
Overview:
Product Name | DDX4 Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Rabbit Anti-Human DDX4 Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Peptide sequence around aa.273~277(T-I-L-V-E)derived from Human DDX4. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Affinity Purified |
Clonality | Polyclonal |
Isotype | IgG |
Specificity | Detects endogenous level of total DDX4 protein. |
Cite This Product | StressMarq Biosciences Cat# SPC-1322, RRID: AB_2713583 |
Certificate of Analysis | A 1:1000 dilution of SPC-1322 was sufficient for detection of DDX4 in 10 µg of HeLa, K562, 3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | DDX 4 antibody, Ddx4 antibody, DDX4_HUMAN antibody, DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody, DEAD box protein 4 antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody, MVH antibody, Probable ATP dependent RNA helicase DDX4 antibody, Probable ATP-dependent RNA helicase DDX4 antibody, VASA antibody, Vasa homolog antibody |
Cellular Localization | Cytoplasm, Perinuclear Region |
Accession Number | NP_077726.1 |
Gene ID | 54514 |
Swiss Prot | Q9NQI0 |
Scientific Background | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. |
References |
1. Castrillon D.H. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97:9585-9590. 2. Schmutz J. et al. (2004) Nature 431:268-274. |
Product Images

Western blot analysis of Human, Mouse HeLa, K562, 3T3 cell lysates showing detection of ~80kDa DDX4 protein using Rabbit Anti-DDX4 Polyclonal Antibody (SPC-1322). Lane 1: Human HeLa. Lane 2: Human K562. Lane 3: Mouse 3T3. Primary Antibody: Rabbit Anti-DDX4 Polyclonal Antibody (SPC-1322) at 1:1000. Predicted/Observed Size: ~80kDa.
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但是最好还是不要用,影响实验就不好了。
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

