GsAF-1(alsotermedβ-theraphotoxin-Gr1b,GsAF-I)wasoriginallyisolatedfromthevenomofGrammostolaroseaspider.GsAF-Ipeptidetoxinisreportedtoblockthefollowingvoltage-gatedsodiumchannelisoforms:Nav1.1,Nav1.2,Nav1.3,Nav1.4,Nav1.6andNav1.7withrespectiveIC50valuesof 0.4,0.6,1.3,0.3,1.2and0.04µM.Inaddition,thetoxinblocksthehERG1isoformwithanIC50valueof4.8µM.
Description:
AAsequence:Tyr-Cys2-Gln-Lys-Trp-Leu-Trp-Thr-Cys9-Asp-Ser-Glu-Arg-Lys-Cys15-Cys16-Glu-Asp-Met-Val-Cys21-Arg-Leu-Trp-Cys25-Lys-Lys-Arg-Leu-NH2
Disulfidebonds:Cys2-Cys16,Cys9-Cys21,andCys15-Cys25
Length(aa):29
Formula:C160H245N47O41S7
MolecularWeight: 3707.48Da
Appearance:Whitelyophilizedsolid
Solubility:aqueousbuffer
CASnumber:notavailable
Source:Synthetic
Purityrate:>98%
Reference:
TargetPromiscuityandHeterogeneousEffectsofTarantulaVenomPeptidesAffectingNa+andK+IonChannels
Venom-derivedpeptidemodulatorsofionchannelgatingareregardedasessentialtoolsforunderstandingthemolecularmotionsthatoccurduringtheopeningandclosingofionchannels.Inthisstudy,wepresentthecharacterizationoffivespidertoxinson12humanvoltage-gatedionchannels,followingobservationsaboutthetargetpromiscuityofsomespidertoxinsandtheongoingrevisionoftheir“canonical”gating-modifyingmodeofaction.Thepeptideswerepurified denovo fromthevenomof Grammostolarosea tarantulas,andtheirsequenceswereconfirmedbyEdmandegradationandmassspectrometryanalysis.Theireffectsonseventetrodotoxin-sensitiveNa+ channels,thethreehuman ether-à-go-go (hERG)-relatedK+ channels,andtwohuman Shaker-relatedK+ channelswereextensivelycharacterizedbyelectrophysiologicaltechniques.AllthepeptidesinhibitedionconductionthroughalltheNa+channelstested,althoughwithdistinctivepatterns.ThepeptidesalsoaffectedthethreepharmaceuticallyrelevanthERGisoformsdifferently.Athigherconcentrations,allpeptidesalsomodifiedthegatingoftheNa+ channelsbyshiftingtheactivationtomorepositivepotentials,whereasmorecomplexeffectswererecordedonhERGchannels.Noeffectswereevidentonthetwo Shaker-relatedK+ channelsatconcentrationswellabovetheIC50 valuefortheaffectedchannels.Giventhesequencediversityofthetestedpeptides,weproposethattarantulatoxinsshouldbeconsideredbothasmultimodeandtarget-promiscuousionchannelmodulators;bothfeaturesshouldnotbeignoredwhenextractingmechaNISTicinterpretationsaboutionchannelgating.Ourobservationscouldalsoaidinfuturestructure-functionstudiesandmighthelpthedevelopmentofnovelionchannel-specificdrugs.
ELISARedaelli,etal.(2010)TargetPromiscuityandHeterogeneousEffectsofTarantulaVenomPeptidesAffectingNa+andK+IonChannels.JBC.PMID:19955179
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常用流动相加酸碱后PH的总结，希望大家能够提供一点自己测过的结果，谢谢先
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液，再调pH到7.4；（我们试着用这个做了下，发现挂不上柱）
2.配置磷酸钠盐缓冲液：按NaH2PO4：Na2HPO4以19：81的摩尔比配制成pH7.4的缓冲液？（附一张百度出来的配方
）
3.如果是磷酸钠盐缓冲液，可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗？
再者，2和3这两个方法配制的磷酸钠盐缓冲液有什么区别？最终效果是一样的吗？如果不一样，有什么理论的知识支撑呢？个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外，我还想问一下，pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗？是怎么影响的？谢谢大家了！
有了源数据之后把源数据按照大小排列，
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图
因为是考察不同PH对药物的影响，样品又不好改变其PH值，这种情况怎么办？希望有经验的高手指教。
我的流动相是甲醇-水（90：10）
谢谢赐教！
请进子版按格式发贴，自行修改，谢谢。
由弱酸及其盐、弱碱及其盐组成的混合溶液，能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响，从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?
是否可以理解为纯化水得PH范围为6.3-7.6？能否直接用pH计测量？谢谢!
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