+,StressMarq/Anti-VE Cadherin Antibody/SPC-1290D-A390/100-µl,电泳缓冲液,StressMarq,StressMarq,,atto-390,100-µl蚂蚁淘商城

商品信息

联系客服

StressMarq/Anti-VE Cadherin Antibody/SPC-1290D-A390/100-µl

郑重提醒：

无质量问题不接受退换货，下单前请仔细核对信息。

下单后请及时联系客服核对商品价格，订单生效后再付款。

StressMarq/Anti-VE Cadherin Antibody/SPC-1290D-A390/100-µl

品牌 /

StressMarq

货号 /

SPC-1290D-A390

官网地址

美元价：

登录查看

(友情提示：该价格仅为参考，欢迎联系客服询价！)

数量：

免费咨询热线

4000-520-616

商品介绍售后保障相关文章商品咨询

联系客服

Overview:

Product Name VE Cadherin Antibody

Description

Rabbit Anti-Human VE Cadherin Polyclonal

Species Reactivity Human, Mouse

Applications WB

Antibody Dilution WB (1:2000); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Human

Immunogen A synthesized peptide derived from human VE-Cadherin

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex
Small phycobiliprotein
Isolated from red algae
Large stokes shift (195 nm)
Molecular Weight: 35 kDa

PerCP Datasheet

PerCP Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser = 488 nm

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation

PE/ATTO 594 Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

Optical Properties:

λ_ex = 535 nm

λ_em = 627 nm

Laser = 488 to 561 nm

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield
High rate of photobleaching
Good solubility in water
Broad emission spectrum
pH dependent spectra
Molecular formula: C₂₀H₁₂O₅
Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 494 nm

λ_em = 520 nm

ε_max = 7.3×10⁴

Φ_f = 0.92

τ_fl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

ATTO 700

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 575 g/mol

ATTO 700 Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 700 nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f = 0.25

τ_fl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

ATTO 680

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 631 g/mol

ATTO 680 Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 680 nm

λ_em = 700 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

ATTO 655

Overview:

High fluorescence yield
High thermal and photostability
Excellent ozone resistance
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 634 g/mol

ATTO 655 Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

ATTO 633

Overview:

High fluorescence yield
High thermal and photostability
Moderately hydrophilic
Good solubility in polar solvents
Stable at pH 4 – 11
Cationic dye, perchlorate salt
Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

ATTO 594

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 1137 g/mol

ATTO 594 Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

ATTO 565

Overview:

High fluorescence yield
High thermal and photostability
Good solubility in polar solvents
Excellent solubility in water
Very little aggregation
Rhodamine dye derivative
Molar Mass: 611 g/mol

ATTO 565 Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

ATTO 488

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 804 g/mol

ATTO 488 Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

ATTO 390

Overview:

High fluorescence yield
Large Stokes-shift (89 nm)
Good photostability
Moderately hydrophilic
Good solubility in polar solvents
Coumarin derivate, uncharged
Low molar mass: 343.42 g/mol

ATTO 390 Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

APC (Allophycocyanin)

Overview:

High quantum yield
Large phycobiliprotein
6 chromophores per molecule
Isolated from red algae
Molecular Weight: 105 kDa

APC Datasheet

APC Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
Molecular weight: 53 kDa
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target
Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors
Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
High turnover rate enables rapid generation of a strong signal
44 kDa glycoprotein
Extinction coefficient: 100 (403 nm)
Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
Molecular weight: 140 kDa
Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum
High quantum yield
Photostable
Member of the phycobiliprotein family
Isolated from red algae
Excellent solubility in water
Molecular Weight: 250 kDa

R-PE Datasheet

R-PE Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f = 0.84

Brightness = 1.68 x 10³

Laser = 488 to 561 nm

Filter set = TRITC

Properties

Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Affinity Purified
Clonality	Polyclonal
Isotype	IgG
Specificity	Detects endogenous levels of total VE-Cadherin
Cite This Product	StressMarq Biosciences Cat# SPC-1290, RRID: AB_2713025
Certificate of Analysis	A 1:1000 dilution of SPC-1290 was sufficient for detection of VE Cadherin in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	7B4 Antibody, Cadherin 5 Antibody, Cadherin 5 type 2 Antibody, cadherin, vascular endothelial Antibody, CD 144 Antibody, CDH 5 Antibody, Endothelial specific cadherin Antibody, Vascular endothelial cadherin Antibody, VE Cad Antibody
Research Areas	Cancer, Cardiovascular Markers, Cardiovascular System, Cell adhesion, Cell Signaling, Cytoskeleton, Endothelial Cells, Invasion/microenvironment
Cellular Localization	Cell Junction, Cell membrane
Accession Number	NP_001786.2
Gene ID	1003
Swiss Prot	P33151
Scientific Background	Cadherins are a super family of transmembrane glycoproteins that contain cadherin repeats of ~ 100 residues in their extracellular domain. They mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. Specifically, VE Cadherin plays an inportant role in endothelial cell biology. It associates with alpha-catenin forming a link to the cytoskeleton. And its signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis.
References	1. Wheelock M.J. and Johnson K.R. (2003) Annu Rev Cell Dev Biol. 19: 207-235. 2. Rabascio C., et al. (2004) Cancer Res. 64: 4373-4377. 3. Yamaoka-Tojo M., et al. (2006) Arterioscler Thromb Vasc Biol. 26: 1991-1997.

