A 1:250 dilution of SPC-1090 was sufficient for detection of TNK1 (pTyr277) in 10 µg of human K562 cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Biological Description
Alternative Names
CD38 negative kinase 1 Antibody, EC 2.7.10.2 Antibody, Kinase of embryonic stem cells Antibody, Kos 1 Antibody, Kos1 Antibody, MGC46193 Antibody, Non receptor tyrosine protein kinase TNK 1 Antibody, Non receptor tyrosine protein kinase TNK1 Antibody, Non-receptor tyrosine-protein kinase TNK1 Antibody, Tnk 1 Antibody, Tnk1 Antibody, TNK1_HUMAN Antibody, Tyrosine kinase non receptor 1 Antibody
Cellular Localization
Cytoplasm, Membrane
Accession Number
NP_003976
Gene ID
8711
Swiss Prot
Q13470
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Biotin
Properties:
Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C10H16N2O3S
Applications: Western blot, immunohistochemistry, and ELISA
① 电泳分离4种核苷酸时应取pH3.5 的缓冲液，在该pH时，这4种单核苷酸之间所带负电荷差异较大，它们都向正极移动，但移动的速度不同，依次为：UMP>GMP>AMP>CMP； ② 应取pH8.0，这样可使核苷酸带较多负电荷，利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷，但pH过高对分离不利。 ③ 当不考虑树脂的非极性吸附时，根据核苷酸负电荷的多少来决定洗脱速度，则洗脱顺序为CMP>AMP> GMP > UMP，但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附，嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为：CMP> AMP > UMP >GMP。
