0.01MPBS,PH7.4.
Specificity
Human,Mouse
Isotype
IgG
UniprotID
Q16570
Purification
>95%,ProteinGpurified
Immunogen
RecombinanthumanAtypicalchemokinereceptor1protein(1-63AA)
Storage
Shippedat4°C.Upondeliveryaliquotandstoreat-20°C(short-term)or-80°C(long-term).Avoidrepeatedfreeze.
AlternativeNames
Atypicalchemokinereceptor1,Duffyantigen/chemokinereceptor,GlycoproteinD,Plasmodiumvivaxreceptor,ACKR1,DARC,FY,GPD
Application
ELISA,WB,IF;Recommendeddilution:WB:1:1000-1:5000,IF:1:50-1:200
VCU0006
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常用流动相加酸碱后PH的总结,希望大家能够提供一点自己测过的结果,谢谢先
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液,再调pH到7.4;(我们试着用这个做了下,发现挂不上柱)
2.配置磷酸钠盐缓冲液:按NaH2PO4:Na2HPO4以19:81的摩尔比配制成pH7.4的缓冲液?(附一张百度出来的配方
)
3.如果是磷酸钠盐缓冲液,可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗?
再者,2和3这两个方法配制的磷酸钠盐缓冲液有什么区别?最终效果是一样的吗?如果不一样,有什么理论的知识支撑呢?个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外,我还想问一下,pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗?是怎么影响的?谢谢大家了!
有了源数据之后把源数据按照大小排列,
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图
因为是考察不同PH对药物的影响,样品又不好改变其PH值,这种情况怎么办?希望有经验的高手指教。
我的流动相是甲醇-水(90:10)
谢谢赐教!
请进子版按格式发贴,自行修改,谢谢。
由弱酸及其盐、弱碱及其盐组成的混合溶液,能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响,从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?
是否可以理解为纯化水得PH范围为6.3-7.6?能否直接用pH计测量?谢谢!

