Overview:
| Product Name | VDAC1 Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Description | Mouse Anti-Human VDAC1 Monoclonal IgG2a | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Applications | WB, IHC, ICC/IF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Antibody Dilution | WB (1:1000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Host Species | Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunogen | Fusion protein amino acids 1-283 (full-length) of human VDAC1. Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) >60% identity with VDAC2 and VDAC3. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet Biotin | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| R-PE (R-Phycoerythrin) | ||
Overview:
R-PE Datasheet | ![]() | Optical Properties: λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
Properties
| Storage Buffer | PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
| Storage Temperature | -20ºC |
| Shipping Temperature | Blue Ice or 4ºC |
| Purification | Protein G Purified |
| Clonality | Monoclonal |
| Clone Number | S152B-23 |
| Isotype | IgG2a |
| Specificity | Detects ~30kDa. Does not cross-react with VDAC2 or VDAC3 (based on KO validation results). |
| Cite This Product | StressMarq Biosciences Cat# SMC-456, RRID: AB_2701955 |
| Certificate of Analysis | 1 µg/ml of SMC-456 was sufficient for detection of VDAC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Biological Description
| Alternative Names | Voltage Dependent Anion Channel 1 Antibody, Porin Antibody, Voltage dependent anion selective channel protein 1 Antibody, Voltage-dependent anion-selective channel protein 1 Antibody, hVDAC1 Antibody, MGC111064 Antibody, Mitochondrial Porin Antibody, Outer mitochondrial membrane protein porin 1 Antibody, Plasmalemmal porin Antibody, Porin 31HL Antibody, Porin 31HM Antibody, PORIN-31-HL Antibody, VDAC 1 Antibody, VDAC Antibody, VDAC-1 Antibody |
| Research Areas | Cancer, Cell Signaling, Ion Channels, Neuroscience |
| Cellular Localization | Cell membrane, Mitochondrion, Mitochondrion outer membrane |
| Accession Number | NP_003365.1. |
| Gene ID | 7416 |
| Swiss Prot | P21796 |
| Scientific Background | Voltage-dependent anion-selective channel protein 1 (also known as VDAC, VDAC1 or outer mitochondrial membrane protein porin 1) is the the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. VDAC may participate in the formation of the permeability transition pore complex (PTPC), which is responsible for the release of mitochondrial products that triggers apoptosis. |
Product Images
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (SMC-456). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (SMC-456) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Outer Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) VDAC1 Antibody (D) Composite.
Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone S152B-23 (SMC-456). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody (SMC-456) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.
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| ATTO 565 | ||
Overview:
ATTO 565 Datasheet | ![]() | Optical Properties: λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
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② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
500ml
2。阴极缓冲液 ( 10 × ) :将 60.55g Tris ,89.58g Tricine 及 5g SDS 溶于400ml 蒸馏水中,加水至终体积为500ml
使用时稀释至1×的缓冲液,电泳槽内槽加入阴极电泳缓冲液,外槽加入阳极电泳缓冲液。形成Trcine-SDS-PAGE电泳系统。
如果你是制胶或者做电泳缓冲液的话,不用灭菌。
Tris-Glycine/SDS;
MOPS/SDS;
Bis-Glycine/SDS等不同的缓冲液、预混液等。
不知道这几种缓冲液有什么异同呢?
比如如果是Invitrogen的预制胶-预混液电泳系统,每种预制胶有适配缓冲液,但是如果用其他的缓冲液似乎也可以得到不错的结果,那么是否说明电泳缓冲液只要满足了缓冲ph范围,其他的不是特别重要呢?
此外,附加提问是,PVDF膜在转膜的时候,转膜缓冲液中不需要加入甲醇,那么是加入甲醇转膜效果好还是不加好呢?
谢谢!