Product Images

<p>Western blot analysis of Human Jurkat cell lysates showing detection of ~120kDa VE Cadherin protein using Rabbit Anti-VE Cadherin Polyclonal Antibody (SPC-1290). Lane 1: Human Jurkat Whole Cell lysates treated with the immunizing peptide. Lane 2: Human Jurkat Whole Cell lysates. Primary Antibody: Rabbit Anti-VE Cadherin Polyclonal Antibody (SPC-1290) at 1:1000. Predicted/Observed Size: ~120kDa.</p>

Western blot analysis of Human Jurkat cell lysates showing detection of ~120kDa VE Cadherin protein using Rabbit Anti-VE Cadherin Polyclonal Antibody (SPC-1290). Lane 1: Human Jurkat Whole Cell lysates treated with the immunizing peptide. Lane 2: Human Jurkat Whole Cell lysates. Primary Antibody: Rabbit Anti-VE Cadherin Polyclonal Antibody (SPC-1290) at 1:1000. Predicted/Observed Size: ~120kDa.

Product Citations (0)

Currently there are no citations for this product.

ATTO 390

Overview:

High fluorescence yield
Large Stokes-shift (89 nm)
Good photostability
Moderately hydrophilic
Good solubility in polar solvents
Coumarin derivate, uncharged
Low molar mass: 343.42 g/mol

ATTO 390 Datasheet

Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

蚂蚁淘电商平台
ebiomall.com

公司简介

蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...

蚂蚁淘承诺

正品保证： 全球直采在线追溯蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权；同时客户可以通过订单详情查看到货物从厂方至客户的所有流程，确保货物的来源；正规报关，提供13%增值税发票。

及时交付： 限时必达畅选无忧蚂蚁淘的运营团队都是有着多年经验的成员，他们熟悉海外采购、仓储物流、报关等环节；同时通过在线的流程监控，蚂蚁淘的进口速度比传统企业提高了50%以上，部分产品甚至能做到7-10天到货，即蚂蚁淘的“时必达”服务。

轻松采购： 在线下单简单省事蚂蚁淘的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价；报价单与合同可以直接在线生成或打印；就像在京东购物一样，您的鼠标点击几次即完成在蚂蚁淘的采购，订单详情会告诉您所有进程。

售后申请： 耐心讲解优质服务蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时，您可通过我的订单提交您的“申请售后”，蚂蚁淘产品顾问会第一时间为您处理，在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。

KINGREPA TRADING (S) PTE LTD | 商业公司详细资料

2021-07-26

北京华夏远洋科技有限公司在发布的三氟乙酸钯(II)，Palladium(II) trifluoroacetate，CAS号：42196-31-6，分子式：Pd(O2CCF3)2，98%供应信息，浏览与三氟乙酸钯(II)，Palladium(II) trifluoroacetate，CAS号：42196-31-6，分子式：Pd(O2CCF3)2，98%相关的产品或在搜索更多与三氟乙酸钯(II)，Palladium(II) trifluoroacetate，CAS号：42196-31-6，分子式：Pd(O2C 查看更多>

足迹法学术百科知网空间

2018-12-19

KRPH缓冲液（500ml）无菌溶液现配现用！中文名称：KRPH缓冲液规格：500ml用途：Sigma-MAK083 KREBS-RIN 查看更多>

Randi Group | náhradní plnění

2021-08-12

上海研生实业有限公司所提供的吲哚-3-乙酸抗,植物生长素抗体质量可靠、规格齐全,上海研生实业有限公司不仅具有精湛的技术水平,更有良好的售后服务和优质的解决方案,欢迎您来电咨询此产品具体参数及价格等详细信息！查看更多>

