Description

The most commonly used thiols for the preparation of adsorption-resistant SAMs on gold or other metals (e.g., Ag, Pd, Cu). Ideal as bio-inert background in cell patterning/ spreading studies (Chen et al. Science 276, 1425, 1997), SPR experiments (Mrksich & Whitesides Ann. Rev. Biophys. Biomol. Struct. 25, 55, 1996, Lahiri et al. Langmuir 15, 7186, 1999), protein microarrays (Schaeferling et al. Electrophoresis 23, 3097, 2002) and more.

Resistivity to protein adsorption is similar for different numbers of EG units. Ostuni et al. (Langmuir 17, 5605, 2001) reported the protein adsorptivity of 0.2 % ML (percent monolayer) for pure EG3 SAM. For mixed 1:1 EG/COOH SAMs (cf. Cat. # TH 003) the adsorptivities were: (i) fibrinogen, 1.6 % ML for EG3 and 0.4 % ML for EG6 (ii) Lysozyme, 1.0 % ML for EG3 and 1.0 % ML for EG6. Monolayers prepared from our thiols have even better characteristics with (~0.1 %ML for pure EGn monolayers).

One of the most exciting applications of EG thiols is in the preparation of substrates for cell patterning. This is commonly done by first micropatterning (either by micro contact printing or the ASOMIC technique; cf. scheme below and also Kandere-Grzybowska et al. Nature Methods 2, 739, 2005) an array of cell-adhesive islands of desired shapes and dimensions, and then incubating the rest of the surface with an adhesion-resistant EG thiol. When cells are applied onto such a surface, they stick only to the islands and assume their shapes. In this way, populations of cells of minimal heterogeneity are prepared, and the influence of geometry on cell functioning can be studied in quantitative detail. In their seminal paper (C.S. Chen et al. Science 276, 1425, 1997), Whitesides and Ingber have shown how shape modification can control apoptosis. More recently (Nature Methods 2, 739, 2005), Borisy and Grzybowski groups have demonstrated how it can be used to adjust and control cytoskeletal functions in living cells. The picture below provides a colorful illustration of this capability, with a B16F1 cell made triangular and then fluorescently labeled for microtubules (green), actin stress fibers (blue) and focal adhesions (red).





Notes: (1) The key to successful use of EG monolayers for cell studies is their low cytotoxicity. ProChimia developed special purification methods to ensure that its thiols are extremely "cell friendly". (2) For maximal contrast between the cell islands and the bioresistant portion of the surface surrounding these islands, we recommend the EG6 thiol. (3) Please note that as of May 2006, ProChimia also offers a selection of high-quality gold substrates and PDMS stamps for cell micropatterning. Please contact one of our offices to learn about specific products.

ProChimia

波兰ProChimia成立于2001年。专注于化学领域，纳米技术和生物技术领域，ProChimia为化学和生化各种类型的表面和材料的改性提供了一流的解决方案。产品用于官能化自组装单分子膜的化学物质，以及单分散金属（金、银、铜、铂、钯）和氧化铁纳米粒子功能化与各种配体。产品被全球2000多组织使用，客户包括著名学术机构(哈佛大学、麻省理工学院、斯坦福、伯克利、牛津、ETH等)以及领先的高科技公司（Intel、Fuji、IBM、Qiagene，等等）。

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波兰ProChimia成立于2001年。专注于化学领域，纳米技术和生物技术领域，ProChimia为化学和生化各种类型的表面和材料的改性提供了一流的解决方案。产品用于官能化自组装单分子膜的化学物质，以及单分散金属(金、银、铜、铂、钯)和氧化铁纳米粒子功能化与各种配体。产品被全球2000多组织采用，客户包括著名学术机构(哈佛大学、麻省理工学院、斯坦福、伯克利、牛津、ETH等)以及领先的高科技公司(Intel、Fuji、IBM、Qiagene，等等)。

PROCHIMIA,公司是一家成立於经销商,年並從事2005行業的,进出口—化学和制药公司.它同時從事 

• 化学—进出口
 
• 添加剂、食用色料和色素
 和 
• 包装胶水

 

Prochimia公司将产品的高质量作为最高标准，每一个产品都要通过严格的质量监测体系检测，确保了稳定可靠的产品。目标是提供卓越的产品和高效的技术支持，同时还努力开发更先进的检测系统，保证客户能得到代表最高水平的服务。

从研究与临床需要出发，设计开发产品，真正满足实际要求，产品已被生命科学研究群体所广泛认同；有完善的专业文献资料积累，所有产品都提供详细的技术资料与重要的相关文献；产品提供多种规格,可以适应各种不同要求，此外还可根据实验要求选择特定的产品形式或规格。

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苏州蚂蚁淘-代理销售波兰ProchimiaSurfaces公司的产品




ProchimiaSurfaces

波兰ProChimia成立于2001年。专注于化学领域、纳米技术和生物技术领域，ProChimia为化学和生化各种类型的表面和材料的改性提供了一流的解决方案。产品用于官能化自组装单分子膜的化学物质，以及单分散金属(金、银、铜、铂、钯)和氧化铁纳米粒子功能化与各种配体。产品被全球2000多组织采用，客户包括著名学术机构(哈佛大学、麻省理工学院、斯坦福、伯克利、牛津、ETH等)以及领先的高科技公司(Intel、Fuji、IBM、Qiagene等等)。





Superiorchemicalsforselfassembly,surfacemodificationandnanoparticles.




Acetyl-protectedthiols（乙酰保护硫醇）Calixarenes（杯芳烃）Couplingreagents（偶联试剂）Disulfides（二硫化物）Dithiolsfornanoparticleassembly（纳米颗粒组装用二硫醇）Fluorinatedthiolsandsilanes（氟化硫醇和硅烷）Functionalizedthiols（功能化硫醇）Graphenederivatives（石墨烯衍生物）Ligandsforironoxidesurfaces（氧化铁表面配体）MOFs（Metal-OrganicFrameworks，金属有机框架化合物）Photoswitchablethiols（光开关硫醇）Silanes（硅烷）ThiolatedCarbohydrates(mimickingglycans)（硫代碳水化合物-模拟聚糖）Thiolatedcrownethers（硫化冠醚）ThiolsanddisulfidesforfluorescentSAMs（用于荧光SAMs的硫醇和二硫化物）ThiolsforbioresistantandbiospecificSAMs（用于生物抗药性和生物特异性SAMs的硫醇）ThiolsforelectroactiveSAMs（用于电活性SAMs的硫醇）Thiophoshonates（硫代磷酸酯）Ultra-bioresistantZwitterionicligands（超生物电阻两性离子配体）Usefulsyntheticprecursorsandbuildingblocks（有用的合成前体和组成链段）