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A 1:1000 dilution of SPC-1274 was sufficient for detection of Collagen II in 10 µg of COLO205 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Biological Description
Alternative Names
Alpha 1 type II collagen Antibody, AOM Antibody, Cartilage collagen Antibody, Chondrocalcin Antibody, COL11A3 Antibody, Collagen II alpha 1 polypeptide Antibody, SEDC Antibody
Type-II collagen is the less abundant form of collagen in the human body and can be found in articular and hyaline cartilages and the eyes. It has been associated with the treatment of arthritis, cellulite and wrinkles.
References
1. Mullazehi M., et al. (2012) Arthritis Research Ther. 14:R100
Product Images
Immunohistochemistry analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide.
Western blot analysis of Human COLO205 cell lysates showing detection of ~142kDa Collagen II protein using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Lane 1: Human COLO205. Lane 2: Human COLO205 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:1000. Predicted/Observed Size: ~142kDa.
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Biotin
Properties:
Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C10H16N2O3S
Applications: Western blot, immunohistochemistry, and ELISA
① 电泳分离4种核苷酸时应取pH3.5 的缓冲液,在该pH时,这4种单核苷酸之间所带负电荷差异较大,它们都向正极移动,但移动的速度不同,依次为:UMP>GMP>AMP>CMP; ② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。 ③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。