产品描述
RegularagaroseGradeCatalogNO.STA1200
Agarose
Storedryatroomtemperature
Descriptions:Size:Formulation:
100g
WhitePowder
Introduction:
Routineuseagaroseisidealforeverydayanalysisofnucleicacidsbygelelectrophoresisorblotting(NorthernorSouthern)andisalsosuitableforproteinapplicationssuchasOuchterlonyandrADIalimmunodiffusion(RID).
Data:
EEO(electroendosmosis)(-Mr)
0.15
Watercontent
≤10%
Sulfate(SO4)
≤0.15%
Gelstrength(1.0%gel)
≥1000/cm2
Meltingpoint(1.0%gel)
87.5±1.5℃
Gellingpoint(1.0%gel)
35.5±1.5℃
Foreignactivity
DNase,RNase,nonedetected
AnalysisNote:
Sulfatecontent-usedasanindicatorofpurity,sincesulfateisthemajorionicgrouppresent.
Gelstrength-theforcethatmustbeappliedtoageltocauseittofracture.
Gelpoint-thetemperatureatwhichanaqueousagarosesolutionformsagelasitcools.Agarosesolutionsexhibithysteresisintheliquid-to-geltransition-thatis,theirgelpointisnotthesameastheirmeltingtemperature.
Electroendosmosis(EEO)-amovementofliquidthroughthegel.Anionicgroupsinanagarosegelareaffixedtothematrixandcannotmove,butdissociablecountercationscanmigratetowardthecathodeinthematrix,givingrisetoEEO.Sinceelectrophoreticmovementofbiopolymersisusuallytowardtheanode,EEOcandisruptseparationsbecauseofinternalconvection.
PreparationofAgaroseGelsforDNAseparations:
WeighoutthedesiredamountofagaroseandplaceinanErlenmeyerflaskwithameasuredamountofelectrophoresisbuffer,e.g.foran0.8%gel,add0.8gmofagaroseand100mlofTBEBuffer(1X),toa200mlflask.Thelargerflaskinsuresagainsttheagaroseboilingover.Dissolvetheagaroseinaboilingwaterbathorinarevolving-platemicrowaveoven.Allthegrainsofagaroseshouldbedissolvedandthesolutionclear.Coolthesolutionto60°C(70°Cforconcentrations2%orabove)andpourimmediately.Allowthegeltosetforone-halfhourbeforeusing.Makesuretousethesameelectrophoresisbufferinthegelasfortherunningbuffer.
