- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody against Thr181 phosphorylated Tau protein
Purification:Protein G affinity purified
Target Protein:Human Thr181 phosphorylated Tau
Immunogen:KLH conjugated with a short peptide [Ala-Pro-Lys-Thr(p)-Pro-Pro-Ser-Ser-Gly-Glu-Cys], corresponding to the amino acid sequence 177-187 on the phosphorylated Tau protein.
Fusion Myeloma:Sp2/0-Ag14
Specificity:This antibody reacts with the unconjugated Thr181 phosphorylated Tau peptide.
Species Reacitvity:Human, mouse
Host / Isotype: Mouse, IgG2a Kappa
Formulation:Lyophilized from a solution in 0.01M PBS pH7.2
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL. Avoid repeated freeze and thaw cycle.
Storage: Store at -20oC
Research Area:Alzheimer’s disease and other neurodegenerative diseases
Background:
Tau is a highly soluble microtubule associated protein found in high concentration in neuron of central nervous system. The main function of the protein is stabilizing the microtubule in nerve fibre (axon). There are six tau isoforms which are formed by alternate splicing. Hyperphosphorylation of Tau protein results in aggregation and formation of neurofibrillary tangles, which is significantly associated with cognitive impairment in Alzheimer’s disease. Tau Thr181 phosphorylation has been used as a biomarker for the differentiation of Alzheimer’s disease and other primary dementia such as dementia with Lewy bodies..
Application:
1.Indirect ELISA: The antibody reacts with phosphorylated peptide [Ala-Pro-Lys-Thr(p)-Pro-Pro-Ser-Ser-Gly-Glu-Cys] coated ELISA plate in indirect ELISA. Cross-reactivity with un-phosphorylated peptide is very low.
2. Western Blot: The antibody detected a 46kD band in mouse brain tissue lysate.
Detect the mouse brain tissue lysates using antibody (Tau-pho181 7G9) at 1:2000 (0.5 μg/mL) dilution.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-Thr181 Phosphorylated Tau, 7G9 |
|---|---|
| Application | EIA, WB |
| Size | 0.1 mg |
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② 应取pH8.0，这样可使核苷酸带较多负电荷，利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷，但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时，根据核苷酸负电荷的多少来决定洗脱速度，则洗脱顺序为CMP>AMP> GMP > UMP，但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附，嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为：CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大，甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高，也是一个原因
我使用的是1%琼脂糖凝胶，电泳缓冲液为0.5×TBE。
请问各位大虾，在不影响实验结果的前提下，这样的含EB的电泳缓冲液可以反复使用多少次？
谢谢^_^
500ml
2。阴极缓冲液 ( 10 × ) ：将 60.55g Tris ,89.58g Tricine 及 5g SDS 溶于400ml 蒸馏水中，加水至终体积为500ml
使用时稀释至1×的缓冲液，电泳槽内槽加入阴极电泳缓冲液，外槽加入阳极电泳缓冲液。形成Trcine-SDS-PAGE电泳系统。
如果你是制胶或者做电泳缓冲液的话，不用灭菌。
Tris-Glycine/SDS;
MOPS/SDS;
Bis-Glycine/SDS等不同的缓冲液、预混液等。
不知道这几种缓冲液有什么异同呢？
比如如果是Invitrogen的预制胶-预混液电泳系统，每种预制胶有适配缓冲液，但是如果用其他的缓冲液似乎也可以得到不错的结果，那么是否说明电泳缓冲液只要满足了缓冲ph范围，其他的不是特别重要呢？
此外，附加提问是，PVDF膜在转膜的时候，转膜缓冲液中不需要加入甲醇，那么是加入甲醇转膜效果好还是不加好呢？
谢谢！
