
Description
Details
NHS Ester Modified fluorescent dye with Absorption 653nm / Emission 672nm, suitable for protein labeling, microarray experiments, FisH microscopy, and gel electrophoresis. Spectral similar to Cy5™ and Alexa Fluor® 647 commonly used with RadiantDy™ 547. The increased water solubility of RadiantDy™ fluorescent dyes allows a high label to protein ratio without precipitation during the conjugation reaction.
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加样缓冲液的主要作用是使PCR产物与其混合,
使DNA沉于加样孔的底部,防止DNA跑出来.
0.5 M tris-HCl (pH 6.8) 20%
丙三醇 20%
20%SDS 20%
0.1%溴酚兰 5%
2-巯基乙醇 10%
双蒸水 25%
不调PH值,PH在配0.5 M tris-HCl 时已调,缓冲液呈蓝紫色。
蛋白电泳上样缓冲液包括2%SDS , 0.1%溴酚蓝 10%甘油,SDS是保证样品中的所有蛋白带电荷一致,减少电荷对电泳结果的影响。还原剂是让二硫键处于断开状态,保证蛋白分子的线性.
溴酚蓝作用是指示上样蛋白的跑胶时标记的,甘油是增加上样重量的,以免漂浮,煮沸是使蛋白变性的永久保存

