Properties:
| Related Categories | Analytical/Chromatography, Clear glass vials, Most Popular Vial Products, Precleaned Sampling Vials, Silanized & Precleaned Vials, |
| material | PTFE/silicone septa (Thickness: 0.125") |
| clear glass vial | |
| white polypropylene cap | |
| feature | closure type screw top vial |
| closure type white polypropylene hole cap | |
| packaging | pkg of 72 ea |
| vial O.D. × H | 29 mm × 82 mm |
Description:
General description
Each of the vials is cleaned according to US EPA Protocol B: The cleaning procedure is as follows:
1. Wash containers in hot water using laboratory grade non-phosphate detergent.
2. Rinse three times with copious amounts of tap water to remove detergent.
3. Rinse three times with ASTM Type I organic-free water.
4. Oven-dry containers.
5. Allow containers to cool to room temperature in an enclosed contaminant-free environment.
6. Cap the containers.
After being cleaned these vials are assembled with the following materials: Type 1 borosilicate glass, PTFE/silicone septa, and a polypropylene cap. The vials are available in either clear or amber glass. All of the vials are packaged with an open top cap and a tan/white PTFE/silicone septa.
The 40 mL EPA vials are supplied with a Certificate of Analysis and a package of labels to use to identify the contents of your sample. The package of vials is shrink wrapped after cleaning to make sure that the vials arrive to you as clean as possible.
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如常用于缓冲的Tris、HEPES、MPOS等等,去垢剂如NP40,Tween-20,TritonX100,CHAPS,脱氧胆酸钠,SDS等等,
大家能说说这些试剂的使用区别吗(最好讲讲某些情况下使用一些会优于另一些的例子)
有时候某些裂解液含10%左右甘油,又是为何?
平时我们用习惯了一些配方,可能有时候不太清楚为何有些是可替代的,有些是不可替代的,借此机会想听听大家的见解?
如要膜蛋白保持原来的结构应采用:非离子型去垢剂.
B.阴离子去垢剂 常见的有十二烷基硫酸钠和十二烷基璜酸钠。前者可促进核蛋白的溶解,将核酸释放出来,并对核酸酶有一定抑制作用,常用于核酸的提取。
C.阳离子去垢剂 如洁尔灭、新洁尔灭、CTAB、CPC、ZEPH、克菌定、消毒净(TMPB)、杜灭芬等,消毒灭菌类居多。
D.天然表面活性剂 又称为生物表面活性剂,包括种类较为广泛,如各种树胶(阿拉伯胶、杏胶、桃胶、果胶)、明胶、皂甙、卵磷脂、豆磷脂、琼脂、海藻酸钠、酪蛋白、胆甾醇、胆酸类、多糖类(如环糊精)等。
E.两性表面活性剂 在碱性水溶液中呈阴离子表面活性剂的性质,起泡性好,去污力也强;在酸性溶液中则呈现阳离子表面活性剂特征,其杀菌性很强。向左转|向右转
我要做的两个目的蛋白,分子量一个是140的,另一个是36,内参用的GAPDH。这几天刚刚要做小鼠组织的蛋白,就把新提取的组织蛋白和之前做过的细胞蛋白一起上了,细胞和组织的各上了3个孔。
电泳转膜孵育等条件都是以前做成熟的
之后显影,细胞蛋白的,两个目的和内参都出得挺好;但是小鼠的组织蛋白只有分子量140的那个显出条带了,36的那个显出来的像一片水渍一样,斑片状的黑,内参有时也这样、有时干脆什么都不显。不知道这是什么原因...按说细胞同样的蛋白做出来,WB的各个步骤应该不会有太大问题的。
有点怀疑蛋白提取的不好,我提组织蛋白的的操作是在冰上进行的,裂解液也加有PMSF,用BCA法测得的蛋白浓度大概也有3~5mg/ml。
在这里求助各位高手~望给我指点一下~不胜感激啊!

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