- SpeciesReactivityMouse
- SpecificityDetectsmouseNKG2DindirectELISAsandWesternblots.Doesnotcross-reactwithrecombinanthumanNKG2D.
- SourceMonoclonalRatIgG2BClone#191004
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenMousemyelomacelllineNS0-derivedrecombinantmouseNKG2D
Phe94-Val232
Accession#O54709 - FormulationSuppliedinasalinesolutioncontainingBSAandSodiumAzide.
- LabelPhycoerythrin
- FlowCytometry10µL/106cellsSeebelow
- ShippingTheproductisshippedwithpolarpacks.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.
- StABIlity&StorageProtectfromlight.Donotfreeze.
- 12monthsfromdateofreceipt,2to8°Cassupplied.
- Bauer,S.etal.(1999)Science285:727.
- Wu,J.etal.(1999)Science285:730.
- Diefenbach,A.etal.(2001)Nature413:165.
- Vivier,E.etal.(2002)Curr.Opin.Immunol.14:306.
- NKG2DanditsLigands;www.RnDSystems.com.
- LongName:NaturalKillerG2D
- EntrezGeneIDs:22914(Human);27007(Mouse)
- AlternateNames:CD314antigen;CD314;D12S2489E;FLJ17759;FLJ75772;Killercelllectin-likereceptorsubfamilyKmember1;killercelllectin-likereceptorsubfamilyK,member1;KLR;KLRK1;NKcellreceptorD;NKG2-DtypeIIintegralmembraneprotein;NKG2D;NKG2-D;NKG2-D-activatingNKreceptor;NKG2DDNAsegmentonchromosome12(unique)2489expressedsequence
Background:
NKG2DisatypeIItransmembraneproteinwithanextracellularC-typelectin-likedomain.Itoccursasadisulfide-linkedhomodimerthatassociateswiththetransmembraneDAP10(DNAX-activatorprotein10)adapterproteintodeliveranactivatingsignal.Thisproteinsharesapproximately25%aminoacidsequenceidentitywithanumberofothertypeIIlectin-likeproteinsthatareencodedbygeneswithinthenaturalkillercomplexonmousechromosome6.NKG2DisexpressedonNKcells,whereitfunctionsasanactivatingreceptortotriggercytolyticactivityandcytokinesecretion,andonsomeTcellsubsets,whereitactsasaco‑stimulatoryreceptorcomplementingTcellreceptorsignaling.SeveralligandshavenowbeenidentifiedformouseNKG2DincludingH60andRae1alpha,beta,gamma,δ,andepsilon.Alloftheseligandsarecell-surfaceproteinsdistantlyrelatedtoMHCclassI.However,theydonotbindpeptideorassociatewithbeta2-microglobulin.Ligandexpressionisup-regulatedinmanytransformedcelllinesandalsoduringconditionsofstresssuchasheatshockorviralinfection.Invivo,tumormodelsdemonstratethatNKG2Dfunctionsinanti-tumorsurveillance(1-5).
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② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

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