
Overview:
Product Name | VE Cadherin Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Rabbit Anti-Human VE Cadherin Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:2000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | A synthesized peptide derived from human VE-Cadherin | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Affinity Purified |
Clonality | Polyclonal |
Isotype | IgG |
Specificity | Detects endogenous levels of total VE-Cadherin |
Cite This Product | StressMarq Biosciences Cat# SPC-1290, RRID: AB_2713025 |
Certificate of Analysis | A 1:1000 dilution of SPC-1290 was sufficient for detection of VE Cadherin in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | 7B4 Antibody, Cadherin 5 Antibody, Cadherin 5 type 2 Antibody, cadherin, vascular endothelial Antibody, CD 144 Antibody, CDH 5 Antibody, Endothelial specific cadherin Antibody, Vascular endothelial cadherin Antibody, VE Cad Antibody |
Research Areas | Cancer, Cardiovascular Markers, Cardiovascular System, Cell adhesion, Cell Signaling, Cytoskeleton, Endothelial Cells, Invasion/microenvironment |
Cellular Localization | Cell Junction, Cell membrane |
Accession Number | NP_001786.2 |
Gene ID | 1003 |
Swiss Prot | P33151 |
Scientific Background | Cadherins are a super family of transmembrane glycoproteins that contain cadherin repeats of ~ 100 residues in their extracellular domain. They mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. Specifically, VE Cadherin plays an inportant role in endothelial cell biology. It associates with alpha-catenin forming a link to the cytoskeleton. And its signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis. |
References |
1. Wheelock M.J. and Johnson K.R. (2003) Annu Rev Cell Dev Biol. 19: 207-235. 2. Rabascio C., et al. (2004) Cancer Res. 64: 4373-4377. 3. Yamaoka-Tojo M., et al. (2006) Arterioscler Thromb Vasc Biol. 26: 1991-1997. |
Product Images

Western blot analysis of Human Jurkat cell lysates showing detection of ~120kDa VE Cadherin protein using Rabbit Anti-VE Cadherin Polyclonal Antibody (SPC-1290). Lane 1: Human Jurkat Whole Cell lysates treated with the immunizing peptide. Lane 2: Human Jurkat Whole Cell lysates. Primary Antibody: Rabbit Anti-VE Cadherin Polyclonal Antibody (SPC-1290) at 1:1000. Predicted/Observed Size: ~120kDa.
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但是最好还是不要用,影响实验就不好了。
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

