Details
Nru I Source
Nocardia rubra
Reagents Supplied
CMX Buffer 3
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of unmethylated lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
No
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
1 mM dithiothreitol
100 mM NaCl
100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 0%
Buffer 2: 25%
Buffer 3: 100%
Buffer 4: 25%
Buffer 5: 50%
Storage Buffer
50 mM Tris‑HCl (pH 7.5 at 4°C)
100 mM KCl
1.0 mM EDTA
10 mM β-mercaptoethanol
500 µg/ml bovine serum albumin
0.1 mM phenylmethyl sulfonyl fluoride
50% (v/v) glycerol
Assay Conditions
50 mM Tris‑HCl (pH 7.5 at 37°C)
10 mM MgCl2
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg unmethylated lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°C
Shipped on dry ice
Nru I Downloads
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
Categories
Restriction Endonucleases
ebiomall.com
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希望同路人多多交流啊!
这些染料都非常成熟,光毒和淬灭都很低,当然要考虑到你采集图像时的显微镜参数。
比如calcein,常用的示踪,绿光(虽然这些颜色只是根据光谱加上去的伪彩),但要考虑你的实验过程中结合细胞结构,是否会伴随calcein的泄漏,就是荧光降低。
dri,还可以看膜啊
cfda也不错
bcef虽是ph指示,但你试验时不仅可观察细胞,还可看细胞ph变化,也行。
当然你或许还要结合其他方法,如细胞免疫化学等手段去双染或多染,都需要综合考虑染料之间特性。单独染一个染料,有点浪费,不如多染,数据和图像也好看些。现在流行细胞成像。

