DescriptionViPrimePLUS Taq qPCR Green Master Mix I is next generation master mix designed for fast and easy realtime PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains all the reagents, including Taq DNA Polymerases, SYBR® Green dye, highest quality dNTPs, and buffer components at optimal concentrations. Taq DNA Polymerases in the master mix provide excellent results in reaction efficiency, correlation coefficient and slope. SYBR® Green dye emits fluorescence when bound to double-stranded DNA. Detection of PCR product is monitored by the increase in fluorescence, leading to high sensitivity, wide dynamic range and high reproducibility for quantification.ViPrimePLUS Taq qPCR Green Master Mix I can be used to amplify any DNA template including genomic, cDNA and viral sequences. The formulation of qPCR green master mix can detect low copy number targets very specifically with high efficiency. The qPCR green master mix provides convenient and robust set up for quantitative real-time analysis of DNA samples.ViPrimePLUS Taq qPCR Green Master Mix I has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS Taq qPCR Green Master Mix I in standard cycling conditions gives the industry leading performance in fast cycling conditions.
ApplicationsAll kinds of sample material suited for qPCR amplification can be used.
Features
- Ready-to-use real-time PCR reaction set up
- Rapid extension rate for early Ct values
- Good buffer system for excellent amplification efficiency
- Includes SYBR® Green dye for intercalator-based qPCR
- Increased limit of detection
- Compatible with most of the real-time PCR platforms
Component1.6ml aliquots of master mix
StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS Taq qPCR Green Master Mix I has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Product Description | Compatible Hardware |
| QLMM12ViPrimePLUS Taq qPCR Green Master Mix I (SYBR® Green Dye) | Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™ |
| QLMM12-LRViPrimePLUS Taq qPCR Green Master Mix I with Low ROX (SYBR® Green Dye) | Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| QLMM12-RViPrimePLUS Taq qPCR Green Master Mix I with ROX (SYBR® Green Dye) | Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM12 | ViPrimePLUS Taq qPCR Green MasterMix I (SYBR® Green Dye) | 150 reactions |
| QLMM12-LR | ViPrimePLUS Taq qPCR Green MasterMix I with Low ROX (SYBR® Green Dye) | 150 reactions |
| QLMM12-R | ViPrimePLUS Taq qPCR Green MasterMix I with ROX (SYBR® Green Dye) | 150 reactions |
DownloadManual
ViPrimePLUS Taq qPCR Green MasterMix I (SYBR® Green Dye) ViPrimePLUS Taq qPCR Green MasterMix I with Low ROX (SYBR® Green Dye) ViPrimePLUS Taq qPCR Green MasterMix I with ROX (SYBR® Green Dye)
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1.CA15-3属于多形上皮黏蛋白。CA15-3测定广泛用于乳腺癌的辅助诊断。60%～80％进展期乳腺癌患者的CA15-3浓度大30kU/L。CA15-3有助于判断肿瘤的进展和疗效，测定值大于1000kU/L者预后不良。在乳腺癌初期其灵敏度较低，在Ⅲ期时约为12%，因此CA15-3测定不能用作乳腺癌的筛选手段．当怀疑乳腺癌而CA15-3又正常时，可以结合CEA值进行考虑。应注意到治疗后由于肿瘤细胞死亡，CA15-3起初会升高，然后才逐渐下降。持续不降或上升者则提示病情恶化。
2．其他恶性肿瘤，如肺、结肠、胰腺、肝脏、卵巢癌、子宫颈和子宫内膜的恶性肿瘤也都可见到CA15-3不同程度的阳性率（低于10%）。
3．肝硬化、肝炎、结节病、结核病、自身免疫性疾病（SLE)以及卵巢、乳腺的良性病变中，CA15-3值略高于正常范围，但阳性率一般低于10%，妊娠中后期，有近一半孕妇血清CA15-3升高。
核酸是天然高分子化合物，，糖类中的多糖（淀粉、纤维素、糖原等）也是天然高分子化合物
同属于生命的六大营养物质（水，无机盐，糖类，脂质，核酸，蛋白质）
还有如果用气相做糖的鉴别的话，需要样品量大不大？怎么衍生化？
不好意思，我是学药分的，对植化比较外行，请详细些，谢了先！
