A 1:250 dilution of SPC-1098 was sufficient for detection of WNK1 (pThr60) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Biological Description
Alternative Names
Erythrocyte 65 kDa protein Antibody, HSAN2 Antibody, HSN2 Antibody, hWNK1 Antibody, KDP Antibody, KIAA0344 Antibody, Kinase deficient protein Antibody, MGC163339 Antibody, MGC163341 Antibody, p65 Antibody, PRKWNK1 Antibody, Prostate derived sterile 20 like kinase Antibody, Protein kinase lysine deficient 1 Antibody, Protein kinase lysine-deficient 1 Antibody, Protein kinase with no lysine 1 Antibody, PSK Antibody, Serine/threonine protein kinase WNK1 Antibody, Serine/threonine-protein kinase WNK1 Antibody, With no K Antibody, WNK lysine deficient protein kinase 1 Antibody, WNK1 Antibody, WNK1_HUMAN Antibody
Cellular Localization
Cytoplasm
Accession Number
NP_061852
Gene ID
65125
Swiss Prot
Q9H4A3
Scientific Background
WINK1 is a serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signalling, survival, and proliferation.WNK1 activates SCNN1A, SCNN1B, SCNN1D and SGK1. Exact mechanism of oncogenesis is unclear.
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Biotin
Properties:
Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C10H16N2O3S
Applications: Western blot, immunohistochemistry, and ELISA
① 电泳分离4种核苷酸时应取pH3.5 的缓冲液，在该pH时，这4种单核苷酸之间所带负电荷差异较大，它们都向正极移动，但移动的速度不同，依次为：UMP>GMP>AMP>CMP； ② 应取pH8.0，这样可使核苷酸带较多负电荷，利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷，但pH过高对分离不利。 ③ 当不考虑树脂的非极性吸附时，根据核苷酸负电荷的多少来决定洗脱速度，则洗脱顺序为CMP>AMP> GMP > UMP，但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附，嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为：CMP> AMP > UMP >GMP。