《辉煌中国》第二集丨创新活力

2021-08-01

Tris-醋酸-SDS电泳缓冲液是由上海联硕生物科技有限公司代理或销售的Amresco品牌的试剂，产品来源于美国。上海联硕生物科技有限公司是中国最权威的Tris-醋酸-SDS电泳缓冲液试剂销售服务商之一，在上海等地方销售Tris-醋酸-SDS电泳缓冲液试剂已经多年。生物在线为您提供众多企业Tris-醋酸-SDS电泳缓冲液仪器产品及图片，以便挑选到性价比高，合适的Tris-醋酸-SDS电泳缓冲液产品查看更多>

AntiCD86/B72 抗体, CD86抗体促销供应

2021-09-22

3M 乙酸钠(pH5.2)是由北京索莱宝科技有限公司代理或销售的solarbio品牌的试剂，产品来源于北京市通州区马驹桥联东U谷86A。北京索莱宝科技有限公司是中国最权威的3M 乙酸钠(pH5.2)试剂销售服务商之一，在北京等地方销售3M 乙酸钠(pH5.2)试剂已经多年。生物在线为您提供众多企业3M 乙酸钠(pH5.2)仪器产品及图片，以便挑选到性价比高，合适的3M 乙酸钠(pH5.2)产品查看更多>

PP BC03GS 日本化聚原产资料

2021-09-12

上海宝曼生物科技有限公司在发布的草酰乙酸脱羧酶供应信息，浏览与草酰乙酸脱羧酶相关的产品或在搜索更多与草酰乙酸脱羧酶相关的内容。查看更多>

小鼠5羟色胺(5HT)酶联免疫分析(ELISA) 试剂盒使用说明书_产品说明_仪...

2020-08-01

上海沪震实业有限公司在发布的pH6.8磷酸盐缓冲液供应信息，浏览与pH6.8磷酸盐缓冲液相关的产品或在搜索更多与pH6.8磷酸盐缓冲液相关的内容。查看更多>

—美英关注食品添加糖标准科学报官微

2018-03-20

赫澎（上海）生物科技有限公司在发布的乙酸酐标准溶液供应信息，浏览与乙酸酐标准溶液相关的产品或在搜索更多与乙酸酐标准溶液相关的内容。查看更多>

人Toll样受体9(TLR9/CD289)elisa试剂盒

2018-07-18

MES-SDS电泳缓冲液产品名称：MES-SDS电泳缓冲液产品简介：上海通善生物是MES-SDS电泳缓冲液最权威的供应商，提供报价，咨询，技术服务，欢迎来电021-61806666咨询选购。产品库存：现货。产品价格：询价。供应商：通善生物。供应商地址：上海市闵行区金平路788弄166号。MES-SDS电泳缓冲液说明书：MES-SDS电泳缓冲液其它信息：货号产品名称级别K610-1L0.1X SSC BUFFER WITH 0.2% 查看更多>

敲除载体构建

2018-12-26

脉冲场凝胶电泳1）高分子量DNA琼脂糖凝胶块制备和加工1．收集10ml全血，加入30ml细胞裂解液。置冰浴中至少20min直至红细胞完全溶解。2．2000r/min离心10min，移去红色上清液，再次用细胞裂解液洗涤细胞，然后用PBS重悬细胞。3．稀释单细胞悬液并取一小份用Neubauer腔计数细胞。4．用PBS重悬查看更多>

Osaka Synthetic Chemical Lab进口数据及联系方式

2018-10-08

青岛高科技工业园海博生物技术有限公司在发布的PH6.8磷酸盐缓冲液供应信息，浏览与PH6.8磷酸盐缓冲液相关的产品或在搜索更多与PH6.8磷酸盐缓冲液相关的内容。查看更多>

酶标板洗板机,酶标板洗板机特点,全自动酶标板洗板机上海沛欧分析...

2019-06-20

北京绿源伯德生物科技有限公司在发布的北京现货-NA0034-500-Tris-硼酸电泳缓冲液(5×TBE，ph8.3)供应信息，浏览与北京现货-NA0034-500-Tris-硼酸电泳缓冲液(5×TBE，ph8.3)相关的产品或在搜索更多与北京现货-NA0034-500-Tris-硼酸电泳缓冲液(5×TBE，ph8.3)相关的内容。查看更多>

常见问题

蚂蚁淘所售产品均为正品吗？

蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑； Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。

