![StressMarq/MOLECULAR SIGNATURE® Anti-Dibromo-tyrosine Antibody [9F12]/SMC-507D-A594/100-µg](images/StressMarq/201710/SMC-507_Dibromo-tyrosine_Antibody_9F12_ICC-IF_Human_Embryonic-kidney-cells-HEK293_Composite_1-150x150.png)
Overview:
Product Name | Dibromo-tyrosine Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Mouse Anti-Dibromo-tyrosine (DiBrY) Monoclonal IgG1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Species Independent | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, ICC/IF, ELISA | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Synthetic 3,5-Dibromotyrosine conjugated to Keyhole Limpet Kemocyanin (KLH). | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | 9F12 |
Isotype | IgG1 |
Specificity | Specific for 3,5-Dibromo-tyrosine. Does not cross-react with Nitrotyrosine. |
Cite This Product | StressMarq Biosciences Cat# SMC-507, RRID: AB_2702765 |
Certificate of Analysis | A 1:1000 dilution of SMC-507 was sufficient for detection of 3,5-Dibromotyrosine in 1 µg of 3, 5-Dibromotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. |
Biological Description
Alternative Names | 3,5-Dibromo-tyrosine Antibody, 3,5-dibromotyrosine Antibody, Dibromotyrosine Antibody, Dibromo-tyrosine Antibody, DiBrY Antibody, Dibromotyrosine (DiBrY) Antibody, Dibromo-Tyrosine (DiBrY) Antibody, Dibromo-tyrosine [DiBrY] Antibody |
Research Areas | Cancer, Cell Signaling, Oxidation, Oxidative Stress, Post-translational Modifications, Protein Oxidation |
Product Images

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12 (SMC-507). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody (SMC-507) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) – Untreated. (B,D,F,H) – Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dibromo-tyrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Western Blot analysis of 3, 5-Dibromotyrosine-BSA Conjugate showing detection of 67 kDa 3,5-Dibromotyrosine-BSA using Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody, Clone 9F12 (SMC-507). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: Nitrosylated-BSA. Lane 4: Dibromotyrosine-BSA. Load: 1 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody (SMC-507) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of 3,5-Dibromotyrosine-BSA using Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody, Clone 9F12 (SMC-507). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody (SMC-507) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
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ATTO 594 | ||
Overview:
ATTO 594 Datasheet | ![]() | Optical Properties: λex = 601 nm λem = 627 nm εmax = 1.2×105 Φf = 0.85 τfl = 3.5 ns Brightness = 102 Laser = 594 nm Filter set = Texas Red® |
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但是最好还是不要用,影响实验就不好了。
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

