Product Specifications:
Item# 1041: Recombinant HIV-1 IIIB Glycoprotein gp120 (CHO)
Concentration: See vial
Diluent: PBS
Purity: >95%
Stabilizer: None
Preservative: None
Storage: -75°C
Physical State: Frozen
Liquid Stability: At least 6 months at -75°C.
Applications: In-Vitro Diagnostics, CD4 Binding, T-Cell Activation.
Description: Full length Recombinant HIV-1 IIIB gp120 glycoprotein produced in the CHO Expression System.
Purification: This protein is purified by immunoaffinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of solid phase protein under standard ELISA conditions.
Item#: 1041-P Peroxidase-Conjugated rgp120 HIV-1 IIIB
Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies.
Safety Study of rgp120/HIV-1IIIB Vaccine
Recent evidence suggests that gp120 is the HIV-1 protein with the greatest potential as a vaccine against HIV-1 infection. The gp120 envelope protein may be produced by recombinant DNA technology, and studies have shown that the vaccine is capable of eliciting neutralizing antibody activity in both rodents and nonhuman primate species.
Official Study Title: A Phase I Study of the Safety and Immunogenicity of rgp120/HIV-1IIIB Vaccine in Healthy Adult Subjects (NOTE: Study Extended ONLY for Subjects Who Have Previously Received rgp120/HIV-1IIIB or rgp120/HIV-1MN on VEU 006 or VEU 006 Rollover Study)
Disease: HIV Infections
Treatment: Biological: rgp120/HIV-1IIIB and Biological: rgp120/HIV-1MN
ORIGINAL DESIGN: Twenty-eight subjects will be randomized to receive 100 or 300 mcg rgp120/HIV-1IIIB vaccine (gp120 vaccine) or matching placebo. For each dose level, 10 subjects will receive vaccine and four subjects will receive matching placebo. Injections are given intramuscularly at 0, 4, and 32 weeks. Each subject receiving treatment at the lower dose level must be monitored for unacceptable toxicity for at least 2 weeks following the initial immunization before his or her second dose is administered and before treatment at the higher dose level begins. Subjects are followed for at least 12 months.
AMENDED 11/17/93: Selected subjects from VEU 006 or VEU 006 Rollover study will receive two injections of MN rgp120 vaccine, administered 28 days apart beginning 10-16 months after their last injection. Eight additional clinic visits will be required. Subjects are followed for at least 6 months.
CHO- expressed HIV-1IIIB rgp120 (2.5 μg) and biotinylated cyclic V2-TH023 peptide (1 μg and 5 μg) were incubated with RPMI8866 cells
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颜料是用着色的物质通常与被着色的物质混合在一起主要以无机化合物为主；染料是一种用来直接或经媒染剂作用而能附着在各种纤维和其他材料上的有色物质有的可以跟被染物质化合，多以有机化合物为主。 颜料不能上色,而染料能上色. 颜料和染料的区别 颜料是一种微细粉末状的有色物质,一般不溶于水、油和溶剂,但能均匀的分散在其中。颜料是色漆的次要成膜物质,在木材装饰过程中调制底漆、腻子以及木才着色,也经常使用颜料.
我想用共聚焦观察间期染色体，用涂染探针，有一个问题很困扰我，我想涂染完染色后用DAPI复染细胞核，但是目前哈尔滨的共聚焦都没有紫外激发光，不能激发DAPI，我怎么才能实现，用红色涂一条染色体，用绿色涂另一条，用DAPI蓝色染核，同时成像呢，所说的双光子显微镜可以么？我实在很迷惑，求求版主别再删我的帖子，尽管我现在只是一个索取者，还不能给大家提供有用的信息，但是相信有一天会为大家做贡献的，谢谢了
荧光增白剂是一种荧光染料，也是一种复杂的有机化合物，其特性就是能产生蓝色荧光，可使肉眼看到的物质很白。
测试面膜是否含有荧光增白剂，打开面膜的包装，然后放到“ZF-C型三用紫外分析仪”下，使用紫外光照射面膜，看其是否会发出亮蓝色光，如果有，说明面膜上含有荧光剂。 如果没有紫外分析仪，可以用紫外手电筒，紫外验钞机/笔等。
含荧光剂的面膜在紫外光照射下，显现出非常大面积的亮蓝色。稍微接触面膜液体，就会粘附上荧光剂成分，拿了面膜的手，也有荧光。用清水冲洗手3遍后， 手上的荧光剂在紫外光照射下依然发出强烈的亮蓝色光，几乎没有洗掉。改用洗手液清洗3遍，照射后手上也发出蓝光，只是比清水的效果略好一些。用洗面奶清洗，与洗手液的效果差不多，都无法彻底清洗掉残留在手上的荧光...
荧光剂对人体的危害：
荧光剂不像一般化学成分那样容易被分解，而是在人体内蓄积，产生许多有害的作用，大大削减人体免疫力；荧光剂与伤口外的蛋白质结合，还会阻碍伤口的愈合；荧光剂能使人体细胞出现变异性倾向，其毒性累积在肝脏或其他重要**，会成为潜在的致癌因素。造成血液系统受损：化学物质容易污染人体血液，虽然血液具有一定的自净能力，微量的有害物质进入其中，会被稀释、分解、吸附和排出，但长期、大量的有毒物质倾注而入，必致其发生质的变化；进入血液循环，会破坏红细胞的细胞膜，引起溶血现象。
