Hainantoxin-III(HNTX-III;hainantoxin-3) isapeptidethathasbeenisolatedfromthevenomoftheChinesebirdspider Seleconosmiahainana. Hainantoxin-III specifically blocksmammalianneuronaltetrodotoxin-sensitivevoltage-gatedsodiumchannels (VGSCs). HainantoxinIII wasfoundinactiveontetrodotoxin-resistantVGSCsandvoltage-gatedCa2+channels(bothhighandlowvoltage-activated).
Hainantoxin-III stronglydepressedtheamplitudeofratDRGtetrodotoxin-sensitiveNa+ currentswithanIC50 valueof1.1nM.Like Hainantoxin-IV, Hainantoxin-III causesahyperpolarizingshiftofabout10mVinthevoltagemidpointofsteady-stateNa+ channelinactivation.Similarto Huwentoxin-IV, Hainantoxin-III and Hainantoxin-IV donotaffecttheactivationandinactivationkineticsofNa+ currents.
Hainantoxin-IIIinhibitsNav1.7 currentamplitudewithoutsignificantlyalteringtheactivationandinactivationkinetics.Hainantoxin-III increasesthedeactivationoftheNav1.7currentafterextremedepolarizations. Hainantoxin-III seemstointeractwithsite4andtotrapthedomainIIvoltagesensorintheclosedstate.TheinhibitionofNav1.7byhainantoxin-IIIisreversIBLeuponwashing,butnoreversibilitywasobservedfor Hainantoxin-IV and Huwentoxin-IV. Hainantoxin-IIIwasshowntoblock Nav1.1,Nav1.2,Nav1.3andNav1.7 expressedinHEK293cellswithIC50 valuesof1.27µM,275nM,491nMand232nM,respectively.
Description:
AAsequence: Gly-Cys2-Lys-Gly-Phe-Gly-Asp-Ser-Cys9-Thr-Pro-Gly-Lys-Asn-Glu-Cys16-Cys17-Pro-Asn-Tyr-Ala-Cys22-Ser-Ser-Lys-His-Lys-Trp-Cys29-Lys-Val-Tyr-Leu-NH2
Disulfidebonds: Cys2-Cys17,Cys9-Cys22,andCys16-Cys29
Length(aa): 33
Formula: C154H228N44O45S6
MolecularWeight: 3608.20Da
Appearance:Whitelyophilizedsolid
Solubility: waterandsalinebuffer
CASnumber: Notavailable
Source: Synthetic
Purityrate: >97%
Reference:
Inhibitionofneuronaltetrodotoxin-sensitiveNa+channelsbytwospidertoxins:hainantoxin-IIIandhainantoxin-IV.
Hainantoxin-IIIandhainantoxin-IV,isolatedfromthevenomoftheChinesebirdspiderSeleconosmiahainana,areneurotoxicpeptidescomposedof33-35residueswiththreedisulfidebonds.Usingwhole-cellpatch-clamptechnique,weinvestigatedtheiractiononionicchannelsofadultratdorsalrootganglionneurons.ItwasfoundthatthetwotoxinsdidnotaffectCa2+channels(bothhighvoltageactivatedandlowvoltageactivatedtypes)nortetrodotoxin-resistantvoltage-gatedNa+channels(VGSCs).However,hainantoxin-IIIandhainantoxin-IVstronglydepressedtheamplitudeoftetrodotoxin-sensitiveNa+currentswithIC50valuesof1.1and44.6nM,respectively.Bothhainantoxin-III(1nM)andhainantoxin-IV(50nM)causedahyperpolarizingshiftofabout10mVinthevoltagemidpointofsteady-stateNa+channelinactivation,buttheyshoweddifferenceinthereprimekineticsofVGSCs:hainantoxin-IIIsignificantlydecreasedtherecoveryratefrominactivationataprepulsepotentialof-80mVwhilehainantoxin-IVdidnotdo.Itisinterestingtonotethatsimilartohuwentoxin-IV,thetwohainantoxinsdidnotaffecttheactivationandinactivationkineticsofNa+currentsandataconcentrationof1microMtheycompletelyinhibitedtheslowinginactivationcurrentsinducedbyBMK-I(toxinIfromthescorpionButhusmartensiKarsch),ascorpionalpha-liketoxin.Theresultsindicatethathainantoxin-IIIandhainantoxin-IVarenovelspidertoxinsandaffectthemammalneuralNa+channelsthroughamechanismquitedifferentfromotherspidertoxinstargetingtheneuralreceptorsite3,suchasdelta-aractoxinsandmu-agatoxins.
