VitroCol®
TypeICollagenSolution,3mg/ml(Human)
Catalog#5007
VitroCol®isa3mg/ml,typeIhumanatelocollagensolutionfor2Dcoatings,3Dhydrogels,orasacollagenstandard.VitroCol®collagenisnaturallysecretedfromhumanneo-natalfibroblastcells,thenprocessedandpurifiedtoyieldthenaturallyproducedhumancollagen.VitroCol®collagenisthefirstwidelyavailable,naturallyproducedpurifiedhumancollagenforresearchpurposes.VitroCol®setsthestandardforpurity(99%collagencontent),functionalityandrepresentstheonlynative-likehumancollagenoffered.
VitroCol®collagenisnaturallysecretedfromhumanneo-natalfibroblastcells.Thehumanfibroblastsareculturedinoptimalconditionsallowingthefibroblasttonaturallyandefficientlysecretextracellularmatrix.Theextracellularmatrixisthenprocessedandpurifiedtoyieldthenaturallyproducedhumancollagen.
VitroCol®isapproximately97%TypeIhumancollagenwiththeremainderbeingcomprisedofTypeIIIcollagen.VitroCol®issuppliedatapproximately3mg/mlconcentration.TheconcentrationforeachspecificlotisprovidedonaCertificateofAnalysisthatisavailablewiththepurchaseofeachproduct.VitroCol®issolubleatelocollagenin0.01NHCI,therefore,thepHis2.
VitroCol®isespeciallyidealforhumancellculturesystemswhencoatingofsurfaces,providingpreparationsofthinlayersofculturingcells,oruseasasolidgel.VitroCol®humancollagenisprovidedinuser-friendlypackagingforuseandstorage.VitroCol®issterilefilteredandissuppliedasareadytousesolution.
Parameter,Testing,andMethod
VitroCol®TypeICollagen#5007
SterilizationMethod
Filtration
ExtractionMethod
Enzyme-atelocollagen
Form
Solution
PackageSize
20mL,100mL
StorageTemperature
2-10°C
ShelfLife
Minimumof6monthsfromdateofreceipt
CollagenConcentration-Biuret
2.9-3.2mg/mL
CollagenPurity-SilverStaining
99%
pH
1.9-2.1
KineticGelTest(Minutes)
40
GelFormationTubeTest(Minutes)
40
Fibrillogenesis(AbsorbanceUnits)
0.5
ElectrophoreticPattern-CoomassieBlue
Characteristic
Sterility-USPmodified
Nogrowth
Endotoxin-LAL
5.0EU/mL
Osmolality(mOsmoH2O/kg)
35
CellAttachmentAssay
Pass
Source
HumanNeo-NatalFibroblasts
HydrogelYoungsModulusE(Pa)
Characteristic
ReferencesforVitroCol®:
Shieh,HesterF.,etal.ComparisonsofhumanamnioticmesenchymalstemcellviABIlityinFDA-approvedcollagen-basedscaffolds:Implicationsforengineereddiaphragmaticreplacement.Journalofpediatricsurgery52.6(2017):1010-1013.
vanderVelden,JosLJ,etal.TransformingGrowthFactor-ßInducesAMesenchymalProfileInHumanNasalEpithelialCells.D76.ALVEOLAREPITHELIUM:NOVELTOOLSANDPHENOTYPES.AmericanThoracicSociety,2012.A6322-A6322.
Tashima,Takumi,etal.Osteomodulinregulatesdiameterandaltersshapeofcollagenfibrils.Biochemicalandbiophysicalresearchcommunications463.3(2015):292-296.
Spiller,KaraL.,SuzanneA.Maher,andAnthonyM.Lowman.Hydrogelsfortherepairofarticularcartilagedefects.TissueengineeringpartB:reviews17.4(2011):281-299.
Brilha,Sara,etal.Monocyteadhesion,migration,andextracellularmatrixbreakdownareregulatedbyintegrinαVβ3inMycobacteriumtuberculosisinfection.TheJournalofImmunology199.3(2017):982-991.
Jonsdottir,HuldaR.,andRonaldDijkman.Characterizationofhumancoronavirusesonwell-differentiatedhumanairwayepithelialcellcultures.Coronaviruses.HumanaPress,NewYork,NY,2015.73-87.
Sabbione,Florencia,etal.Neutrophilextracellulartrapsstimulateproinflammatoryresponsesinhumanairwayepithelialcells.Journalofinnateimmunity9.4(2017):387-402.
DosSantosBrilha,S.,etal.Monocyteadhesion,migrationandextracellularmatrixbreakdownisregulatedbyintegrinαVβ3inMycobacteriumtuberculosisinfection.
Brilha,Sara,etal.MonocyteAdhesion,Migration,andExtracellularMatrixBreakdownIsRegulatedbyIntegrinaVb3inMycobacteriumtuberculosisInfection.(2017).
Colace,T.,etal.Analysisofmorphologyofplateletaggregatesformedoncollagenunderlaminarbloodflow.Annalsofbiomedicalengineering39.2(2011):922-929.
Muthard,RyanW.,andScottL.Diamond.Bloodclotsarerapidlyassembledhemodynamicsensors:flowarresttriggersintraluminalthrombuscontraction.Arteriosclerosis,thrombosis,andvascularBIOLOGy32.12(2012):2938-2945.
Maloney,S.F.,LawrenceF.Brass,andS.L.Diamond.P2Y12orP2Y1inhibitorsreduceplateletdepositioninamicrofluidicmodelofthrombosiswhileapyraselacksefficacyunderflowconditions.IntegrativeBiology2.4(2010):183-192.
Bauer,RebeccaN.,etal.Interactionwithepithelialcellsmodifiesairwaymacrophageresponsetoozone.Americanjournalofrespiratorycellandmolecularbiology52.3(2015):285-294.
Kawamura,Shunsuke,etal.IdentificationofcommonmonocyteProgenitors,pre-monocytes,andgranulocytemonocyteprogenitorsinhumanumbilicalcordblood.ExperimentalHematology43.9(2015):S72.
