BP-10 Spin Column DNA Cleanup Minipreps Kit
Components | CMIP-50, 50 Preps | CMIP-100, 100 Preps |
Cleanup Solution Wash Solution Elution Buffer BP-10 Column 2.0 ml Collection Tube Protocol | 20 ml 12 ml 5 ml 50 50 1 | 40 ml 24 ml 10 ml 100 100 1 |
1. Before use, add 48 ml of 100% of ethanol to 12 ml
2. Elution Buffer is 2.0 mM Tris-HCl pH 8.0~8.5.Although TE buffer pH 8.0 or water can be used, yield is generally 20% lower.
Storage: The kit is stable for 12 months at room temperature. For longer storage, keep all contents of the kit cold.
Principle:
This DNA Cleanup kit provides a simple, efficient method for purification of DNA fragments from variable enzymatic reactions such as cDNA synthesis, ligation, restriction digestions, tailing, PCR, alkaline phosphatase, nick translation, due terminators products from PCR* reaction mixture. It is also an ideal tool to desalt the solution of DNA as well as to remove residual organic solvents or unincorporated nucleotides or primers (<40-mer) from reaction mixture. The kit utilizes a technology which adsorbs selectively up to 10ug DNA fragments in the column in the presence of specialized binding buffers. Nucleotides, enzymes, mineral oil and other impurities do not bind to the columns in the plate. DNA fragments can be eluted readily with elution buffer.
Application:
- DNA Cleanup from the enzymatic reactions
- Removal of nucleotides and primers ( <40-mer)
Features:
- Rapid and economical. Entire procedure takes 40 minutes.
- High yields (60-80%). It is suitable to recover 100 bp-40 kb DNA fragments.
- Efficient removal of contaminants. Purified DNA can be used in any downstream applications such as sequencing, labeling, restriction enzymatic digestions, ligations or transformations.
- No phenol / chloroform extraction or ethanol precipitation
Procedure for Purification of DNA Products:
1. Transfer DNA mixture to a 1.5 ml microfuge tube and add 3 volumes of Cleanup Solution .
2. Place a column into a 2.0 ml collection tube. Transfer the above mixture solution to the column, and let the column stand at room temperature for 2 minutes. Spin at 5000 rpm for 1 minute.
3. Discard flow-through. Add 500 ul Wash Solution to the column and spin at 8,000 rpm for 1 minute. Discard flow-through and place column back to the same collection tube.
4. Add 500 ul Wash Solution to the column, spin at 8,000 rpm for 1 minute. Discard flow-through and spin once more to remove residue of Wash Solution.
5. Transfer column to a clean 1.5 ml microtube. Add 30-50ul Elution Buffer or water onto the center part of the column, incubate at 50 oC for 2 minutes. Spin down at 10,000 rpm for 1 minute. Purified DNA including PCR product is ready for use or kept at -20oC.
Note:
1. Incubation at 37-50oC can improve recovery yield.
2. If PCR reaction mixture contain seriously non-specific amplified DNA fragments, use of DNA Gel Extraction Kit is recommended.
3. This kit can not remove the template and primers with chain length longer than 50-mer.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
1.CA15-3属于多形上皮黏蛋白。CA15-3测定广泛用于乳腺癌的辅助诊断。60%～80％进展期乳腺癌患者的CA15-3浓度大30kU/L。CA15-3有助于判断肿瘤的进展和疗效，测定值大于1000kU/L者预后不良。在乳腺癌初期其灵敏度较低，在Ⅲ期时约为12%，因此CA15-3测定不能用作乳腺癌的筛选手段．当怀疑乳腺癌而CA15-3又正常时，可以结合CEA值进行考虑。应注意到治疗后由于肿瘤细胞死亡，CA15-3起初会升高，然后才逐渐下降。持续不降或上升者则提示病情恶化。
2．其他恶性肿瘤，如肺、结肠、胰腺、肝脏、卵巢癌、子宫颈和子宫内膜的恶性肿瘤也都可见到CA15-3不同程度的阳性率（低于10%）。
3．肝硬化、肝炎、结节病、结核病、自身免疫性疾病（SLE)以及卵巢、乳腺的良性病变中，CA15-3值略高于正常范围，但阳性率一般低于10%，妊娠中后期，有近一半孕妇血清CA15-3升高。
核酸是天然高分子化合物，，糖类中的多糖（淀粉、纤维素、糖原等）也是天然高分子化合物
同属于生命的六大营养物质（水，无机盐，糖类，脂质，核酸，蛋白质）
还有如果用气相做糖的鉴别的话，需要样品量大不大？怎么衍生化？
不好意思，我是学药分的，对植化比较外行，请详细些，谢了先！
