TeloCol®-6TypeICollagenSolution,6mg/ml(Bovine)
Catalog#5225
TeloCol®-6is50mLof6mg/ml,typeIbovinetelocollagensolutionfor2Dcoatingsor3Dhydrogels.Thiskitcomeswithaneutralizationsolutionforeasyhandlingandpreparation.Beingahigherconcentrationtelocollagen,TeloCol®-6willformfirmhydrogels.
ProductDescriptionTeloCol®-6bovinecollagensolutionisderivedfromanacidextractionprocessyieldingatelopeptide-intactcollagen.Thepro-peptideregionsatbothendsofthecollagenchain,N-andC-telopeptideregions,aremaintained.
TeloCol®-6collagenisapproximately95%TypeIcollagenwiththeremainderbeingcomprisedofTypeIIIcollagen.Eachproductincludesabottlecontaining50mlofcollagensolutionaccompaniedwithabottleofpre-formulatedneutralizingsolutionfortheformationofacollagengel.Thisproductissuppliedataconcentrationofapproximately6mg/ml.TeloCol®-6issterilefilteredandprovidedinuser-friendlypackagingforuseandstorage.
TeloCol®-6isidealforcoatingofsurfaces,providingpreparationofthinlayersforculturingcells,oruseasasolidgel.
Parameter,Testing,andMethod
TeloCol®-6TypeICollagen#5225
SterilizationMethod
Filtration
ExtractionMethod
Acid-telocollagen
Form
Solution
PackageSize
50mLKit
StorageTemperatureofCollagen
2-10°C
StorageTemperatureofNeutralizationSolution
RoomTemperature
ShelfLife
Minimumof6monthsfromdateofreceipt
CollagenConcentration-Biuret
5.8-6.8mg/mL
CollagenPurity-SilverStaining
99%
pH
1.9-3.0
KineticGelTest(Minutes)
40
GelFormationTubeTest(Minutes)
40
Fibrillogenesis(AbsorbanceUnits)
0.35
ElectrophoreticPattern-CoomassieBlue
Characteristic
Sterility-USPmodified
Nogrowth
Endotoxin-LAL
10.0EU/mL
Osmolality(mOsmoH2O/kg)
35
CellAttachmentAssay
Pass
Source
BovineHide
HydrogelYoungsModulusE(Pa)
Characteristic
ReferencesforTeloCol®:
Drzewiecki,KathrynE.,etal.“CircularDichroismSpectroscopyofCollagenFibrillogenesis:ANewUseforanOldTechnique.”BiophysicalJournal,vol.111,no.11,2016,pp.2377–2386.,doi:10.1016/j.bpj.2016.10.023
Wingender,Brian,etal.“BiomimeticOrganizationofCollagenMatricestoTemplateBone-likeMicrostructures.”MatrixBIOLOGy,vol.52-54,2016,pp.384–396.,doi:10.1016/j.matbio.2016.02.004.
Burla,F.,Tauber,J.,Dussi,S.,Gucht,J.V.D.Koenderink,G.H.Stressmanagementincompositebiopolymernetworks.NaturePhysics15,549–553(2019).