Overview:
| ProductName | SODActivityKit |
| Description | Quantitativecolorimetricmeasurementofsuperoxidedismutaseactivity |
| SpeciesReactivity | SpeciesIndependent |
| Platform | Microplate |
| SampleTypes | Celllysates,EDTAPlasma,Erythrocytes,HeparinPlasma,Serum,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | DirectEnzymeActivityAssay |
| Utility | ColorimetricassayusedtoquantitativelymeasureSODactivityinavarietyofsamples. |
| Sensitivity | 0.044U/ml |
| AssayRange | 0.0625-4U/ml |
| Precision | IntraAssayPrecision:ThreesamplesdilutedinAssayBufferwereruninreplicatesof20inanassay.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-0.407U/mL,4.6%CVSample2-0.726U/mL,7.3%CVSample3-1.203U/mL,16.8%CVInterAssayPrecision:ThreesamplesdilutedinAssayBufferwereruninduplicatesinsixteenassaysrunovermultipledaysbyfouroperators.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-0.356U/mL,10.5%CVSample2-0.653U/mL,6.1%CVSample3-1.277U/mL,13.8%CV |
| NumberofSamples | 89samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||
| ProductType | ActivityKits | ||||||||||||||||||||||||
| AssayOverview | TheSuperoxideDismutase(SOD)ActivityKitisdesignedtoquantitativelymeasureSODactivityinavarietyofsamples.TheassaymeasuresalltypesofSODactivity,includingCu/Zn,Mn,andFeSODtypes.AbovineerythrocyteSODstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffofthestandardcurve.SamplesaredilutedinourspeciallycoloredSampleDiluentandaddedtothewells.TheSubstrateisaddedfollowedbyXanthineOxidaseReagentandincubatedatroomtemperaturefor20minutes.TheXanthineOxidasegeneratessuperoxideinthepresenceofoxygen,whichconvertsacolorlesssubstrateintheDetectionReagentintoayellowcoloredproduct.Thecoloredproductisreadat450nm.IncreasinglevelsofSODinthesamplescausesadecreaseinsuperoxideconcentrationandareductioninyellowproduct.TheactivityoftheSODinthesampleiscalculatedaftermakingasuitablecorrectionforanydilution,usingsoftwareavailablewithmostplatereaders.TheresultsareexpressedintermsofunitsofSODactivitypermL. | ||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | SODActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-214) |
BIOLOGicalDescription
| AlternativeNames | Cu/ZnSODActivityKit,SOD1ActivityKit,MnSODActivityKit,SOD2ActivityKit,EC-SODActivityKit,SOD3ActivityKit |
| ResearchAreas | Cancer,Alzheimer"sDisease,Atherosclerosis,CardiovascularSystem,Neurodegeneration,Neuroscience,OxidativeStress,Parkinson"sDisease |
| ScientificBackground | Short-livedandhighlyreactiveoxygenspecies(ROS)suchasO2·-(superoxide),·OH(hydroxylrADIcal),andH2O2(hydrogenperoxide)arecontinuouslygeneratedinvivo.Intherestingstate,thebalancebetweenantioxidantsandoxidantsissufficienttopreventthedisruptionofnormalphysiologicfunctions;however,eitherincreasesinoxidantsordecreasesinantioxidantscandisruptthisbalancegivingrisetoelevatedlevelsofreactiveoxygenspecies(ROS)(1,2).ThecellularlevelsofROSarecontrolledbyantioxidantenzymesandsmallmoleculeantioxidants.Themajorantioxidantenzymes,superoxidedismutases(SODs),includingcopper-zincsuperoxidedismutase(Cu/ZnSOD,SOD1),manganesesuperoxidedismutase(MnSOD,SOD2)andextracellularsuperoxidedismutase(EC-SOD,SOD3),allplaycriticalrolesinscavengingO2·-.DecreasedSODactivityresultsinelevatedlevelofsuperoxidewhichinturnleadstodecreasedNObutincreasedperoxynitriteconcentrations.ThemajorintracellularSODisa32-kDcopperandzinccontaininghomodimer(Cu/ZnSOD).ThemitochondrialSOD(MnSOD)isamanganese-containing93-kDhomotetramerthatissynthesizedinthecytoplasmandtranslocatedtotheinnermatrixofmitochondria.EC-SODistheprimaryextracellularSODenzymeandishighlyexpressedinmanyorgans.IncreasedSODactivitylevelsareseeninDownsSyndrome(3)whiledecreasedactivityisseenindiabetes,Alzheimer’sdisease,rheumatoidarthritis,Parkinson’sdisease,uremicanemia,atherosclerosis,somecancers,andthyroiddysfunction(3-8). |
| References | 1.Liocher,SIandFridovich,I.FreeRad.Biol.Med.,2007,42:1465-1469. 2.Imlay,JA.AnnRev.Bichem.,2008,77:755-776. 3.Torsdottir,G.etal.J.Neurol.Sci.2010.299(1-2):51-54. 4.Giacco,F&Brownlee,M.Circ.Res.2010.107(9):1058-1070. 5.Bae,S-C,etal.J.Amer.Coll.Nutr.2003.22(4):311-315. 6.Akbostanci,MC,etal.ActaNeurol.Belg.2001.101:180-183. 7.Shainkin-Kestenbaum,R,etal.Nephron.1990.55(3):251-253. 8.Saito,T.HokkaidoIgakuZasshi.1987.62(2):257-268. |
ProductImages

