- Description
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Description
Details
Description:Mouse monoclonal antibody against Thr181 phosphorylated Tau protein
Purification:Protein G affinity purified
Target Protein:Human Thr181 phosphorylated Tau
Immunogen:KLH conjugated with a short peptide [Ala-Pro-Lys-Thr(p)-Pro-Pro-Ser-Ser-Gly-Glu-Cys], corresponding to the amino acid sequence 177-187 on the phosphorylated Tau protein.
Fusion Myeloma:Sp2/0-Ag14
Specificity:This antibody reacts with the unconjugated Thr181 phosphorylated Tau peptide.
Species Reacitvity:Human, mouse
Host / Isotype: Mouse, IgG2a Kappa
Formulation:Lyophilized from a solution in 0.01M PBS pH7.2
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL. Avoid repeated freeze and thaw cycle.
Storage: Store at -20oC
Research Area:Alzheimer’s disease and other neurodegenerative diseases
Background:
Tau is a highly soluble microtubule associated protein found in high concentration in neuron of central nervous system. The main function of the protein is stabilizing the microtubule in nerve fibre (axon). There are six tau isoforms which are formed by alternate splicing. Hyperphosphorylation of Tau protein results in aggregation and formation of neurofibrillary tangles, which is significantly associated with cognitive impairment in Alzheimer’s disease. Tau Thr181 phosphorylation has been used as a biomarker for the differentiation of Alzheimer’s disease and other primary dementia such as dementia with Lewy bodies..
Application:
1.Indirect ELISA: The antibody reacts with phosphorylated peptide [Ala-Pro-Lys-Thr(p)-Pro-Pro-Ser-Ser-Gly-Glu-Cys] coated ELISA plate in indirect ELISA. Cross-reactivity with un-phosphorylated peptide is very low.
2. Western Blot: The antibody detected a 46kD band in mouse brain tissue lysate.
Detect the mouse brain tissue lysates using antibody (Tau-pho181 7G9) at 1:2000 (0.5 μg/mL) dilution.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
Product Specificity | mAb anti-Thr181 Phosphorylated Tau, 7G9 |
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Application | EIA, WB |
Size | 0.1 mg |
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加样缓冲液的主要作用是使PCR产物与其混合,使DNA沉于加样孔的底部,防止DNA跑出来.
Tris-Glycine/SDS;
MOPS/SDS;
Bis-Glycine/SDS等不同的缓冲液、预混液等。
不知道这几种缓冲液有什么异同呢?
比如如果是Invitrogen的预制胶-预混液电泳系统,每种预制胶有适配缓冲液,但是如果用其他的缓冲液似乎也可以得到不错的结果,那么是否说明电泳缓冲液只要满足了缓冲ph范围,其他的不是特别重要呢?
此外,附加提问是,PVDF膜在转膜的时候,转膜缓冲液中不需要加入甲醇,那么是加入甲醇转膜效果好还是不加好呢?
谢谢!
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转