
Type:BlockingBuffer
Applications:E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysFormat:liquid
NeptuneBlockcontainsaproprietarynon-mammalianformulationbasedonfishproteins,plusmolecularstABIlizersthatprovideahighdegreeofblockingefficiency.NeptuneBlockwasdesignedforantigen-downELISAformats,andforsandwichassayswithhighbackgroundproblems.
Thisformulationisaheterogeneousmixtureofsmallmoleculescapableofblockingboththenonspecificbindingsitesoftheadsorbedprotein,aswellastheunoccupiedregionsofthepolystyreneplatethatarenotstericallyaccessIBLetolargertrADItionalmammalianblockingreagents,therebyreducingbackgroundnoise.Thesmallsizeoftheseuniqueblockersresultsinminimalsterichindrancetokeyepitoperegionsoftargetcoatedproteins,thusenhancingspecificsignalgeneration.
AsNeptuneBlockcontainsanon-mammalianproteinblockingagent,itisantigenicityforeigntomostmammalianimmunesystems.Inantigen-downELISAformats,thisreducesthepossibilityoffalse-positivesgeneratedfromendogenousantibodiesinthesamplereactingwithplateblockingproteins.NeptuneBlockisparticularlyusefulwhenworkingwithhuman,swine,andbovineserumsamples,asthereislittleinteractionwiththeblockingmoleculesresultinginlowerbackgrounds.
RecommendedUses:
- Antigen-downELISAs.
- SandwichELISAswithhighbackgrounds.
- ELISAstestinghumanandothermammaliansamples
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加样缓冲液的主要作用是使PCR产物与其混合,
使DNA沉于加样孔的底部,防止DNA跑出来.
0.5 M tris-HCl (pH 6.8) 20%
丙三醇 20%
20%SDS 20%
0.1%溴酚兰 5%
2-巯基乙醇 10%
双蒸水 25%
不调PH值,PH在配0.5 M tris-HCl 时已调,缓冲液呈蓝紫色。
蛋白电泳上样缓冲液包括2%SDS , 0.1%溴酚蓝 10%甘油,SDS是保证样品中的所有蛋白带电荷一致,减少电荷对电泳结果的影响。还原剂是让二硫键处于断开状态,保证蛋白分子的线性.
溴酚蓝作用是指示上样蛋白的跑胶时标记的,甘油是增加上样重量的,以免漂浮,煮沸是使蛋白变性的永久保存