TypicalStandardCurveforSODActivityKit(EnzymeActivityAssay)StressXpress®–SKT-214.AssayType:DirectEnzyme.DetectionMethod:ColorimetricAssay.AssayRange:0.0625–4U/ml.

Linearitywasdeterminedbytakingtwosamples,onewithahighknownSODactivityandtheotherwithalowerSODactivityandmixingthemingivenratios.Themeasuredactivitieswerecomparedtotheexpectedvaluesbasedontheratiosused.

Short-livedandhighlyreactiveoxygenspecies(ROS)suchasO2·-(superoxide),·OH(hydroxylradical),andH2O2(hydrogenperoxide)arecontinuouslygeneratedinvivo.ThecellularlevelsofROSarecontrolledbyantioxidantenzymesandsmallmoleculeantioxidants.Themajorantioxidantenzymes,superoxidedismutases(SODs),includingcopper-zincsuperoxidedismutase(Cu/ZnSOD,SOD1),manganesesuperoxidedismutase(MnSOD,SOD2)andextracellularsuperoxidedismutase(EC-SOD,SOD3),allplaycriticalrolesinscavengingO2·-.
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因为是考察不同PH对药物的影响,样品又不好改变其PH值,这种情况怎么办?希望有经验的高手指教。
我的流动相是甲醇-水(90:10)
谢谢赐教!
请进子版按格式发贴,自行修改,谢谢。
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?
是否可以理解为纯化水得PH范围为6.3-7.6?能否直接用pH计测量?谢谢!
常用流动相加酸碱后PH的总结,希望大家能够提供一点自己测过的结果,谢谢先
有了源数据之后把源数据按照大小排列,
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液,再调pH到7.4;(我们试着用这个做了下,发现挂不上柱)
2.配置磷酸钠盐缓冲液:按NaH2PO4:Na2HPO4以19:81的摩尔比配制成pH7.4的缓冲液?(附一张百度出来的配方
)
3.如果是磷酸钠盐缓冲液,可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗?
再者,2和3这两个方法配制的磷酸钠盐缓冲液有什么区别?最终效果是一样的吗?如果不一样,有什么理论的知识支撑呢?个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外,我还想问一下,pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗?是怎么影响的?谢谢大家了!
1、弱酸和它的盐(如:HAc---NaAc)的水溶液组成;
2、弱碱和它的盐(如:NH3·H2O---NH4Cl)的水溶液组成;
3、多元弱酸的酸式盐及其对应的次级盐(如:NaH2PO4---Na2HPO4)的水溶液组成。
酸碱缓冲溶液的选型一般应根据具体情况进行选择。缓冲酸性可选用碱性缓冲液,缓冲酸性可采用碱性缓冲液。常用作缓冲溶液的酸类由弱酸及其共轭酸盐组合成的溶液具有缓冲作用。生化实验室常用的缓冲系主要有磷酸、柠檬酸、碳酸、醋酸、巴比妥酸、Tris(三羟甲基氨基甲烷)等系统,生化实验或研究工作中要慎重地选择缓冲体系,因为有时影响实验结果的因素并不是缓冲液的pH值,而是缓冲液中的某种离子。如硼酸盐、柠檬酸盐、磷酸盐和三羟甲基甲烷等缓冲剂都可能产生不需要的化学反应。
【酸碱缓冲溶液】由弱酸及其盐、弱碱及其盐组成的混合溶液,能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响,从而保持溶液的pH值相对稳定。这种溶液称为酸碱缓冲溶液。

