Overview:
| Product Name | Cyclooxygenase 1 Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Description | Rabbit Anti-Human Cyclooxygenase 1 Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Applications | WB, ICC/IF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Antibody Dilution | WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunogen | Synthetic peptide of Human Cyclooxygenase | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet Biotin | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| R-PE (R-Phycoerythrin) | ||
Overview:
R-PE Datasheet | ![]() | Optical Properties: λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
Properties
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
| Storage Temperature | -20ºC |
| Shipping Temperature | Blue Ice or 4ºC |
| Purification | Peptide Affinity Purified |
| Clonality | Polyclonal |
| Specificity | Detects ~75 kDa. |
| Cite This Product | StressMarq Biosciences Cat# SPC-707, RRID: AB_2705987 |
| Certificate of Analysis | A 1:1000 dilution of SPC-707 was sufficient for detection of Cyclooxygenase in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Biological Description
| Alternative Names | EC:1.14.99.1 Antibody, PGHS1 Antibody, Prostaglandin G/H synthase 1 Antibody, PGHS-1 Antibody, Prostaglandin H2 synthase 1 Antibody, Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) Antibody, PHS 1 Antibody, EC 1.14.99.1 Antibody, Prostaglandin-endoperoxide synthase 1 Antibody, COX 3 Antibody, PTGS1 Antibody, PGH1_HUMAN Antibody, COX-1 Antibody, PGG/HS Antibody, PTGHS Antibody, Cox3 Antibody, PGH synthase 1 Antibody, Cyclooxygenase-1 Antibody, PHS1 Antibody, Cyclooxygenase 1 Antibody, Cyclooxygenase 3, included Antibody, Partial COX1 proteins, included Antibody, COX 1 Antibody, COX1 Antibody, PCOX1 Antibody |
| Research Areas | Cancer, Atherosclerosis, Blood, Cancer Lipid and Lipoprotein Metabolism, Cancer Metabolism, Cardiovascular System, Cell Signaling, Immunology, Innate Immunity, Lipid and lipoprotein Metabolism, Macrophage, Metabolism, Metabolism and Oxidative Stress, Platelets, Redox Metabolism |
| Cellular Localization | Endoplasmic reticulum membrane, Microsome Membrane, Peripheral membrane protein |
| Accession Number | NP_000953.2 |
| Gene ID | 5742 |
| Swiss Prot | P23219 |
Product Images
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.
Western blot analysis of Mouse Kidney cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.
Western blot analysis of Rat Brain cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.
Product Citations (0)
Currently there are no citations for this product.
| ATTO 488 | ||
Overview:
ATTO 488 Datasheet | ![]() | Optical Properties: λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
500ml
2。阴极缓冲液 ( 10 × ) :将 60.55g Tris ,89.58g Tricine 及 5g SDS 溶于400ml 蒸馏水中,加水至终体积为500ml
使用时稀释至1×的缓冲液,电泳槽内槽加入阴极电泳缓冲液,外槽加入阳极电泳缓冲液。形成Trcine-SDS-PAGE电泳系统。
如果你是制胶或者做电泳缓冲液的话,不用灭菌。
Tris-Glycine/SDS;
MOPS/SDS;
Bis-Glycine/SDS等不同的缓冲液、预混液等。
不知道这几种缓冲液有什么异同呢?
比如如果是Invitrogen的预制胶-预混液电泳系统,每种预制胶有适配缓冲液,但是如果用其他的缓冲液似乎也可以得到不错的结果,那么是否说明电泳缓冲液只要满足了缓冲ph范围,其他的不是特别重要呢?
此外,附加提问是,PVDF膜在转膜的时候,转膜缓冲液中不需要加入甲醇,那么是加入甲醇转膜效果好还是不加好呢?
谢谢!















