 
                                            ELISA Kit DIY Materials for Vascular Endothelial Growth Factor D (VEGFD)
FIGF; VEGF-D; C-Fos Induced Growth Factor
- Product No.KSA146Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
 
- Reagent ContentsCapture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.6-1000pg/mL
- Applicable Sensitivity6.4pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM96T*596T*1096T*2096T*5096T*100
- FOBUS$ 896 For more details, please contact local distributors!US$ 1494 For more details, please contact local distributors!US$ 2689 For more details, please contact local distributors!US$ 5229 For more details, please contact local distributors!US$ 8964 For more details, please contact local distributors!
SPECIFITY of the ELISA Kit DIY Materials for Vascular Endothelial Growth Factor D (VEGFD)
The Abs in the kit have high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor D (VEGFD). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor D (VEGFD) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Vascular Endothelial Growth Factor D (VEGFD)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.2. Aspirate and wash 1 time.3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Vascular Endothelial Growth Factor D (VEGFD)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Vascular Endothelial Growth Factor D (VEGFD)
GIVEAWAYS
ELISA / CLIA Experiment Service
INCREMENT SERVICES
CLIA Kit Customized ServiceELISA Kit Customized ServiceAssay Kit DIY Support Pack 1Assay Kit DIY Support Pack 2Standard DiluentCoating BufferDiluent Buffer for AntibodyAssay Kit DIY Support Pack 3Assay Kit DIY Support Pack 4Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) | 
| RPA146Hu01 | Recombinant Vascular Endothelial Growth Factor D (VEGFD) | Positive Control; Immunogen; SDS-PAGE; WB. | 
| EPA146Hu61 | Eukaryotic Vascular Endothelial Growth Factor D (VEGFD) | Positive Control; Immunogen; SDS-PAGE; WB. | 
| APA146Hu01 | Active Vascular Endothelial Growth Factor D (VEGFD) | Cell culture; Activity Assays. | 
| PAA146Hu01 | Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD) | WB; IHC; ICC; IP. | 
| LAA146Hu71 | Biotin-Linked Polyclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD) | WB; IHC; ICC. | 
| MAA146Hu22 | Monoclonal Antibody to Vascular Endothelial Growth Factor D (VEGFD) | WB; IHC; ICC; IP. | 
| SEA146Hu | ELISA Kit for Vascular Endothelial Growth Factor D (VEGFD) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| KSA146Hu01 | ELISA Kit DIY Materials for Vascular Endothelial Growth Factor D (VEGFD) | Main materials for "Do It (ELISA Kit) Yourself". | 
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                                                    百度教育团队【海纳百川团】为您解答。
感谢您的采纳 O(∩_∩)O 。如有疑问,欢迎追问。
2.尿素:有膜蛋白的损失
3.RIPA:用于磷酸化蛋白的温和裂解,不破坏磷酸基团
甲液:0.05mol/L Na2HPO4溶液 :称取磷酸氢二钠9.465g 7.099g,加蒸 馏水至1000ml ;
乙液:0.05mol/L KH2P04溶液: 称取磷酸二氢钾, 9.07g 6.803g,加蒸馏水至1000m1.
将甲乙液分装在棕色瓶内,于4℃冰箱中保存,用时甲、乙两液各按不同比例混合,即可得所需pH的缓冲液,见下表:
pH 甲液ml 乙液mI
5.29 2.5 97.5
5.59 5.0 95.0
5.91 10.0 90.0
6.24 20.0 80.0
6.47 30.0 70.0
6.64 40.0 60.0
6.81 50.0 50.0
6.98 60.0 40.0
7.17 70.0 30.0
7.38 80.0 20.0
7.73 90.0 10.0
8.04 95.0 5.0
你要配制pH=6.8的PBS,可用甲、乙液各50ml混合即可.
根据组成不同,可分为两种,弱酸及其对应的强碱弱酸盐,弱碱及其对应的强酸弱碱盐。
因为HF可以和NaOH反应生成NaF和水,当NaOH反应完之后,NaF就可以与HF组成缓冲溶液,所以说可以直接使用NaOH和HF来配制缓冲溶液。
这也是一般配制缓冲溶液的方法,也就是用强碱和弱酸(或者强酸和弱碱)来配制缓冲溶液。
因为常用的缓冲溶液由弱酸及其共轭酸盐组合而成,当加入酸时,弱酸盐部分转化为弱酸;当加入碱时,弱酸部分转化为弱酸盐。而弱酸在水中不完全电离,弱酸盐在水中可以水解,所以ph变化较小,保持了溶液的PH基本不变。

 
                                     
                    

 
         
         
         
         
                     
                     
                     
                     
             
            