XiaoY, etal.(2003)Inhibitionofneuronaltetrodotoxin-sensitiveNa+channelsbytwospidertoxins:hainantoxin-IIIandhainantoxin-IV. EurJPharmacol. PMID: 14512091
StructureandFunctionofHainantoxin-III,aSelectiveAntagoNISTofNeuronalTetrodotoxin-sensitiveVoltage-gatedSodiumChannelsIsolatedfromtheChineseBirdSpiderOrnithoctonushainana
Inthepresentstudy,weinvestigatedthestructureandfunctionofhainantoxin-III(HNTX-III),a33-residuepolypeptidefromthevenomofthespiderOrnithoctonushainana.Itisaselectiveantagonistofneuronaltetrodotoxin-sensitivevoltage-gatedsodiumchannels.HNTX-IIIsuppressedNav1.7currentamplitudewithoutsignificantlyalteringtheactivation,inactivation,andreprimingkinetics.Shortextremedepolarizationspartiallyactivatedthetoxin-boundchannel,indicatingvoltage-dependentinhibitionofHNTX-III.HNTX-IIIincreasedthedeactivationoftheNav1.7currentafterextremedepolarizations.TheHNTX-III·Nav1.7complexwasgraduallydissociateduponprolongedstrongdepolarizationsinavoltage-dependentmanner,andtheunboundtoxinreboundtoNav1.7afteralongrepolarization.Moreover,analysisofchimericchannelsshowedthattheDIIS3-S4linkerwascriticalforHNTX-IIIbindingtoNav1.7.ThesedataareconsistentwithHNTX-IIIinteractingwithNav1.7site4andtrappingthedomainIIvoltagesensorintheclosedstate.ThesolutionstructureofHNTX-IIIwasdeterminedbytwo-dimensionalNMRandshowntopossessaninhibitorcystineknotmotif.Structuralanalysisindicatedthatcertainbasic,hydrophobic,andaromaticresiduesmainlylocalizedintheCterminusmayconstituteanamphiphilicsurfacepotentiallyinvolvedinHNTX-IIIbindingtoNav1.7.Takentogether,ourresultsshowthatHNTX-IIIisdistinctfromβ-scorpiontoxinsandotherβ-spidertoxinsinitsmechanismofactionandbindingspecificityandaffinity.Thepresentfindingscontributetoourunderstandingofthemechanismoftoxin-sodiumchannelinteractionandprovideausefultoolfortheinvestigationofthestructureandfunctionofsodiumchannelisoformsandforthedevelopmentofanalgesics.
LiuZ., etal. (2013)StructureandFunctionofHainantoxin-III,aSelectiveAntagonistofNeuronalTetrodotoxin-sensitiveVoltage-gatedSodiumChannelsIsolatedfromtheChineseBirdSpiderOrnithoctonushainana. JBC. PMID: 23703613
Determinationofdisulfidebridgesoftwospidertoxins:Hainantoxin-IIIandHainantoxin-IV
WangW,etal.(2009) Determinationofdisulfidebridgesoftwospidertoxins:Hainantoxin-IIIandHainantoxin-IV. J.Venom.Anim. Toxinsincl.Trop.Dis
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
常用流动相加酸碱后PH的总结，希望大家能够提供一点自己测过的结果，谢谢先
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液，再调pH到7.4；（我们试着用这个做了下，发现挂不上柱）
2.配置磷酸钠盐缓冲液：按NaH2PO4：Na2HPO4以19：81的摩尔比配制成pH7.4的缓冲液？（附一张百度出来的配方
）
3.如果是磷酸钠盐缓冲液，可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗？
再者，2和3这两个方法配制的磷酸钠盐缓冲液有什么区别？最终效果是一样的吗？如果不一样，有什么理论的知识支撑呢？个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外，我还想问一下，pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗？是怎么影响的？谢谢大家了！
有了源数据之后把源数据按照大小排列，
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图
因为是考察不同PH对药物的影响，样品又不好改变其PH值，这种情况怎么办？希望有经验的高手指教。
我的流动相是甲醇-水（90：10）
谢谢赐教！
请进子版按格式发贴，自行修改，谢谢。
由弱酸及其盐、弱碱及其盐组成的混合溶液，能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响，从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?
是否可以理解为纯化水得PH范围为6.3-7.6？能否直接用pH计测量？谢谢!
暂无品牌分类
© 2005- 苏州蚂蚁淘生物科技有限公司 版权所有。
苏ICP备17049038号-11
苏公网安备 32059002001627号
声明：本网站部分产品受外界条件影响（包含但不限于进口国出口国政策限制等因素），无法提供销售服务，下单后，会由客服人员联系厂家确认后另行通知。部分危险品无法清关，请勿下单