3D-FectIN™ is the newest 3D-Transfection Reagent specifically developed to directly transfect cells cultured in 3D hydrogels. 3D-FectIN™ is suitable for all kind of hydrogels and cells. 3D matrices not only add a third dimension to cells’ environment, they also allow creating significant differences in cellular characteristics and behaviours.
Because 3D matrices are routinely used in basic research and therapeutic applications, OZ Biosciences has developed 3D-FectIN™ transfection reagent specific for hydrogel. In this way, hydrogel-based 3D matrices activated with 3D-FectIN™/DNA complexes are colonized by cells which are subsequently transfected in situ and in a more natural environment. This method allows studying angiogenesis, tube and acini formation, colonization, neurite growth, tissue engineering, tissue regeneration, tumour invasion, neural differentiation, cellular polarization, tissue formation...
For Gene Silencing applications, please refer to si3D-FectIN
- Highly efficient: cell lines and primary cells
- Specific for 3D Hydrogels
- Compatible with all types of nucleic acids
- Completely biodegradable
- Long term protein expression
- Serum Compatible
Sizes:
- 250µL: Good for 65 transfections with 1µg of DNA
- 500µL: Good for 125 transfections with 1µg of DNA
- 1000µL: Good for 250 transfections with 1µg of DNA
Storage: +4°C
Shipping Conditions: Room temperature
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1。称量氨基丁三醇 242g,Na2EDTA.2H2O 37.2g 于1L烧杯中;
2。向烧杯中加入约600ml去离子水,充分搅拌均匀;
3。加入57.1ml的冰乙酸,充分溶解;
4。用NaOH调pH至8.3,加去离子水定容至1L后,室温保存。
使用时稀释50倍 即1×TAE Buffer
10×TBE Buffer配制方法:
1。称量氨基丁三醇 108g,Na2EDTA.2H2O 7.44g,硼酸55g 于1L烧杯中;
2。加入约700ml去离子水,搅拌均匀;
3。用NaOH调pH至8.3,加去离子水定容至1L后,室温保存。
使用时稀释10倍 即1×TBE Buffer
蛋白质上样缓冲液中的SDS和DTT分别可以屏蔽蛋白质电荷以及破坏二硫键,避免形成多聚体。
DNA上样缓冲液主要是起沉降和指示作用,不可用于蛋白,否则会影响蛋白相对分子量定量。
同样蛋白上样缓冲液中的tris-HCl浓度过高也会使溴酚蓝条带扭曲。
最好是测浓度,一般不超过30-40ug,上样缓冲液一般4×或者5×,这个没太大区别。
① 上样缓冲液中包括甘油,溴酚蓝,SDS等,甘油主要作用是防止样品漂浮出点样孔,溴酚蓝作为电泳指示剂,用来观察电泳进度,SDS用于一些核酸结合蛋白的变性解离.
②核酸染料与凝胶中核酸结合,以便于在紫外光下显示核酸条带.
0.5 M tris-HCl (pH 6.8) 20%
丙三醇 20%
20%SDS 20%
0.1%溴酚兰 5%
2-巯基乙醇 10%
双蒸水 25%
不调PH值,PH在配0.5 M tris-HCl 时已调,缓冲液呈蓝紫色。

