
Description
Details
NHS Ester Modified fluorescent dye with Absorption 653nm / Emission 672nm, suitable for protein labeling, microarray experiments, FisH microscopy, and gel electrophoresis. Spectral similar to Cy5™ and Alexa Fluor® 647 commonly used with RadiantDy™ 547. The increased water solubility of RadiantDy™ fluorescent dyes allows a high label to protein ratio without precipitation during the conjugation reaction.
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加样缓冲液的主要作用是使PCR产物与其混合,
使DNA沉于加样孔的底部,防止DNA跑出来.
蛋白质上样缓冲液中的SDS和DTT分别可以屏蔽蛋白质电荷以及破坏二硫键,避免形成多聚体。
DNA上样缓冲液主要是起沉降和指示作用,不可用于蛋白,否则会影响蛋白相对分子量定量。
同样蛋白上样缓冲液中的tris-HCl浓度过高也会使溴酚蓝条带扭曲。

