Peak Wavelengths | 650 nm, unstopped, blue reaction product450 nm, stopped, yellow reaction product |
---|---|
Reaction Temperature | Room temperature |
Substrate Concentration | 2.08 mmol/L |
This high sensitivity liquid substrate for peroxidase consists of Tetramethylbenzidine (TMB) plus dilute hydrogen peroxide in a single-reagent stabilized form. The reagent has been specifically formulated for measuring peroxidase in ELISA systems which is stable for long-term storage providing sensitivity greater than OPD.
This reagent is available as a single component, ready-to-use reagent. When used in conjunction with our proprietary TMB Stop Buffer (TSB), the resulting color product will not fade or darken for several hours.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转
我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
2.配好的EDTA不灭菌行不?放4度冰箱等过完年回来直接拿去配TAE
3.TAE配好后不用灭菌吧
如果你是制胶或者做电泳缓冲液的话,不用灭菌。