
Properties:
Related Categories | Analytical/Chromatography, Clear glass vials, Most Popular Vial Products, Precleaned Sampling Vials, Silanized & Precleaned Vials, |
material | PTFE/silicone septa (Thickness: 0.125") |
clear glass vial | |
white polypropylene cap | |
feature | closure type screw top vial |
closure type white polypropylene hole cap | |
packaging | pkg of 72 ea |
vial O.D. × H | 29 mm × 82 mm |
Description:
General description
Each of the vials is cleaned according to US EPA Protocol B: The cleaning procedure is as follows:
1. Wash containers in hot water using laboratory grade non-phosphate detergent.
2. Rinse three times with copious amounts of tap water to remove detergent.
3. Rinse three times with ASTM Type I organic-free water.
4. Oven-dry containers.
5. Allow containers to cool to room temperature in an enclosed contaminant-free environment.
6. Cap the containers.
After being cleaned these vials are assembled with the following materials: Type 1 borosilicate glass, PTFE/silicone septa, and a polypropylene cap. The vials are available in either clear or amber glass. All of the vials are packaged with an open top cap and a tan/white PTFE/silicone septa.
The 40 mL EPA vials are supplied with a Certificate of Analysis and a package of labels to use to identify the contents of your sample. The package of vials is shrink wrapped after cleaning to make sure that the vials arrive to you as clean as possible.
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如常用于缓冲的Tris、HEPES、MPOS等等,去垢剂如NP40,Tween-20,TritonX100,CHAPS,脱氧胆酸钠,SDS等等,
大家能说说这些试剂的使用区别吗(最好讲讲某些情况下使用一些会优于另一些的例子)
有时候某些裂解液含10%左右甘油,又是为何?
平时我们用习惯了一些配方,可能有时候不太清楚为何有些是可替代的,有些是不可替代的,借此机会想听听大家的见解?
去垢剂作为添加剂生长蛋白质晶体是一项很有用的技术。非离子和两性离子结晶剂在膜蛋白结晶中已经得到很好应用,目前已经变成常规的实验手段。去垢剂在一些可溶蛋白中也起到了一定的作用,提高了晶体的质量和结果的可重复性 ,促进了单晶的生长, 但具体的影响机制尚不明确。其可能原因是去垢剂的引入使结晶液滴中的蛋白分子更具有亲水性,使溶液中同质蛋白质分子增加。向左转|向右转
如要膜蛋白保持原来的结构应采用:非离子型去垢剂.


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