Catalog#5005

PureCol®isa3mg/ml,TypeIbovineatelocollagensolutionfor2Dcoatings,3Dhydrogels,orasacollagenstandard.PureCol®isknownasthestandardamongcollagenproductsforpurity(≥99.9%collagencontent),consistencyandreproducibility,andiscitedin2000publications.

PureCol®collagenisknownasthestandardofallcollagensforpurity(99.9%collagencontent),functionality,andthemostnative-likecollagenavailable.PureCol®isisolatedfrombovinehidessourcedfromtheonlycontrolled,closedherdintheUnitedStates.AdvancedBiomatrix’smanufacturingprocessescomplywithstringentqualitystandardsthathaveproventoyieldunsurpassedlot-to-lotconsistency.

PureCol®collagenisapproximately97%TypeIatelocollagenwiththeremainderbeingcomprisedofTypeIIIcollagen.PureCol®collagenissuppliedatapproximatelya3mg/mlconcentration.TheconcentrationforeachspecificlotisprovidedonaCertificateofAnalysisthatisavailablewiththepurchaseofeachproduct.PureCol®issolubleatelocollagenin0.01NHCI,therefore,thepHis2.PureCol®collagenisidealforcoatingofsurfaces,providingpreparationofthinlayersforculturingcells,oruseasasolidgel.

PureCol®collagenisprovidedinauser-friendlypackagingforuseandstorage.PureCol®issterilefilteredandissuppliedasareadytousesolution.

Parameter,Testing,andMethod

PureCol®TypeICollagen#5005

SterilizationMethod

Filtration

ExtractionMethod

Enzyme-atelocollagen

Form

Solution

PackageSize

100mL,1000mL

StorageTemperature

2-10°C

ShelfLife

Minimumof6monthsfromdateofreceipt

CollagenConcentration-Biuret

2.9-3.2mg/mL

CollagenPurity-SilverStaining

99.9%

pH

1.9-2.1

KineticGelTest(Minutes)

40

GelFormationTubeTest(Minutes)

40

Fibrillogenesis(AbsorbanceUnits)

0.5

ElectrophoreticPattern-CoomassieBlue

Characteristic

Sterility-USPmodified

Nogrowth

Endotoxin-LAL

1.0EU/mL

Osmolality(mOsmoH2O/kg)

35

CellAttachmentAssay

Pass

Source

BovineHide

HydrogelYoungsModulusE(Pa)

Characteristic

BecausePureCol®hasbeencitedinover2000publications,wehaveonlypostedafewbelow:

Sorensen,JacobR.,etal.Analteredresponseinmacrophagephenotypefollowingdamageinagedhumanskeletalmuscle:implicationsforskeletalmusclerepair.TheFASEBJournal(2019):fj-201900519R.

Sorensen,JacobR.,etal.Analteredresponseinmacrophagephenotypefollowingdamageinagedhumanskeletalmuscle:implicationsforskeletalmusclerepair.TheFASEBJournal(2019):fj-201900519R.

Colaço,E.,etal.HierarchicalCollagen-HydroxyapatiteNanostructuresDesignedThroughLayer-by-LayerAssemblyofCrystal-DecoratedFibrils.J.,HierarchicalCollagen-HydroxyapatiteNanostructuresDesignedThroughLayer-by-LayerAssemblyofCrystal-DecoratedFibrils(May13,2019)(2019).

Schwerdtfeger,LukeA.,etal.Humancolonfunctionexvivo:Dependenceonoxygenandsensitivitytoantibiotic.PloSone14.5(2019):e0217170.

Cardoso,Ana,etal.MiR-144overexpressionasapromisingtherapeuticstrategytoovercomeglioblastomacellinvasivenessandresistancetochemotherapy.Humanmoleculargenetics(2019).

Steele,HannahE.,etal.MechanotransductionofmitochondrialAMPKanditsdistinctroleinflow-inducedbreastcancercellmigration.Biochemicalandbiophysicalresearchcommunications514.2(2019):524-529.

Gehwolf,Renate,etal.GlobalResponsesofIl-1β-Primed3DTendonConstructstoTreatmentwithPulsedElectromagneticFields.Cells8.5(2019):399.

Alexander,Frank,SebastianEggert,andDoriellePrice.Label-FreeMonitoringof3DTissueModelsviaElectricalImpedanceSpectroscopy.(2019):1-24.

Matysik-Woźniak,Anna,etal.ExaminationofKynurenineToxicityonCornealandConjunctivalEpithelium:InvitroandinvivoStudies.Ophthalmicresearch(2019):1-12.

Compton,Clayton,etal.ReconstitutionoftheVentricularEndocardiumWithinAcellularHearts.RegenerativeEngineeringandTranslationalMedicine(2019):1-11.

Müller,A.L.,etal.4.IdentificationofmiR-301ainPrimaryHumanAtrialFibroblastsandBoneMarrow-DerivedMesenchymalProgenitorCellstoAttenuateEndogenousDifferentiationintoPro-FibroticCells.DifferentiationofPrimaryHumanPro-FibroticMesenchymalCellsInfluencedbyExtracellularMatrixEnvironmentDeterminedbyMicro-RNAExpression(2018):130.

Doblinger,Nina,etal.Impactofhydroxyethylstarchandmodifiedfluidgelatinongranulocytephenotypeandfunction.Transfusion(2019).

ELISAbeth,etal.Pro-InflammatoryResponsesinHumanBronchialEpithelialCellsInducedbySporesandHyphalFragmentsofCommonDampIndoorMolds.Internationaljournalofenvironmentalresearchandpublichealth16.6(2019):1085.

Dodmane,PuttappaR.,etal.BiphasicchangesinairwayepithelialcellEGFreceptorbindingandphosphorylationinducedbycomponentsofhogbarndust.Experimentallungresearch44.10(2018):443-454.

McClellan,Alyce,etal.Anovelmechanismfortheprotectionofembryonicstemcellderivedtenocytesfrominflammatorycytokineinterleukin1beta.Scientificreports9(2019).

Wang,Weiling,etal.Aquaporin-3deficiencyslowscystenlargementinexperimentalmousemodelsofautosomaldominantpolycystickidneydisease.TheFASEBJournal(2019):fj-201801338RRR.

Teo,JyeYng,etal.SurfacetetheringofstemcellswithH2O2-responsiveanti-oxidizingcolloidalparticlesforprotectionagainstoxidation-induceddeath.Biomaterials201(2019):1-15.

Gehwolf,Renate,etal.3D-EmbeddedCellCulturestoStudyTendonBIOLOGy.(2019):1-11.