
- SpeciesReactivityMouse
- SpecificityDetectsmouseNKG2DindirectELISAsandWesternblots.Doesnotcross-reactwithrecombinanthumanNKG2D.
- SourceMonoclonalRatIgG2BClone#191004
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenMousemyelomacelllineNS0-derivedrecombinantmouseNKG2D
Phe94-Val232
Accession#O54709 - FormulationSuppliedinasalinesolutioncontainingBSAandSodiumAzide.
- LabelPhycoerythrin
- FlowCytometry10µL/106cellsSeebelow
- ShippingTheproductisshippedwithpolarpacks.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.
- StABIlity&StorageProtectfromlight.Donotfreeze.
- 12monthsfromdateofreceipt,2to8°Cassupplied.
- Bauer,S.etal.(1999)Science285:727.
- Wu,J.etal.(1999)Science285:730.
- Diefenbach,A.etal.(2001)Nature413:165.
- Vivier,E.etal.(2002)Curr.Opin.Immunol.14:306.
- NKG2DanditsLigands;www.RnDSystems.com.
- LongName:NaturalKillerG2D
- EntrezGeneIDs:22914(Human);27007(Mouse)
- AlternateNames:CD314antigen;CD314;D12S2489E;FLJ17759;FLJ75772;Killercelllectin-likereceptorsubfamilyKmember1;killercelllectin-likereceptorsubfamilyK,member1;KLR;KLRK1;NKcellreceptorD;NKG2-DtypeIIintegralmembraneprotein;NKG2D;NKG2-D;NKG2-D-activatingNKreceptor;NKG2DDNAsegmentonchromosome12(unique)2489expressedsequence
Background:
NKG2DisatypeIItransmembraneproteinwithanextracellularC-typelectin-likedomain.Itoccursasadisulfide-linkedhomodimerthatassociateswiththetransmembraneDAP10(DNAX-activatorprotein10)adapterproteintodeliveranactivatingsignal.Thisproteinsharesapproximately25%aminoacidsequenceidentitywithanumberofothertypeIIlectin-likeproteinsthatareencodedbygeneswithinthenaturalkillercomplexonmousechromosome6.NKG2DisexpressedonNKcells,whereitfunctionsasanactivatingreceptortotriggercytolyticactivityandcytokinesecretion,andonsomeTcellsubsets,whereitactsasaco‑stimulatoryreceptorcomplementingTcellreceptorsignaling.SeveralligandshavenowbeenidentifiedformouseNKG2DincludingH60andRae1alpha,beta,gamma,δ,andepsilon.Alloftheseligandsarecell-surfaceproteinsdistantlyrelatedtoMHCclassI.However,theydonotbindpeptideorassociatewithbeta2-microglobulin.Ligandexpressionisup-regulatedinmanytransformedcelllinesandalsoduringconditionsofstresssuchasheatshockorviralinfection.Invivo,tumormodelsdemonstratethatNKG2Dfunctionsinanti-tumorsurveillance(1-5).
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TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量(TBE中电泳时测出的相对分子质量会大于实际分子质量),且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%,电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果,此外,回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小,长时间电泳(如过夜)不可选用,除非有循环装置使两极的缓冲液得到交换。 50×TAE Buffer 配制方法: 1. 称量Tris 242g,Na2EDTA.2H2O 37.2g 于1L烧杯中; 2.向烧杯中加入约800ml去离子水,充分搅拌均匀; 3加入57.1ml的冰乙酸,充分溶解; 4.加去离子水定容至1L后,室温保存。向左转|向右转
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。


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