-
CAS Number 9003-70-7
Properties:
| Related Categories | Adsorbent Media (Polymeric), Adsorbents (Resins), Amberlite, Amberlite & Amberchrom, Analytical/Chromatography, |
| packaging | pkg of 100 g |
| matrix | styrene-divinylbenzene (macroreticular) |
| particle size | 20-60 mesh |
| pore size | ~0.65 mL/g pore volume |
| 90 Å mean pore size | |
| surface area | ~300 m2/g |
| density | 1.02 g/mL at 25 °C (true wet)(lit.) |
Description:
Frequently Asked Questions
Frequently Asked Questions
are available for this Product.
Application
Polyaromatic adsorbent resin for hydrophobic compounds up to MW 20,000: phenols, organic removal, surfactants, aroma compounds, antibiotic recovery. May be used as support for catalyst or metals removal. For cleaned US EPA versions, see Supelpak-2 and Supelpak-2B.
• Amberlite® XAD®-2 was used as adsorbent in the extraction of organochlorine compounds using Soxhlat extraction technique.
• Amberlite® XAD®-2 was used as adsorbent in the extraction of honey samples using column chromatography for HPLC analysis.
• Amberlite® XAD®-2 was used in adsorption chromatography.
General description
Nonionic macroreticular resin that adsorbs and releases ionic species through hydrophobic and polar interactions; usually used under isocratic conditions.
Specifications:
Amberlite XAD-2, a hydrophobic copolymer of styrene-divinylbenzene resin, requires longer purification process and is commonly used as a sorbent. Amberlite XAD-2 resin sorbs organic compounds depending upon the aromaticity and size of the hydrophobic groups through low-energy physical interactions.
Legal Information
Amberlite is a registered trademark of The Dow Chemical Company or an affiliated company of Dow
XAD is a registered trademark of The Dow Chemical Company or an affiliated company of Dow
Safety:
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
如常用于缓冲的Tris、HEPES、MPOS等等,去垢剂如NP40,Tween-20,TritonX100,CHAPS,脱氧胆酸钠,SDS等等,
大家能说说这些试剂的使用区别吗(最好讲讲某些情况下使用一些会优于另一些的例子)
有时候某些裂解液含10%左右甘油,又是为何?
平时我们用习惯了一些配方,可能有时候不太清楚为何有些是可替代的,有些是不可替代的,借此机会想听听大家的见解?
如要膜蛋白保持原来的结构应采用:非离子型去垢剂.
B.阴离子去垢剂 常见的有十二烷基硫酸钠和十二烷基璜酸钠。前者可促进核蛋白的溶解,将核酸释放出来,并对核酸酶有一定抑制作用,常用于核酸的提取。
C.阳离子去垢剂 如洁尔灭、新洁尔灭、CTAB、CPC、ZEPH、克菌定、消毒净(TMPB)、杜灭芬等,消毒灭菌类居多。
D.天然表面活性剂 又称为生物表面活性剂,包括种类较为广泛,如各种树胶(阿拉伯胶、杏胶、桃胶、果胶)、明胶、皂甙、卵磷脂、豆磷脂、琼脂、海藻酸钠、酪蛋白、胆甾醇、胆酸类、多糖类(如环糊精)等。
E.两性表面活性剂 在碱性水溶液中呈阴离子表面活性剂的性质,起泡性好,去污力也强;在酸性溶液中则呈现阳离子表面活性剂特征,其杀菌性很强。向左转|向右转
我要做的两个目的蛋白,分子量一个是140的,另一个是36,内参用的GAPDH。这几天刚刚要做小鼠组织的蛋白,就把新提取的组织蛋白和之前做过的细胞蛋白一起上了,细胞和组织的各上了3个孔。
电泳转膜孵育等条件都是以前做成熟的
之后显影,细胞蛋白的,两个目的和内参都出得挺好;但是小鼠的组织蛋白只有分子量140的那个显出条带了,36的那个显出来的像一片水渍一样,斑片状的黑,内参有时也这样、有时干脆什么都不显。不知道这是什么原因...按说细胞同样的蛋白做出来,WB的各个步骤应该不会有太大问题的。
有点怀疑蛋白提取的不好,我提组织蛋白的的操作是在冰上进行的,裂解液也加有PMSF,用BCA法测得的蛋白浓度大概也有3~5mg/ml。
在这里求助各位高手~望给我指点一下~不胜感激啊!

![]()
暂无品牌分类
