Details
RsrIISource
Rhodopseudomonassphaeroides
ReagentsSupplied
CMXBuffer4
UnitDefinition
Oneunitistheamountofenzymerequiredtodigest1µgoflamBDaDNAwith≥95%efficiencyin1hourinatotalreactionvolumeof50μl
StarActivity
No
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer4:
20mMTris-Acetate(pH7.5at37ºC)
50mMPotassiumAcetate
10mMMagnesiumAcetate
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:50%
Buffer2: 75%
Buffer3:25%
Buffer4:100%
Buffer5:50%
StorageBuffer
10mMTris‑HCl(pH7.5at4ºC)
0.1mMEDTA
50mMKCl
1mMdithiothreitol
200µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
20mMTris‑Acetate(pH7.5at37ºC)
50mMPotassiumAcetate
10mMMagnesiumAcetate
1.0mMdithiothreitol
100µg/mlbovineserumalbumin
1µglambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
Notes
- LambdaDNAcontains3slow-cleavagesitesthatdonotcompletelydigestafter1hourincubation.
- Completedigestionofthesesitesisachievedby4UnitsofRsrIIwith1μgoflambdaDNAincubatedat37°Cfor16hours.
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases
ebiomall.com
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希望同路人多多交流啊!
这些染料都非常成熟,光毒和淬灭都很低,当然要考虑到你采集图像时的显微镜参数。
比如calcein,常用的示踪,绿光(虽然这些颜色只是根据光谱加上去的伪彩),但要考虑你的实验过程中结合细胞结构,是否会伴随calcein的泄漏,就是荧光降低。
dri,还可以看膜啊
cfda也不错
bcef虽是ph指示,但你试验时不仅可观察细胞,还可看细胞ph变化,也行。
当然你或许还要结合其他方法,如细胞免疫化学等手段去双染或多染,都需要综合考虑染料之间特性。单独染一个染料,有点浪费,不如多染,数据和图像也好看些。现在流行细胞成像。

