
Format : | Purified |
Amount : | 100 µg |
Isotype : | Rabbit IgG, kappa |
Purification : | Purified Ab with BSA and Azide at 200ug/ml |
Content : | 200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Gene : | CHGA |
Gene ID : | 1113 |
Uniprot ID : | P10645 |
Alternative Name : | Beta-Granin,CGA,CHGA,Chromogranin A Parathyroid Secretory Protein 1,ER-37,Pancreastatin,Parastatin,Pituitary Secretory Protein I,SP-I,Vasostatin I or II |
Immunogen Information : | Recombinant full-length human CHGA protein |
Does not react with rat.
MW : 68-75kDa; Positive Control : PC12 cells. Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma.;Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Western Blotting (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Secreted, Cytoplasmic vesicle |
Post transnational modification: | Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584). Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725). |
Tissue Specificity: | GE-25 is found in the brain. |
BioGrid: | 107538.12 interactions. |
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请问各位大神,配制琼脂糖胶的电泳浓度是怎么设定,比如百分之1或百分之1.5的浓度是怎么设置,是1.5g的琼脂糖粉加100g的TAE麽?
琼脂糖 从琼脂中提取的、由不同类型吡喃半乳糖聚合而成的多糖。在生物化学和分子生物学研究中用于凝胶过滤、凝胶电泳和凝胶扩散实验
琼脂糖是线性的多聚物,基本结构是1,3连结的β-D-半乳呋喃糖和1,4连结的3,6-脱水α-L-半乳呋喃糖。琼脂果胶是由许多更小的分子组成的异质混合物。它们的结构相似,但带硫酸根和羧基组分,凝胶能力差。
琼脂糖(Agarose)的特性详细介绍可以登录生物帮看看, http://www.bio1000.com/news/cp/ 生物产品,生物学新技术,生物研发,生物产业,生命科学创新产品。
biowest agarose 指的是西班牙琼脂糖。
agarose指的是琼脂糖。
注意DNA酶污染的仪器可能会降解DNA,造成条带信号弱、模糊甚至缺失的现象。 电泳洗脱法
低熔点琼脂糖凝胶电泳挖块法
冻融回收法
玻璃奶回收法
柱回收法 将电泳槽用ddH2O反复清洗干净,倒入新鲜配制的灭菌电泳缓冲液;
根据点样量制备合适厚度的琼脂糖凝胶板;
切胶时尽可能切掉不含DNA片段的凝胶;
要尽量减少DNA在紫外下的照射时间以减少对DNA的损伤;
熔胶要完全。向左转|向右转
1、α-琼脂糖酶及其生产方法
2、磁性琼脂糖复合微球的制备方法
3、高容量大孔琼脂糖凝胶介质的制备方法
4、一种新琼四、六糖的制造方法
5、一种制造新琼八糖、新琼十糖和新琼十二糖的方法
6、油水两相法制备磁性琼脂糖凝胶微球的方法
中文名称:琼脂糖中文别名:琼脂糖ME英文名称:Agarose英文别名:Agarose, medium EEO, Molecular Biology Grade; agarose, mixture; Agaroseforelectrophoresisofmacromolecules;agarose for molecular biology; agarose 6 B for chromatography; Agarose LE; sepharose(R) 4B for chromatography;CAS:9012-36-6;62610-50-8EINECS:232-731-8分子式:C24H38O19分子量:630.5471安全术语:S24/25:Avoid contact with skin and eyes.;
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