下单后可以修改订单吗？

未确认状态的订单可以修改，打开“订单详情”页面，点击右上角的“修改订单”即可，若已审核确定，则订单无法修改。

商品几天可以发货？

现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。

订单如何取消？

如订单处于未确定状态，进入“我的订单"页面，找到要取消的订单，点击“取消订单”按钮。

可以开发票吗？

本网站所售商品都是正规清关，均开具13%正规发票，发票金额含配送费金额，另有说明的除外。

如何联系商家？

蚂蚁淘任何页面都有在线咨询功能，点击“联系客服”、“咨询”或“在线咨询”按钮，均可咨询蚂蚁淘在线客服人员，或拨打4000-520-616，除此之外客户可在联系我们页面找到更多的联系方式。

收到的商品少了/发错了怎么办？

同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

退换货/维修需要多长时间？

一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

商品咨询

SDSPAGE电泳相关溶液的配置123

qinhuan04252021-08-01

最近刚开始跑SDS-PAGE，很奇怪，加样后，Marker和样本停在加样孔不往下面跑！且跑电泳时，电压（80V）一直上不去，后测电泳液PH值，不到8.0，这是主要原因吗？

如需调PH，是用NaOH吗？

电泳分离四种核苷酸时,通常将缓冲液调到什么pH?此时它们是向哪极移动...123

阿瑟6102021-08-09

① 电泳分离4种核苷酸时应取pH3.5 的缓冲液，在该pH时，这4种单核苷酸之间所带负电荷差异较大，它们都向正极移动，但移动的速度不同，依次为：UMP>GMP>AMP>CMP；
　　② 应取pH8.0，这样可使核苷酸带较多负电荷，利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷，但pH过高对分离不利。
　　③ 当不考虑树脂的非极性吸附时，根据核苷酸负电荷的多少来决定洗脱速度，则洗脱顺序为CMP>AMP> GMP > UMP，但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附，嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为：CMP> AMP > UMP >GMP。

蛋白Mops电泳缓冲液是怎么回事儿– 960问答123

浮1502017-10-02

MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、氯霉素溶液等产品由上海哈灵生物有限公司提供

【求助/交流】蛋白电泳缓冲液分子生物综合其他论坛...123

coolaber2021-07-20

北京COOLABER隆重推出PBS、TAE、TBE、蛋白电泳缓冲液独立包装预混干粉试剂，配缓冲液像冲咖啡一样简单，您只需要准备容器和水，就一切OK啦！什么计算配比，什么称重，什么调PH值，都去他的！联系电话：010-8244451715132878855400-878-6800QQ:1002073414李云云

电泳时,缓冲液为弱酸性或中性,DNA为什么会带负电?123

猴琢型182017-10-02

DNA分子在高于其等电点的pH溶液中中带负电,因为所说的等电点是指蛋白质或两性电解质所带净电荷为零时溶液的pH,此时蛋白质或两性电解质在电场中的迁移率为零.当外界溶液的pH大于两性离子的等电点值,两性离子释放质子带负电；当外界溶液的pH小于两性离子的等电点值,两性离子质子化带正电.

电泳缓冲液TAE与TBE,哪个更好? – BioEngX123

coolaber2021-08-03

【求助】跑SDSPAGE的电泳缓冲液需要什么水来配制经验共享 ...123

小聪用的03832017-10-02

跑SDS-PAGE的电泳缓冲液需要什么水来配制，我以前都用一馏水配制，但是有时配制好的电泳缓冲液比较混倒入电泳槽中，都看不清楚琼脂糖封底的下面有没有气泡。最近实验室有人用给细胞配液用的二馏水配制缓冲液，很清澈，跑的电流电压好像也没受什么影响啊，不是说电泳时需要有离子的吗，二馏水里基本是什么都没有了啊。另外，电泳时电流与什么有关，我跑电泳时同样的电压但是电流都不相同，而且现在电流没有以前大了电泳时间也延长很多

【求助】电泳缓冲液多长时间更换一次啊 PCR技术讨论版论坛123

老丽丽2021-08-10

如题啊如题，多久时间换一次好些呢？？:O):O):O):O)

关于电泳缓冲液,奇怪又好笑的问题蛋白质和糖学技术讨论版...123

灬丨牛牛丨灬2021-07-31

笔者小白，做WB时发现了一个奇怪的问题：

电泳缓冲液购买Solarbio的成品（5X），后稀释成1X工作液。

电泳条件：80V120V（注：内外槽每次用的都是新配液体，未使用回收后液体，且泳槽无漏液现象）

电流仪器：Bio-Rad

奇怪的事情在于：当我设定恒定电压为80V跑的时候，查看电流显示为35mA，且电流数值随着时间变化不断降低，半小时后约降至23mA。

请教大神们，之前有没有遇到这样的问题？这倒底是什么原因造成的，应该如何改善？

【求助】为什么我的SDSPAGE电泳时电泳缓冲液变浑浊呢?123