MCAD (MCADherin or cadherin-15) is a 124 kD type I transmembrane glycoprotein of the cadherin superfamily of calcium-dependent homotypic adhesion molecules. 1-4 Like other classical cadherins, the 814 amino acid (aa) human MCAD contains a signal sequence (21 aa), a propeptide (29 aa), an extracellular domain with five cadherin domain repeats (ECD, 556 aa), a transmembrane segment (20 aa) and a cytoplasmic domain (188 aa). 1 A splice variant that diverges within the third cadherin repeat has been sequenced. The cadherin repeats are responsible for cell-cell adhesion by homophilic binding on opposing cells. 1-4 Intracellularly, MCAD binds β-catenin or plakoglobin (γ-catenin), which in turn bind α-catenin. 4 MCAD also binds p120 catenin. 5 Connection of cadherin/catenin complexes to the actin cytoskeleton is in question, but is possible through a linker. 6 Connection to microtubules has been shown. 7 MCAD is present during early stages of skeletal muscle development and is thought to align myoblasts for fusion. 8 It is also present in muscle satellite cells and participates in muscle regeneration. 9 MCAD is also expressed in the granule cell layer of the cerebellar glomerulus. 10 Deletion of mouse MCAD has little effect in vivo, most likely due to compensation by N-cadherin. 11 However, MCAD upregulation and adhesion between myoblasts during induction of differentiation in vitro is required for their fusion. 8, 12, 13 MCAD activity is later downregulated by sequestering to caveoli, p120 catenin/RhoA-induced ubiquitination and/or cleavage by calpain-3. This terminates fusion and allows sarcomere formation. 5, 12, 13 Human MCAD ECD shows 88% aa identity with mouse, rat or cow, and 85% aa identity with dog MCAD ECD. MCAD is an outlier among classical cadherins, with 40% aa identity or less in the ECD. 4||Source: Human MCAD (Met 1-Ala 606), IEGRMD, Human IgG1 (Pro 100-Lys 330); A DNA sequence encoding the extracellular domain of human M-cadherin (Met 1-Ala 606; Accession # P55291) (Shibata, T. et al., 1997, J. Biol. Chem. 272:5236-40) was fused to the Fc region of human IgG1 via a polypeptide linker. The chimeric protein was expressed in a mouse myeloma cell line, NS0.||Molecular Mass: The recombinant human MCAD/Fc is a disulfide-linked homodimeric protein. Based on N-terminal sequencing, the recombinant pro-protein starts at Val 22 and has a calculated molecular mass of 90.5 kD. As a result of glycosylation, the recombinant human MCAD/Fc monomer migrates as an approximately 122 kD protein in SDS-PAGE under reducing conditions.||Endotoxin Level: <1.0 EU per 1 μg of the protein as determined by the LAL method.||Activity: Measured by the ability of the immobilized protein to support the adhesion of C2C12 mouse myoblast cell line stimulated for 36 hours with 10% equine serum. When 3 x 10e4 cells/well are added to rhMCAD/Fc coated plates (6ug/ml, 100 μL/well), approximately 35 – 60% will adhere after 1 hour at 37° C.||Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.||Storage: Lyophilized samples are stable for up to twelve months at -20°C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2°-8° C for one month or at -20°C in a manual defrost freezer for three months without a significant loss of activity. Avoid repeated freeze-thaw cycles.Source: Mouse myeloma cell line, NS0|Purity: ≥ 90% as determined by SDS-PAGE and visualized by silver stain.|Concentration: As reported|Form: Supplied as a lyophilized powder in 20mM MES, 0.2M NaCl, 2mM CaCl2, pH 6.5.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

USBIo全称United States BIOLOGical，位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商，目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂，以质量**闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商，产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。产品精确度高，质量稳定，产品数量巨大，提供70,000多种抗体、抗原和生化试剂等。

Usbio的生物化学品，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。USBio产品从经济实惠的研究数量到更大的批量可用于工艺开发和生产。我们的客户包括位于美国和国外的大多数*的制药和生物技术公司。

USbiological公司致力于以价值，诚信和真正的个人购买体验降低研究成本。USbiological的生物化学，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。产品可以从负担得起的研究数量到大批量用于工艺开发和生产。

USBio（UnitedStatesBiological，或简称USBiological）位于麻省斯万普斯科特(Swampscott,MA)。作为全美最大的抗原抗体和生化试剂供应商，USBiological目前向全球六十余个国家的科研工作者提供约150,000种抗体、抗原和生化试剂，以质量卓越闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国USBiological的产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。USBiological一直恪守“为您的研究减少成本”原则，提供高纯度、高品质的产品，并在大多数生化试剂、生物与培养基产品线上保持最高的性价比。USBiological的产品被广泛用于各种科学研究领域，包括基因组研究、生物技术、药物研发和疾病诊断等。USBiological的产品从小型科研级到生物制品大规模级规格均有提供。众多全球顶ji的生物制药和生物技术公司都在使用USBiological的高品质产品。

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。

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