| Overview |
 Printer Friendly Version
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| Ex/Em (nm) | 498/520 |
| MW | N/A |
| CAS # | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category |
Cell Biology Labeling Cells |
| Related |
Fluorescence Imaging Biochemical Assays |
| Spectrum | Advanced Spectrum Viewer |
1. Prepare 1X iFluor™ 488-Phalloidin working solution:
Add 10 µL of iFluor™ 488-Phalloidin (Component A) to 10 mL of Labeling Buffer (Component B).
Note 1: The unused 1X iFluor™ 488-Phalloidin stock solution should be aliquoted and stored at -20 ºC. Protect from light.
Note 2: Different cell types might be stained differently. The concentration of iFluor™ 488-Phalloidin working solution should be prepared accordingly.
2. Stain the cells:
2.1 Perform formaldehyde fixation. Incubate the cells with 3.0–4.0 % formaldehyde in PBS at room temperature for 10–30 minutes.
Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.
2.2 Rinse the fixed cells 2–3 times in PBS.
2.3 Optional: Add 0.1% Triton X-100 in PBS into fixed cells (from Step 2.2) for 3 to 5 minutes to increase permeability. Rinse the cells 2–3 times in PBS.
2.4 Add 100 μL/well (96-well plate) of iFluor™ 488-Phalloidin working solution (from Step 1) into the fixed cells (from Step 2.2 or 2.3), and stain the cells at room temperature for 15 to 60 minutes.
2.5 Rinse cells gently with PBS 2 to 3 times to remove excess dye before plate sealing and imaging by using FITC channel.
| References & Citations |
 Citation Explorer
|
Porous Li-containing biphasic calcium phosphate scaffolds fabricated by three-dimensional plotting for bone repair
Authors: Xiaoheng Guo, Huichang Gao, Xiao Liu, Jingjing Diao, Xuetao Shi, Naru Zhao, Yingjun Wang
Journal: RSC Advances (2017): 34508--34516
Preparation, characterization and in vitro cell performance of anti-washout calcium phosphate cement modified by sodium polyacrylate
Authors: Xingmei Li, Fupo He, Jiandong Ye
Journal: RSC Advances (2017): 32842--32849
miR-29b-Loaded Gold Nanoparticles Targeting to the Endoplasmic Reticulum for Synergistic Promotion of Osteogenic Differentiation
Authors: Ting Pan, Wenjing Song, Huichang Gao, Tianjie Li, Xiaodong Cao, Shizhen Zhong, Yingjun Wang
Journal: ACS Applied Materials & Interfaces (2016): 19217--19227
Osteogenic and tenogenic induction of hBMSCs by integrated nanofibrous scaffold with chemical and structural mimic to bone-ligament connection
Authors: Zifeng Lin, Xiujuan Zhao, Si Chen, Chang Du
Journal: Journal of Materials Chemistry B (2016)
The stimulation of the differentiation of pheochromocytoma (PC12-L) cells into neuron-like cells by electrically conductive nanofibre mesh
Authors: Huishang Yang, Guanglin Zhu, Yicheng Huang, Xuetao Shi, Yingjun Wang
Journal: Applied Materials Today (2016): 215--222
Distinct mechanical behavior of HEK293 cells in adherent and suspended states
Authors: Seyed Mohammad Ali Haghparast, Takanori Kihara, Jun Miyake
Journal: PeerJ (2015): e1131
The preparation and characterization of polycaprolactone/graphene oxide biocomposite nanofiber scaffolds and their application for directing cell behaviors
Authors: Juqing Song, Huichang Gao, Guanglin Zhu, Xiaodong Cao, Xuetao Shi, Yingjun Wang
Journal: Carbon (2015): 1039--1050
Actin-based biomechanical features of suspended normal and cancer cells
Authors: Seyed Mohammad Ali Haghparast, Takanori Kihara, Yuji Shimizu, Shunsuke Yuba, Jun Miyake
Journal: Journal of bioscience and bioengineering (2013): 380--385
AAT Bioquest AAT Bioquest是一家位于美国的生物公司，前身为ABD Bioquest，总部位于加利福尼亚州。专门从事光学检测技术十多年，一直致力于光谱学检测领域技术的创新和突破。其独特的光学检测技术，综合了化学、生物学和信息学等各个领域的研究，引领了比色、荧光和发光技术新一代光学探针的浪潮。AAT Bioquest在全球拥有强大的经验丰富的专业分销商网络，为从小型研究机构到《财富》500强企业的各类客户提供卓越的产品和定制服务。
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(1)、分子内含有发射荧光的基团，如羰基、氮氮双键、碳氮双键等。
(2)、分子内含有助色基团。助色基团使光谱红移并增大荧光效率，如伯胺基、仲胺基、羟基、醚键、酰胺基等。
(3)、分子内含有刚性平面结构的共轭π键。分子内共轭体系愈大平面性愈强其荧光强度愈高。一些能提高共轭度的因素能提高荧光效率，并使荧光波长向长波方向移动。 就是荧光染料的附着物，主要作用有帮助荧光染料展色、提高荧光染料与下游树酯的相溶性、保护荧光染料的性能。通常载体树脂是强极性树脂，分子中含有胺基、羟基、醚键、酰胺基等强极性基团，一方面有助色作用，增大荧光效率;另一方面与荧光染料有很好的相溶性，有助于染料的均匀分散。
荧光颜料常用的载体树脂有胺基树脂、苯代三聚氰胺一甲醛树脂、聚丙烯酸酯树脂、聚酰胺树酯、聚酯树脂、聚氨酯树脂等。 （1）、热塑性荧光颜料：线型
（2）、热固性荧光颜料： 体型
（3）、可溶解色精荧光颜料
（4）、水乳型荧光颜料 （1）、胺基树酯
（2）、聚酰胺树酯
（3）、聚酯树酯
（4）、丙烯酸乳液 （1）、塑胶类
低温型
中温型
高温型
（2）、涂料类
水性涂料
油性涂料
粉末涂料 （1）、含甲醛
（2）、不含甲醛
希望同路人多多交流啊!
想请问一下，DAPI这个染料到底有没有膜通透性，我通过百度搜索查询关于DAPI染料的，基本上是说它能透过细胞膜对活细胞和死细胞均能染上蓝色；但是也有人说DAPI只可以透过死细胞膜，不能对活细胞进行染色，用以区分活死细胞，到底哪个是对的啊，蒙了！！！！！！
