Highlights
- Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
- Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
- Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
Description
| Elution Volume | ≥50 µl |
|---|---|
| Equipment | Microcentrifuge, Vortex |
| Purity | High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 |
| Sample Source | Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized. |
| Size Range | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered |
| Workflow | Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples. |
| Yield | Up to 25 µg total DNA is eluted into ≥50 µl (30 µl minimum) DNA Elution Buffer or water. Human whole blood yields 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg. |
Q1: What is the difference between Quick-DNA and Quick-DNA Plus kits?
The Quick-DNA is optimized for cells, soft tissues, and homogenized/digested samples using a single lysis/binding buffer. The Quick-DNA Plus kits contain an optimized Proteinase K for processing a wider variety of sample inputs, such as cells, blood, tissues, etc. The upgraded Quick-DNA Plus typically recovers more DNA.
Q2: I’m seeing some yield inconsistencies with my blood samples, what’s happening?
White blood cells, which are the major source of genomic DNA in blood, easily and quickly settles. Mix the blood sample well prior to taking an aliquot for purification.
Q3: Can the Quick-DNA kit be used with bacterial samples?
E.coli cells are easy-to-lyse and can be processed directly. For other microbes, additional pretreatment (e.g. enzymatic digestion or mechanical lysis) can be implemented and then processed with the Quick-DNA Kit. Alternatively, for an all-inclusive kit to process all microbes, use any of Zymo Research’s Environmental Kits (e.g. Quick-DNA Fungal/Bacterial, Quick-DNA Fecal/Soil, ZymoBIOMICS DNA, etc.) for DNA isolation.
Q4: Can I use the Quick-DNA kit to clean-up previously isolated DNA?
No, the kit is designed for direct use with biological samples. For clean-up of isolated DNA, please use the Genomic DNA Clean & Concentrator or the DNA Clean & Concentrator kits.
Q5: Can Quick-DNA process crude lysates?
Yes, add 4 volumes of Genomic Lysis Buffer to 1 volume of crude lysate, homogenized, or digested sample (see Cell Suspensions and Proteinase K Digested Samples) and proceed with the remainder of the protocol.
Q6: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?
Beta-mercaptoethanol is a reducing agent that helps break down proteins and improves DNA recovery and purity. Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM) or omitted.
Q7: Is it possible to add an RNase A treatment to the protocol?
The Quick-DNA kits recover RNA-free genomic DNA. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through. No RNase A treatment is required when processing samples within kit specifications.
Q8: What are the expected yields for each sample type?
Keep in mind that there is sample to sample variability.
| Sample Type | Input Amount | Expected Yield |
|---|---|---|
| Eukaryotic Cells | 1x106 Cells | 5-6 µg |
| Skeletal, Heart, Lung, Brain Tissue | 1 mg | 1-3 µg |
| Liver and Kidney Tissue | 1 mg | 3-5 µg |
| Human Whole Blood | 100 µl | 5-7 µg |
“It was easy to work with, protocol easy to follow”
-Tinatin T.
“This kit did a good job of prepping clean genomic DNA.”
-Tara N. (United States Agricultural Research Service)
"This product was amazing! I"ve used the same type of kit (quick DNA extract) from Sigma and this was far more superior. I used the same amount of postnatal tissue as I would have for the Sigma kit, however the yield I obtained from Zymo was quite astounding considering the time of tissue digestion. Secondly, the gDNA was much "cleaner" upon measurement with our NanoDrop! "
-Stephen C. (Johns Hopkins University School of Medicine)
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| C1011-50 | Zymo-Spin IIC Columns | 50 Pack | $55.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| D3004-5-50 | DNA Pre-Wash Buffer | 50 ml | $26.00 | |
| D3004-5-15 | DNA Pre-Wash Buffer | 15 ml | $10.00 | |
| D3004-1-50 | Genomic Lysis Buffer | 50 ml | $34.00 | |
| D3004-1-100 | Genomic Lysis Buffer | 100 ml | $60.00 | |
| D3004-2-50 | g-DNA Wash Buffer | 50 ml | $18.00 | |
| D3004-2-100 | g-DNA Wash Buffer | 100 ml | $30.00 | |
| D3004-4-10 | DNA Elution Buffer | 10 ml | $14.00 |
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在营养学的范畴里，组氨酸被认为是一种人类必需的氨基酸，主要是儿童。在发育多年之后，人类开始可以自己合成它，在这时便成为非必需氨基酸了。在慢性尿毒症患者的膳食中添加少量的组氨酸，氨基酸结合进入血红蛋白的速度增加，肾原性贫血减轻，所以组氨酸也是尿毒症患者的必需氨酸。 在组氨酸脱羧酶的作用下，组氨酸脱羧形成组胺。组胺具有很强的血管舒张作用，并与多种变态反应及发炎有关。此外，组胺会刺激胃蛋白酶与胃酸。
只有9个氨基酸，分子量1KD左右，想免疫小鼠制备单抗，有没有前辈做过这方面的东西？想了一些方法，但价值不大，请前辈们指教，不甚感激。
没有这8种氨基酸就会发生由于缺乏营养所引起的疾病;氨基酸在医药上也有很大的用途，现在手术中输液都加有各种氨基酸，使患者的抵抗力增强，同时也增加营养。随着人民生活水平的提高，人们对摄入的营养物质的要求越来越高，尤其是幼儿、青少年的健康成长，疾病患者的康复，都迫切需要高质量的营养物质，所以有效开发氨基酸食品是很有必要的。
我在看基因方面的文献。基因位点C802T的氨基酸改变H268Y是什么意思啊。求大神指教
