The reticular fiber silver stain is commonly used to demonstrate reticular fibers. The method developed by Gordon and Sweet made further improvement on the reticulin silver stain. However, these reticulin staining procedures use relatively large volume of staining solutions, are costly, time-consuming, and difficult to achieve consistent results.Hito Reticulin OptimStain™ PAP-Pen Kit offers a simple solution to these problems. This kit is easy to use with simplified procedures. The users can process slides in small quantities of solutions . The staining step can be controlled slide by slide to achieve optimal staining and differentiation for each slide. This kit delivers stable and improved staining quality, with minimal overstains, background and artifacts when used properly.Hito Reticulin OptimStain™ PAP-Pen Kit has been tested on the liver, spleen, pancreas, lung and other tissues from several species of animals and proven to be sensitive for demonstrating the morphological details of reticular fibers. It is a simple solution for your research.
| Kit Contents | for 50 Slides |
| Solution-1 | 20 ml |
| Solution-2 | 20 ml |
| Solution-3 | 20 ml |
| Solution-4A | 10 ml |
| Solution-4B | 10 ml |
| Solution-4C | 10 ml |
| Solution-5 | 20 ml |
| Solution-6 | 20 ml |
| Solution-7 | 20 ml |
| Solution-8 | 20 ml |
| Hito Aqua Barrier PAP Pen | 1 |
| User Manual and MSDS | 1 |
Before using Hito Reticulin OptimStain™ PAP-Pen Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Cryostat or Microtome, Light microscopeParaffin embedding equipment (or paraffin sections)Hito Bouin’s Plus Solution (Cat# HTSH0102, for paraffin sections preparation)Dry ice, isopentane, O.C.T. compound (for frozen sections), 4% PFA (Cat# HTSH0101), ethanol, xylene, double distilled or deionized waterSlides, coverslipsStaining jars for slides washResinous mounting medium  Hito Reticulin OptimStain™ PAP-Pen Kit Manual and MSDS | |
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我使用的是1%琼脂糖凝胶，电泳缓冲液为0.5×TBE。
请问各位大虾，在不影响实验结果的前提下，这样的含EB的电泳缓冲液可以反复使用多少次？
谢谢^_^
此外，我们常常用“电泳法”判断液体的性质，是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转
2.配好的EDTA不灭菌行不?放4度冰箱等过完年回来直接拿去配TAE
3.TAE配好后不用灭菌吧
TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量（TBE中电泳时测出的相对分子质量会大于实际分子质量），且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%，电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果，此外，回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小，长时间电泳（如过夜）不可选用，除非有循环装置使两极的缓冲液得到交换。 50×TAE Buffer 配制方法： 1. 称量Tris 242g，Na2EDTA.2H2O 37.2g 于1L烧杯中； 2.向烧杯中加入约800ml去离子水，充分搅拌均匀； 3加入57.1ml的冰乙酸，充分溶解； 4.加去离子水定容至1L后，室温保存。向左转|向右转
此外，我们常常用“电泳法”判断液体的性质，是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
加样缓冲液的主要作用是使PCR产物与其混合,使DNA沉于加样孔的底部,防止DNA跑出来.
